Circadian variations of autologous mixed lymphocyte reactions and endogenous cortisol

F. Indiveri, I. Pierri, S. Rogna, A. Poggi, P. Montaldo, R. Romano, A. Pende, A. Morgano, A. Barabino, S. Ferrone

Research output: Contribution to journalArticlepeer-review

Abstract

The degree of proliferation of human T cells stimulated with autologous PHA-T cells and with autologous non-T cells displays circadian variations. The highest proliferation occurs with cells isolated from blood drawn at 8 a.m. in mixed lymphocyte reactions (MLR) with autologous PHA-T cells and from blood drawn at 8 p.m. in MLR with autologous non-T cells. The circadian variations of autologous MLRs appear to reflect changes in the proliferative response of T cells. In autologous MLRs with non-T cells as stimulators the extent of proliferation was inversely correlated with the level of endogenous cortisol. The circadian variations of autologous MLRs do not reflect non-specific changes in the proliferative and stimulatory properties of T and non-T cells, since circadian variations were not observed in the proliferative response of T cells to mitogens and in allogeneic MLRs. Circadian variations of autologous MLRs must be taken into account when analyzing abnormalities of these reactions in pathological conditions.

Original languageEnglish
Pages (from-to)17-24
Number of pages8
JournalJournal of Immunological Methods
Volume82
Issue number1
DOIs
Publication statusPublished - Sep 3 1985

Keywords

  • autologous MLR
  • autologous MLR, circadian variations
  • Class II HLA antigens
  • endogenous cortisol

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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