Chronic myeloid leukaemia lymphoid blast crisis. Relevance of molecular analysis at the bcr and immunoglobulin heavy chain gene level in monitoring response to therapy and residual disease

F. Lo Coco, D. Diverio, M. Frontani, Y. Z. Wang, E. Montefusco, P. De Fabritiis, W. Arcese, G. De Rossi, F. Mandelli

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Southern blot analyses were performed in sequential DNA samples from 4 patients with Ph'+ chronic myeloid leukaemia (CML) who underwent lymphoid or mixed blast crisis (BC). Genomic rearrangements at the breakpoint cluster region (bcr) and immunoglobulin heavy chain (IgH) gene level provided, in these cases, a sensitive and specific evaluation of response to therapy both in terms of blasts and Ph'+ cell suppression. Recurrent BC was molecularly characterized in the 4 patients, showing each time identical individual specific DNA rearrangement patterns. Residual blasts were detected in 2 cases during intervening chronic phases by IgH rearrangements. Such findings highlight the specificity of these molecular markers, clearly indicating the failure of ablative therapy in eradicating the neoplastic clone. Finally, molecular and phenotypic identity in individual recurrent BC also suggested, in our cases, a lack of clonal evolution during disease progression.

Original languageEnglish
Pages (from-to)172-176
Number of pages5
JournalEuropean Journal of Haematology
Volume46
Issue number3
Publication statusPublished - 1991

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Immunoglobulin Heavy Chain Genes
Blast Crisis
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Clonal Evolution
Immunoglobulin Heavy Chains
Gene Rearrangement
Southern Blotting
Disease Progression
Therapeutics
Clone Cells
DNA

ASJC Scopus subject areas

  • Hematology

Cite this

Chronic myeloid leukaemia lymphoid blast crisis. Relevance of molecular analysis at the bcr and immunoglobulin heavy chain gene level in monitoring response to therapy and residual disease. / Lo Coco, F.; Diverio, D.; Frontani, M.; Wang, Y. Z.; Montefusco, E.; De Fabritiis, P.; Arcese, W.; De Rossi, G.; Mandelli, F.

In: European Journal of Haematology, Vol. 46, No. 3, 1991, p. 172-176.

Research output: Contribution to journalArticle

Lo Coco, F. ; Diverio, D. ; Frontani, M. ; Wang, Y. Z. ; Montefusco, E. ; De Fabritiis, P. ; Arcese, W. ; De Rossi, G. ; Mandelli, F. / Chronic myeloid leukaemia lymphoid blast crisis. Relevance of molecular analysis at the bcr and immunoglobulin heavy chain gene level in monitoring response to therapy and residual disease. In: European Journal of Haematology. 1991 ; Vol. 46, No. 3. pp. 172-176.
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abstract = "Southern blot analyses were performed in sequential DNA samples from 4 patients with Ph'+ chronic myeloid leukaemia (CML) who underwent lymphoid or mixed blast crisis (BC). Genomic rearrangements at the breakpoint cluster region (bcr) and immunoglobulin heavy chain (IgH) gene level provided, in these cases, a sensitive and specific evaluation of response to therapy both in terms of blasts and Ph'+ cell suppression. Recurrent BC was molecularly characterized in the 4 patients, showing each time identical individual specific DNA rearrangement patterns. Residual blasts were detected in 2 cases during intervening chronic phases by IgH rearrangements. Such findings highlight the specificity of these molecular markers, clearly indicating the failure of ablative therapy in eradicating the neoplastic clone. Finally, molecular and phenotypic identity in individual recurrent BC also suggested, in our cases, a lack of clonal evolution during disease progression.",
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AU - Frontani, M.

AU - Wang, Y. Z.

AU - Montefusco, E.

AU - De Fabritiis, P.

AU - Arcese, W.

AU - De Rossi, G.

AU - Mandelli, F.

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