Chromosome walkine on the TCL1 locus involved in T-cell neoplasia

Laura Virgilio, Masaharu Isobe, Maria Grazia Narducci, Patrizia Carotenuto, Barbara Camerini, Nobuyuki Kurosawa, [No Value] Abbas-ar-Rushdi, Carlo M. Croce, Giandomenico Russo

Research output: Contribution to journalArticle

Abstract

The TCL1 locus on chromosome 14 band q32.1 is frequently involved in the chromosomal translocations and inversions with the T-cell receptor genes observed in several T-cell tumors, including T-prolymphocytic leukemias, acute and chronic leukemias associated with the immunodeficiency syndrome ataxia-telangiectasia, and adult T-cell leukemia. All breakpoints cloned in this area have been mapped to 14q32.1, an area distant ≈10,000 kb from the immunoglobulin heavy-chain gene locus on chromosome 14q band 32.3. Except for two cases of inversion, no physical linkage of the cloned breakpoints has been reported, nor has a gene been identified in this region. Taking advantage of chromosome-walking techniques and of the P1 phage, we cloned and characterized 450 kb of the germ-line TCL1 locus, starting from the breakpoints of two independent T-cell leukemias. We show that all molecular rearrangements characterized so far map to these clones, indicating not only that this region is the target of chromosomal rearrangements occurring in this area but also that both inversion and translocations occur within a 300-kb region in the T-cell leukemias. In the attempt to identify a candidate oncogene responsible for the malignant transformation, a CpG island centromeric to the inversions and to the translocations has been identified. Two probes near the CpG island have detected sequences conserved among species, as well as two transcripts in the K562 human erythroleukemia cell line. On the basis of these data, a model of activation of the putative TCL1 oncogene is suggested.

Original languageEnglish
Pages (from-to)9275-9279
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number20
Publication statusPublished - Oct 15 1993

Fingerprint

T-Cell Leukemia
CpG Islands
Oncogenes
Chromosome Walking
Prolymphocytic Leukemia
Chromosomes
Bacteriophage P1
Immunoglobulin Heavy Chain Genes
T-Lymphocytes
T-Cell Receptor Genes
Chromosomes, Human, Pair 14
Adult T Cell Leukemia Lymphoma
Ataxia Telangiectasia
Leukemia, Erythroblastic, Acute
Genetic Translocation
Conserved Sequence
Germ Cells
Neoplasms
Leukemia
Clone Cells

Keywords

  • Chromosome translocations
  • Human chromosome 14 band q32.1
  • Oncogene activation

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Chromosome walkine on the TCL1 locus involved in T-cell neoplasia. / Virgilio, Laura; Isobe, Masaharu; Narducci, Maria Grazia; Carotenuto, Patrizia; Camerini, Barbara; Kurosawa, Nobuyuki; Abbas-ar-Rushdi, [No Value]; Croce, Carlo M.; Russo, Giandomenico.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 20, 15.10.1993, p. 9275-9279.

Research output: Contribution to journalArticle

Virgilio, L, Isobe, M, Narducci, MG, Carotenuto, P, Camerini, B, Kurosawa, N, Abbas-ar-Rushdi, NV, Croce, CM & Russo, G 1993, 'Chromosome walkine on the TCL1 locus involved in T-cell neoplasia', Proceedings of the National Academy of Sciences of the United States of America, vol. 90, no. 20, pp. 9275-9279.
Virgilio, Laura ; Isobe, Masaharu ; Narducci, Maria Grazia ; Carotenuto, Patrizia ; Camerini, Barbara ; Kurosawa, Nobuyuki ; Abbas-ar-Rushdi, [No Value] ; Croce, Carlo M. ; Russo, Giandomenico. / Chromosome walkine on the TCL1 locus involved in T-cell neoplasia. In: Proceedings of the National Academy of Sciences of the United States of America. 1993 ; Vol. 90, No. 20. pp. 9275-9279.
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AU - Camerini, Barbara

AU - Kurosawa, Nobuyuki

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AU - Croce, Carlo M.

AU - Russo, Giandomenico

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N2 - The TCL1 locus on chromosome 14 band q32.1 is frequently involved in the chromosomal translocations and inversions with the T-cell receptor genes observed in several T-cell tumors, including T-prolymphocytic leukemias, acute and chronic leukemias associated with the immunodeficiency syndrome ataxia-telangiectasia, and adult T-cell leukemia. All breakpoints cloned in this area have been mapped to 14q32.1, an area distant ≈10,000 kb from the immunoglobulin heavy-chain gene locus on chromosome 14q band 32.3. Except for two cases of inversion, no physical linkage of the cloned breakpoints has been reported, nor has a gene been identified in this region. Taking advantage of chromosome-walking techniques and of the P1 phage, we cloned and characterized 450 kb of the germ-line TCL1 locus, starting from the breakpoints of two independent T-cell leukemias. We show that all molecular rearrangements characterized so far map to these clones, indicating not only that this region is the target of chromosomal rearrangements occurring in this area but also that both inversion and translocations occur within a 300-kb region in the T-cell leukemias. In the attempt to identify a candidate oncogene responsible for the malignant transformation, a CpG island centromeric to the inversions and to the translocations has been identified. Two probes near the CpG island have detected sequences conserved among species, as well as two transcripts in the K562 human erythroleukemia cell line. On the basis of these data, a model of activation of the putative TCL1 oncogene is suggested.

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