Abstract
Eleven sublines with increasing resistance to N-phosphonacetyl-l-aspartate (PALA) were isolated from the V79,B7 Chinese hamster cell line. Aspartate transcarbamylase activity and CAD gene copy number increased with increasing resistance of sublines. In situ hybridization with a DNA probe for the CAD gene showed that the amplified sequences resided in the terminal region of a marker chromosome with elongated q arms. This region stained homogeneously after G-banding. A high incidence of both numerical and structural chromosome aberrations was found in PALA-resistant cells. In hyperdiploid and polyploid cells, containing 2 copies of the marker chromosome, dicentrics were found at a very high frequency. As indicated by in situ hybridization and G-banding, they originated from a rearrangement involving 2 homologous marker chromosomes.
Original language | English |
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Pages (from-to) | 111-121 |
Number of pages | 11 |
Journal | Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis |
Volume | 199 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1988 |
Keywords
- CAD
- Chromosome aberrations
- Gene amplification
- PALA resistance
ASJC Scopus subject areas
- Molecular Biology
- Health, Toxicology and Mutagenesis
- Medicine(all)
- Genetics
- Toxicology