Chromosome-specific accumulation of aneuploidy in the aging mouse brain

Francesca Faggioli, Tao Wang, Jan Vijg, Cristina Montagna

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Chromosomal aneuploidy, the gain or loss of whole chromosomes, is a hallmark of pathological conditions and a causal factor of birth defects and cancer. A number of studies indicate that aneuploid cells are present at a high frequency in the brain of mice and humans, suggesting that mosaic aneuploidies are compatible with normal brain function and prompting the question about their consequences. To explore the possible contribution of aneuploidy to functional decline and loss of cognitive functions during aging, we used a quantitative, dual-labeling interphase-fluorescence in situ hybridization approach to compare aneuploidy levels of chromosomes 1, 7, 14, 15, 16, 18, 19 and Y in the cerebral cortex of 4- and 28-month-old mice. We show that aneuploidy accumulates with age in a chromosome-specific manner, with chromosomes 7, 18 and Y most severely affected, i.e. up to 9.8% of non-neuronal brain nuclei in 28-month-old animals for chromosome 18. While at early age, both neuronal and glial cells are affected equally, the age-related increase was limited to the non-neuronal nuclei. No age-related increase in aneuploidy was observed in the cerebellum or in the spleen of the same animals. Extrapolating the average frequencies of aneuploidy from the average over 8 chromosomes to all 20 mouse chromosomes would indicate an almost 50% aneuploidy frequency in aged mouse brain. Such high levels of genome instability could well be a factor in age-related neurodegeneration.

Original languageEnglish
Article numberdds375
Pages (from-to)5246-5253
Number of pages8
JournalHuman Molecular Genetics
Volume21
Issue number24
DOIs
Publication statusPublished - Dec 2012

Fingerprint

Aneuploidy
Chromosomes
Brain
Chromosomes, Human, Pair 18
Chromosomes, Human, Pair 7
Chromosomes, Human, Pair 20
Chromosomes, Human, Pair 8
Genomic Instability
Chromosomes, Human, Pair 1
Age Factors
Interphase
Fluorescence In Situ Hybridization
Neuroglia
Cerebral Cortex
Cerebellum
Cognition
Spleen

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology

Cite this

Chromosome-specific accumulation of aneuploidy in the aging mouse brain. / Faggioli, Francesca; Wang, Tao; Vijg, Jan; Montagna, Cristina.

In: Human Molecular Genetics, Vol. 21, No. 24, dds375, 12.2012, p. 5246-5253.

Research output: Contribution to journalArticle

Faggioli, Francesca ; Wang, Tao ; Vijg, Jan ; Montagna, Cristina. / Chromosome-specific accumulation of aneuploidy in the aging mouse brain. In: Human Molecular Genetics. 2012 ; Vol. 21, No. 24. pp. 5246-5253.
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