Abstract
Chronic granulomatous disease includes a group of genetically inhereted diseases in which there is a reduced capacity of phagocytes to release union superoxide (O2 -) upon infection of cells with microrganisms or chemotactic factors. Diagnosis depend upon demonstration of reduced production of O2 - by neutrophils activated with phorbol-myrestate-acetate (PMA). Nitro blue tetrazolium (NBT) reduction test is the commonly used tecniquefor the identification of CGD patients. However, this method does not allow the identification of patients with variant-CGD that display a residual production of reactive oxygen. Recently, novel tests for the diagnosis of CGD (based on flow-cytometry analysis) have been introduced. We investigated anion superoxide production in PMA-activated neutrophils of 28 healthy adult donors, 30 children with the clinical suspect of CGD, and 5 of the X-linked form of CGD (X-CGD) by flow cytometric with dihydrorhodamine 123, NBT salts precipitation and dismutase-inhibitable cytochrome-c reduction. Each of these assays allowed correct identification of 5 CGD patients among 30 studied, although the NBT test failed to recognize a low production of O2 - in a patient then found affected from a variant form of the disease. These three diagnostic assays were then used to study anion superoxide release in neutrophils derived from 7 mothers or sisters of X-CGD patients. Interestingly, in one of the CGD carriers we found that about 6% of DHR-loaded granulocytes failed to respond to PMA stimulation, thus indicating that this subject was an X-CGD carrier as confirmed by mutational analysis of CYBB gene. In contrast, the both cytochrome- c reduction test and the NBT test failed to detect the carrier state in this subject. On the basis of our results we conclude that flow cytometric analysis of anion superoxide production by PMN, is an effective, rapid and accurate tool for diagnosis of Chronic Granulomatous Disease and allows precise identification of X-CGD-carrier.
| Original language | English |
|---|---|
| Pages (from-to) | 1029-1035 |
| Number of pages | 7 |
| Journal | Rivista Italiana di Pediatria |
| Volume | 23 |
| Issue number | 6 |
| Publication status | Published - 1997 |
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Keywords
- Anion superoxide
- Chronic Granulomatous Disease
- Diagnosis, flow citometry
- Neutrophils
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health
Cite this
Chronic Granulomatous Disease (CGD) identification and carrier state detection by flow cytometric analysis of granulocyte respiratory burst. / Bresciani, S.; Badolato, R.; Verardi, R.; Ugazio, A. G.; Notarangelo, L. D.
In: Rivista Italiana di Pediatria, Vol. 23, No. 6, 1997, p. 1029-1035.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chronic Granulomatous Disease (CGD) identification and carrier state detection by flow cytometric analysis of granulocyte respiratory burst
AU - Bresciani, S.
AU - Badolato, R.
AU - Verardi, R.
AU - Ugazio, A. G.
AU - Notarangelo, L. D.
PY - 1997
Y1 - 1997
N2 - Chronic granulomatous disease includes a group of genetically inhereted diseases in which there is a reduced capacity of phagocytes to release union superoxide (O2 -) upon infection of cells with microrganisms or chemotactic factors. Diagnosis depend upon demonstration of reduced production of O2 - by neutrophils activated with phorbol-myrestate-acetate (PMA). Nitro blue tetrazolium (NBT) reduction test is the commonly used tecniquefor the identification of CGD patients. However, this method does not allow the identification of patients with variant-CGD that display a residual production of reactive oxygen. Recently, novel tests for the diagnosis of CGD (based on flow-cytometry analysis) have been introduced. We investigated anion superoxide production in PMA-activated neutrophils of 28 healthy adult donors, 30 children with the clinical suspect of CGD, and 5 of the X-linked form of CGD (X-CGD) by flow cytometric with dihydrorhodamine 123, NBT salts precipitation and dismutase-inhibitable cytochrome-c reduction. Each of these assays allowed correct identification of 5 CGD patients among 30 studied, although the NBT test failed to recognize a low production of O2 - in a patient then found affected from a variant form of the disease. These three diagnostic assays were then used to study anion superoxide release in neutrophils derived from 7 mothers or sisters of X-CGD patients. Interestingly, in one of the CGD carriers we found that about 6% of DHR-loaded granulocytes failed to respond to PMA stimulation, thus indicating that this subject was an X-CGD carrier as confirmed by mutational analysis of CYBB gene. In contrast, the both cytochrome- c reduction test and the NBT test failed to detect the carrier state in this subject. On the basis of our results we conclude that flow cytometric analysis of anion superoxide production by PMN, is an effective, rapid and accurate tool for diagnosis of Chronic Granulomatous Disease and allows precise identification of X-CGD-carrier.
AB - Chronic granulomatous disease includes a group of genetically inhereted diseases in which there is a reduced capacity of phagocytes to release union superoxide (O2 -) upon infection of cells with microrganisms or chemotactic factors. Diagnosis depend upon demonstration of reduced production of O2 - by neutrophils activated with phorbol-myrestate-acetate (PMA). Nitro blue tetrazolium (NBT) reduction test is the commonly used tecniquefor the identification of CGD patients. However, this method does not allow the identification of patients with variant-CGD that display a residual production of reactive oxygen. Recently, novel tests for the diagnosis of CGD (based on flow-cytometry analysis) have been introduced. We investigated anion superoxide production in PMA-activated neutrophils of 28 healthy adult donors, 30 children with the clinical suspect of CGD, and 5 of the X-linked form of CGD (X-CGD) by flow cytometric with dihydrorhodamine 123, NBT salts precipitation and dismutase-inhibitable cytochrome-c reduction. Each of these assays allowed correct identification of 5 CGD patients among 30 studied, although the NBT test failed to recognize a low production of O2 - in a patient then found affected from a variant form of the disease. These three diagnostic assays were then used to study anion superoxide release in neutrophils derived from 7 mothers or sisters of X-CGD patients. Interestingly, in one of the CGD carriers we found that about 6% of DHR-loaded granulocytes failed to respond to PMA stimulation, thus indicating that this subject was an X-CGD carrier as confirmed by mutational analysis of CYBB gene. In contrast, the both cytochrome- c reduction test and the NBT test failed to detect the carrier state in this subject. On the basis of our results we conclude that flow cytometric analysis of anion superoxide production by PMN, is an effective, rapid and accurate tool for diagnosis of Chronic Granulomatous Disease and allows precise identification of X-CGD-carrier.
KW - Anion superoxide
KW - Chronic Granulomatous Disease
KW - Diagnosis, flow citometry
KW - Neutrophils
UR - http://www.scopus.com/inward/record.url?scp=0012178771&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0012178771&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0012178771
VL - 23
SP - 1029
EP - 1035
JO - Rivista Italiana di Pediatria
JF - Rivista Italiana di Pediatria
SN - 0392-5161
IS - 6
ER -