Chromosome 7 monosomy and deletions in myeloproliferative diseases

Pasquale Tripputi, Barbara Cassani, Rosamaria Alfano, Daniela Graziani, Daniela Cigognini, Patrizia Doi, Monica Bignotto, Gianmarco Corneo, Guido Coggi

Research output: Contribution to journalArticlepeer-review

Abstract

We studied deletion and monosomy of chromosome 7 in 150 patients with myeloproliferative diseases. We found 8/150 patients with monosomy 7 by cytogenetics and 4/150 with deletions of the long arm of chromosome 7 by restriction fragment length polymorphism (RFLP) analysis performed with Southern and polymerase chain reaction. To overcome limitation of RFLP analysis, we restricted loss of heterozygosity study with microsatellites to 45 patients, observing deletion 7q31.1 in 7/45 patients. In all patients with molecular alterations the deletion was observed only in myeloid cells, while the monosomy was detected in both myeloid precursor and lymphocytes. This finding suggests a CD34-totipotent stem cell origin for the monosomy and a colony forming unit - granulocyte, erytrocyte, monocyte, megakaryocytes (CFU-GEMM) stem cell origin for the deletions.

Original languageEnglish
Pages (from-to)735-739
Number of pages5
JournalLeukemia Research
Volume25
Issue number9
DOIs
Publication statusPublished - 2001

Keywords

  • Chromosome 7
  • Cytogenetics
  • Deletion
  • DNA
  • Microsatellite
  • Myelodysplasia
  • Myeloid leukemia

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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