Chromosome mapping in Cryptosporidium parvum and establishment of a long-range restriction map for chromosome VI

Lorenza Putignani, Pina Sallicandro, Pietro Alano, Mitchell S. Abrahamsen, Andrea Crisanti, Furio Spano

Research output: Contribution to journalArticlepeer-review

Abstract

We used contour-clamped homogeneous electric field (CHEF) gel electrophoresis and Southern blot hybridization to analyze the molecular karyotype of Cryptosporidium parvum and establish the chromosomal location of 12 single copy genes. In agreement with previous studies, the molecular karyotype of C. parvum was found to consist of partially co-migrating chromosomes ranging in size from 0.97 to 1.55 Mb and segregating into five distinct electrophoretic bands. Hybridization results allowed the definition of a linkage group comprised of five distinct loci located on chromosome VI. Southern hybridization and restriction analysis of total C. parvum chromosomes or isolated chromosome VI using gene-specific probes and an oligonucleotide specific for C. parvum telomeres allowed the development of a long-range restriction map of chromosome VI. Copyright (C) 1999 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)231-238
Number of pages8
JournalFEMS Microbiology Letters
Volume175
Issue number2
DOIs
Publication statusPublished - Jun 15 1999

Keywords

  • Chromosomal location
  • Contour-clamped homogeneous electric field gel electrophoresis
  • Cryptosporidium parvum
  • Long-range restriction map
  • Molecular karyotype
  • Telomeric repeat

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Fingerprint

Dive into the research topics of 'Chromosome mapping in Cryptosporidium parvum and establishment of a long-range restriction map for chromosome VI'. Together they form a unique fingerprint.

Cite this