Chromosomes bearing amplified genes are a preferential target of chemicals inducing chromosome breakage and aneuploidy

Laura Ottagio, Stefania Bonati, Zita Cavalieri, Angelo Abbondandolo

Research output: Contribution to journalArticle

Abstract

Micronuclei were induced in V79 Chinese hamster cells and in PALA L and MTX M, two derivative cell lines harboring amplified genes, with 1,3-bis(2-chloroethyl)nitrosourea (BCNU) and vinblastine. Spontaneous and induced micronuclei were analyzed for the presence of centromeres by immunofluorescent CREST staining. Micronuclei formed in PALA L cells were also analyzed for the presence of amplified DNA by in situ hybridization with a CAD gene probe. Both cell lines containing amplified genes showed increased micronucleus induction by BCNU and vinblastine. The marker chromosome of PALA L cells was found to be a preferential target for both the clastogenic and the aneugenic action of the two chemicals, DNA amplification seems therefore to be a destabilizing factor of chromosomal structural integrity and mitotic segregation.

Original languageEnglish
Pages (from-to)149-155
Number of pages7
JournalMutation Research Letters
Volume301
Issue number3
DOIs
Publication statusPublished - 1993

Keywords

  • Chromosome instability
  • Gene amplification
  • Micronuclei

ASJC Scopus subject areas

  • Genetics
  • Toxicology

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