Chronic alcohol abuse and endosseous implants: Linkage of in vitro osteoblast dysfunction to titanium osseointegration rate

Paola Torricelli, Milena Fini, Gianluca Giavaresi, Lia Rimondini, Matilde Tschon, Roberto Rimondini, Antonio Carrassi, Roberto Giardino

Research output: Contribution to journalArticle

Abstract

Chronic alcohol consumption is associated with pathological effects on bone, and it is correlated with the increasing risk of osteoporosis and fractures. The negative effects of alcohol intake also influence bone repair processes and the osseointegration of implants. The aim of the present in vitro study was to investigate the proliferation and synthetic activity of osteoblasts isolated from the trabecular bone of rats previously exposed to 7-week intermittent exposure to ethanol vapour (EE-OB), and sham-aged rats (SA-OB), when cultured on standard commercially pure Ti (cpTi). Osteoblast proliferation (WST-1), alkaline phosphatase (ALP), osteocalcin (OC), collagen type I (CICP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and transforming growth factor-β1 (TGF-β1) were measured at 1, 7, and 14 days of culture. Our results showed a decrease in the cell viability and synthetic activity of osteoblasts exposed to ethanol when cultured on cpTi. Moreover, the release of local regulatory factors from osteoblasts was imbalanced: TGF-β1 production was reduced and TNF-α and IL-6 were up-regulated. These in vitro data suggest that alcohol abuse affects bone repair and decreases the ability to form bone around standard cpTi. Innovative surfaces and adjuvant therapies could be useful when implants are required in alcoholics.

Original languageEnglish
Pages (from-to)138-144
Number of pages7
JournalToxicology
Volume243
Issue number1-2
DOIs
Publication statusPublished - Jan 14 2008

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Osseointegration
Osteoblasts
Titanium
Alcoholism
Bone
Alcohols
Bone and Bones
Transforming Growth Factors
Interleukin-6
Ethanol
Tumor Necrosis Factor-alpha
Rats
Repair
Aptitude
Osteocalcin
Alcoholics
Collagen Type I
Alcohol Drinking
Osteoporosis
Alkaline Phosphatase

Keywords

  • Alcohol
  • Biomaterials
  • Osseointegration
  • Osteoblasts
  • Titanium

ASJC Scopus subject areas

  • Toxicology

Cite this

Chronic alcohol abuse and endosseous implants : Linkage of in vitro osteoblast dysfunction to titanium osseointegration rate. / Torricelli, Paola; Fini, Milena; Giavaresi, Gianluca; Rimondini, Lia; Tschon, Matilde; Rimondini, Roberto; Carrassi, Antonio; Giardino, Roberto.

In: Toxicology, Vol. 243, No. 1-2, 14.01.2008, p. 138-144.

Research output: Contribution to journalArticle

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