Abstract
After long-term denervation in mixed rat muscles there is a selective loss of slow myosin. The bidimensional electrophoretic pattern of light chains and the results of preliminary studies on heavy chains have left open the question of whether the nature of the residual fast-like myosin is of the immature or adult type. We have further investigated chronically denervated myosin by (1) electrophoresis in nondissociating conditions; (2) acidic electrophoresis of the heavy chains; and (3) proteolytic mapping of the heavy chains. These techniques clearly distinguish adult myosins from those present in immature muscles. Using these criteria, myosin from the chronically denervated diaphragm is of an adult type, even though the presence of trace amounts of embryonic myosin cannot be excluded. The contractile properties also indicate that chronically denervated hemidiaphragm is more similar to an adult fast muscle than to an immature muscle. The selective maintenance after long-term denervation of fast myosin in adult muscle provides good evidence of the independence of the genetic expression of myosin from a direct neural chemotrophic control.
| Original language | English |
|---|---|
| Pages (from-to) | 515-524 |
| Number of pages | 10 |
| Journal | Muscle and Nerve |
| Volume | 5 |
| Issue number | 7 |
| Publication status | Published - 1982 |
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ASJC Scopus subject areas
- Clinical Neurology
- Neuroscience(all)
Cite this
Chronic denervation of rat diaphragm : Selective maintenance of adult fast myosin heavy chains. / Carraro, U.; Libera, L. D.; Catani, C.; Daniele-Betti, D.
In: Muscle and Nerve, Vol. 5, No. 7, 1982, p. 515-524.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chronic denervation of rat diaphragm
T2 - Selective maintenance of adult fast myosin heavy chains
AU - Carraro, U.
AU - Libera, L. D.
AU - Catani, C.
AU - Daniele-Betti, D.
PY - 1982
Y1 - 1982
N2 - After long-term denervation in mixed rat muscles there is a selective loss of slow myosin. The bidimensional electrophoretic pattern of light chains and the results of preliminary studies on heavy chains have left open the question of whether the nature of the residual fast-like myosin is of the immature or adult type. We have further investigated chronically denervated myosin by (1) electrophoresis in nondissociating conditions; (2) acidic electrophoresis of the heavy chains; and (3) proteolytic mapping of the heavy chains. These techniques clearly distinguish adult myosins from those present in immature muscles. Using these criteria, myosin from the chronically denervated diaphragm is of an adult type, even though the presence of trace amounts of embryonic myosin cannot be excluded. The contractile properties also indicate that chronically denervated hemidiaphragm is more similar to an adult fast muscle than to an immature muscle. The selective maintenance after long-term denervation of fast myosin in adult muscle provides good evidence of the independence of the genetic expression of myosin from a direct neural chemotrophic control.
AB - After long-term denervation in mixed rat muscles there is a selective loss of slow myosin. The bidimensional electrophoretic pattern of light chains and the results of preliminary studies on heavy chains have left open the question of whether the nature of the residual fast-like myosin is of the immature or adult type. We have further investigated chronically denervated myosin by (1) electrophoresis in nondissociating conditions; (2) acidic electrophoresis of the heavy chains; and (3) proteolytic mapping of the heavy chains. These techniques clearly distinguish adult myosins from those present in immature muscles. Using these criteria, myosin from the chronically denervated diaphragm is of an adult type, even though the presence of trace amounts of embryonic myosin cannot be excluded. The contractile properties also indicate that chronically denervated hemidiaphragm is more similar to an adult fast muscle than to an immature muscle. The selective maintenance after long-term denervation of fast myosin in adult muscle provides good evidence of the independence of the genetic expression of myosin from a direct neural chemotrophic control.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019989127&partnerID=8YFLogxK
M3 - Article
C2 - 7144808
AN - SCOPUS:0019989127
VL - 5
SP - 515
EP - 524
JO - Muscle and Nerve
JF - Muscle and Nerve
SN - 0148-639X
IS - 7
ER -