Chronic ethanol administration induces oxidative alterations and functional impairment of pancreatic mitochondria in the rat

Ignazio Grattagliano, Vincenzo Palmieri, Gianluigi Vendemiale, Piero Portincasa, Emanuele Altomare, Giuseppe Palasciano

Research output: Contribution to journalArticle

Abstract

Pancreas is known to be vulnerable to ethanol (ETOH) at high doses, but little is known about the effect of lower doses. Therefore, in this study, the levels of glutathione (GSH), sulfhydryl proteins (P-SH), carbonyl proteins, malondialdehyde (MDA), and adenosine triphosphate (ATP) production were determined in the pancreatic tissue and mitochondria of rats fed for 8 weeks with 3% ETOH. ETOH decreased the pancreatic pool of ATP, while GSH and P-SH also decreased in mitochondria. MDA concentrations increased both in the pancreas and mitochondria, while carbonyl proteins increased only in mitochondria. Pancreatic cells of ETOH-treated rats were frequently affected by cytoplasmic vacuolization and swelling; these alterations were often associated with ductular dilatation. In conclusion, ETOH produces oxidative and morphologic alterations in the pancreas of the rat, including impairment of mitochondria. These effects may represent basic mechanisms of ETOH-induced pancreatic injury.

Original languageEnglish
Pages (from-to)549-553
Number of pages5
JournalDigestion
Volume60
Issue number6
DOIs
Publication statusPublished - Nov 1999

Keywords

  • Adenosine triphosphate
  • Alcoholic pancreatic injury
  • Glutathione
  • Lipid peroxidation
  • Mitochondria
  • Protein oxidation

ASJC Scopus subject areas

  • Gastroenterology

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    Grattagliano, I., Palmieri, V., Vendemiale, G., Portincasa, P., Altomare, E., & Palasciano, G. (1999). Chronic ethanol administration induces oxidative alterations and functional impairment of pancreatic mitochondria in the rat. Digestion, 60(6), 549-553. https://doi.org/10.1159/000007705