Chromosome aberrations associated with CAD gene amplification in Chinese hamster cultured cells

L. Ottaggio; C. Agnese; S. Bonatti; P. Cavolina; A. De Ambrosis; P. Degan; A. Di Leonardo; M. Miele; R. Randazzo; A. Abbondandolo

doi:10.1016/0027-5107(88)90236-9

Chromosome aberrations associated with CAD gene amplification in Chinese hamster cultured cells

L. Ottaggio, C. Agnese, S. Bonatti, P. Cavolina, A. De Ambrosis, P. Degan, A. Di Leonardo, M. Miele, R. Randazzo, A. Abbondandolo

A.O.U. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA)

Research output: Contribution to journal › Article › peer-review

Abstract

Eleven sublines with increasing resistance to N-phosphonacetyl-l-aspartate (PALA) were isolated from the V79,B7 Chinese hamster cell line. Aspartate transcarbamylase activity and CAD gene copy number increased with increasing resistance of sublines. In situ hybridization with a DNA probe for the CAD gene showed that the amplified sequences resided in the terminal region of a marker chromosome with elongated q arms. This region stained homogeneously after G-banding. A high incidence of both numerical and structural chromosome aberrations was found in PALA-resistant cells. In hyperdiploid and polyploid cells, containing 2 copies of the marker chromosome, dicentrics were found at a very high frequency. As indicated by in situ hybridization and G-banding, they originated from a rearrangement involving 2 homologous marker chromosomes.

Original language	English
Pages (from-to)	111-121
Number of pages	11
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	199
Issue number	1
DOIs	https://doi.org/10.1016/0027-5107(88)90236-9
Publication status	Published - 1988

Keywords

CAD
Chromosome aberrations
Gene amplification
PALA resistance

ASJC Scopus subject areas

Molecular Biology
Health, Toxicology and Mutagenesis
Medicine(all)
Genetics
Toxicology

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10.1016/0027-5107(88)90236-9

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Ottaggio, L., Agnese, C., Bonatti, S., Cavolina, P., De Ambrosis, A., Degan, P., Di Leonardo, A., Miele, M., Randazzo, R., & Abbondandolo, A. (1988). Chromosome aberrations associated with CAD gene amplification in Chinese hamster cultured cells. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 199(1), 111-121. https://doi.org/10.1016/0027-5107(88)90236-9

Ottaggio, L, Agnese, C, Bonatti, S, Cavolina, P, De Ambrosis, A, Degan, P, Di Leonardo, A, Miele, M, Randazzo, R & Abbondandolo, A 1988, 'Chromosome aberrations associated with CAD gene amplification in Chinese hamster cultured cells', Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, vol. 199, no. 1, pp. 111-121. https://doi.org/10.1016/0027-5107(88)90236-9

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title = "Chromosome aberrations associated with CAD gene amplification in Chinese hamster cultured cells",

abstract = "Eleven sublines with increasing resistance to N-phosphonacetyl-l-aspartate (PALA) were isolated from the V79,B7 Chinese hamster cell line. Aspartate transcarbamylase activity and CAD gene copy number increased with increasing resistance of sublines. In situ hybridization with a DNA probe for the CAD gene showed that the amplified sequences resided in the terminal region of a marker chromosome with elongated q arms. This region stained homogeneously after G-banding. A high incidence of both numerical and structural chromosome aberrations was found in PALA-resistant cells. In hyperdiploid and polyploid cells, containing 2 copies of the marker chromosome, dicentrics were found at a very high frequency. As indicated by in situ hybridization and G-banding, they originated from a rearrangement involving 2 homologous marker chromosomes.",

keywords = "CAD, Chromosome aberrations, Gene amplification, PALA resistance",

author = "L. Ottaggio and C. Agnese and S. Bonatti and P. Cavolina and {De Ambrosis}, A. and P. Degan and {Di Leonardo}, A. and M. Miele and R. Randazzo and A. Abbondandolo",

year = "1988",

doi = "10.1016/0027-5107(88)90236-9",

language = "English",

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T1 - Chromosome aberrations associated with CAD gene amplification in Chinese hamster cultured cells

AU - Ottaggio, L.

AU - Agnese, C.

AU - Bonatti, S.

AU - Cavolina, P.

AU - De Ambrosis, A.

AU - Degan, P.

AU - Di Leonardo, A.

AU - Miele, M.

AU - Randazzo, R.

AU - Abbondandolo, A.

PY - 1988

Y1 - 1988

N2 - Eleven sublines with increasing resistance to N-phosphonacetyl-l-aspartate (PALA) were isolated from the V79,B7 Chinese hamster cell line. Aspartate transcarbamylase activity and CAD gene copy number increased with increasing resistance of sublines. In situ hybridization with a DNA probe for the CAD gene showed that the amplified sequences resided in the terminal region of a marker chromosome with elongated q arms. This region stained homogeneously after G-banding. A high incidence of both numerical and structural chromosome aberrations was found in PALA-resistant cells. In hyperdiploid and polyploid cells, containing 2 copies of the marker chromosome, dicentrics were found at a very high frequency. As indicated by in situ hybridization and G-banding, they originated from a rearrangement involving 2 homologous marker chromosomes.

AB - Eleven sublines with increasing resistance to N-phosphonacetyl-l-aspartate (PALA) were isolated from the V79,B7 Chinese hamster cell line. Aspartate transcarbamylase activity and CAD gene copy number increased with increasing resistance of sublines. In situ hybridization with a DNA probe for the CAD gene showed that the amplified sequences resided in the terminal region of a marker chromosome with elongated q arms. This region stained homogeneously after G-banding. A high incidence of both numerical and structural chromosome aberrations was found in PALA-resistant cells. In hyperdiploid and polyploid cells, containing 2 copies of the marker chromosome, dicentrics were found at a very high frequency. As indicated by in situ hybridization and G-banding, they originated from a rearrangement involving 2 homologous marker chromosomes.

KW - CAD

KW - Chromosome aberrations

KW - Gene amplification

KW - PALA resistance

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JO - Mutation Research

JF - Mutation Research

SN - 0027-5107

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Chromosome aberrations associated with CAD gene amplification in Chinese hamster cultured cells

Abstract

Keywords

ASJC Scopus subject areas

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