Circadian variations in expression of the trkB receptor in adult rat hippocampus

Claudia Dolci, Angela Montaruli, Eliana Roveda, Isabella Barajon, Laura Vizzotto, Gigliola Grassi Zucconi, Franca Carandente

Research output: Contribution to journalArticle

Abstract

The expression of brain-derived neurotrophic factor (BDNF) in the central nervous system (CNS) and the expression of its high-affinity trkB receptor on neuron surfaces are known to depend on neuron activity. The expression of BDNF (mRNA and protein) and trkB mRNA shows circadian oscillations in rat hippocampal homogenates. We investigated circadian variations in trkB expression in specific areas of the adult rat hippocampal formation by immunohistochemistry. In sets of two experiments performed in the spring, 39 2-month-old male Wistar rats were accustomed to a 12-h light-12-h dark cycle for 2 weeks. Three animals were then sacrificed every 4 h. Forty-micrometer- thick coronal sections of hippocampal formation were obtained and processed for trkB immunohistochemistry. Cell staining intensity was assessed by image analysis of different hippocampal areas on five sections per animal. Circadian rhythmicity was evaluated by the cosinor method. Statistically significant circadian variations in trkB expression were found in dentate gyrus, entorhinal cortex, and the CA3 and hilar regions of the hippocampus, with highest expression during the first half of the dark (activity) period. These findings suggest a relationship between trkB expression and the physiological neuronal activation of wakefulness. TrkB receptor expression in the hippocampal regions studied was continuous and changes were gradual over the 24-h cycle, suggesting that more complex regulatory mechanisms also intervened.

Original languageEnglish
Pages (from-to)67-72
Number of pages6
JournalBrain Research
Volume994
Issue number1
DOIs
Publication statusPublished - Dec 19 2003

Fingerprint

trkB Receptor
Hippocampus
Brain-Derived Neurotrophic Factor
Immunohistochemistry
Neurons
Messenger RNA
Entorhinal Cortex
Wakefulness
Dentate Gyrus
Nerve Growth Factors
Periodicity
Wistar Rats
Central Nervous System
Staining and Labeling
Light

Keywords

  • Circadian variations
  • Hippocampus
  • Rat
  • trkB receptor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Dolci, C., Montaruli, A., Roveda, E., Barajon, I., Vizzotto, L., Zucconi, G. G., & Carandente, F. (2003). Circadian variations in expression of the trkB receptor in adult rat hippocampus. Brain Research, 994(1), 67-72. https://doi.org/10.1016/j.brainres.2003.09.018

Circadian variations in expression of the trkB receptor in adult rat hippocampus. / Dolci, Claudia; Montaruli, Angela; Roveda, Eliana; Barajon, Isabella; Vizzotto, Laura; Zucconi, Gigliola Grassi; Carandente, Franca.

In: Brain Research, Vol. 994, No. 1, 19.12.2003, p. 67-72.

Research output: Contribution to journalArticle

Dolci, C, Montaruli, A, Roveda, E, Barajon, I, Vizzotto, L, Zucconi, GG & Carandente, F 2003, 'Circadian variations in expression of the trkB receptor in adult rat hippocampus', Brain Research, vol. 994, no. 1, pp. 67-72. https://doi.org/10.1016/j.brainres.2003.09.018
Dolci, Claudia ; Montaruli, Angela ; Roveda, Eliana ; Barajon, Isabella ; Vizzotto, Laura ; Zucconi, Gigliola Grassi ; Carandente, Franca. / Circadian variations in expression of the trkB receptor in adult rat hippocampus. In: Brain Research. 2003 ; Vol. 994, No. 1. pp. 67-72.
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