Chromosome aberrations associated with CAD gene amplification in Chinese hamster cultured cells

L. Ottaggio, C. Agnese, S. Bonatti, P. Cavolina, A. De Ambrosis, P. Degan, A. Di Leonardo, M. Miele, R. Randazzo, A. Abbondandolo

Research output: Contribution to journalArticlepeer-review


Eleven sublines with increasing resistance to N-phosphonacetyl-l-aspartate (PALA) were isolated from the V79,B7 Chinese hamster cell line. Aspartate transcarbamylase activity and CAD gene copy number increased with increasing resistance of sublines. In situ hybridization with a DNA probe for the CAD gene showed that the amplified sequences resided in the terminal region of a marker chromosome with elongated q arms. This region stained homogeneously after G-banding. A high incidence of both numerical and structural chromosome aberrations was found in PALA-resistant cells. In hyperdiploid and polyploid cells, containing 2 copies of the marker chromosome, dicentrics were found at a very high frequency. As indicated by in situ hybridization and G-banding, they originated from a rearrangement involving 2 homologous marker chromosomes.

Original languageEnglish
Pages (from-to)111-121
Number of pages11
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Issue number1
Publication statusPublished - 1988


  • CAD
  • Chromosome aberrations
  • Gene amplification
  • PALA resistance

ASJC Scopus subject areas

  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)
  • Genetics
  • Toxicology


Dive into the research topics of 'Chromosome aberrations associated with CAD gene amplification in Chinese hamster cultured cells'. Together they form a unique fingerprint.

Cite this