Chromosomes bearing amplified genes are a preferential target of chemicals inducing chromosome breakage and aneuploidy

Laura Ottagio, Stefania Bonati, Zita Cavalieri, Angelo Abbondandolo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Micronuclei were induced in V79 Chinese hamster cells and in PALA L and MTX M, two derivative cell lines harboring amplified genes, with 1,3-bis(2-chloroethyl)nitrosourea (BCNU) and vinblastine. Spontaneous and induced micronuclei were analyzed for the presence of centromeres by immunofluorescent CREST staining. Micronuclei formed in PALA L cells were also analyzed for the presence of amplified DNA by in situ hybridization with a CAD gene probe. Both cell lines containing amplified genes showed increased micronucleus induction by BCNU and vinblastine. The marker chromosome of PALA L cells was found to be a preferential target for both the clastogenic and the aneugenic action of the two chemicals, DNA amplification seems therefore to be a destabilizing factor of chromosomal structural integrity and mitotic segregation.

Original languageEnglish
Pages (from-to)149-155
Number of pages7
JournalMutation Research Letters
Volume301
Issue number3
DOIs
Publication statusPublished - 1993

Fingerprint

NSC 224131
Bearings (structural)
Chromosome Breakage
Aneuploidy
Chromosomes
Vinblastine
Genes
Cells
Pharmacologic Actions
Cell Line
Centromere
DNA
Structural integrity
Cricetulus
Genetic Markers
In Situ Hybridization
Amplification
Computer aided design
Staining and Labeling
Derivatives

Keywords

  • Chromosome instability
  • Gene amplification
  • Micronuclei

ASJC Scopus subject areas

  • Genetics
  • Toxicology

Cite this

Chromosomes bearing amplified genes are a preferential target of chemicals inducing chromosome breakage and aneuploidy. / Ottagio, Laura; Bonati, Stefania; Cavalieri, Zita; Abbondandolo, Angelo.

In: Mutation Research Letters, Vol. 301, No. 3, 1993, p. 149-155.

Research output: Contribution to journalArticle

Ottagio, Laura ; Bonati, Stefania ; Cavalieri, Zita ; Abbondandolo, Angelo. / Chromosomes bearing amplified genes are a preferential target of chemicals inducing chromosome breakage and aneuploidy. In: Mutation Research Letters. 1993 ; Vol. 301, No. 3. pp. 149-155.
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