Circulating antibodies recognizing oxidatively modified low-density lipoprotein in children

Lorenzo Iughetti, Cecilia Volta, Elena Maggi, Giuseppina Palladini, Cristina Perugini, Giorgio Bellomo, Sergio Bernasconi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The occurrence of circulating antibodies (IgG) against oxidatively modified LDL was investigated in a group of normocholesterolemic, healthy, cardiovascular risk-free children and in a comparable group of normal adults. An increased titer of IgG recognizing Cu++-oxidized or malondialdehyde (MDA)-derivatized LDL (MDA-LDL) was a constant feature in children. The antigenic epitopes recognized by these antibodies were generated rather late in the process of copper-mediated LDL oxidation, concomitantly with the formation of fluorescent adducts between reactive aldehydes (including MDA) and apo B100. MDA-LDL was not the only antigen recognized: derivatization of other structurally unrelated proteins, such as HDL, serum albumin, fibrinogen, and transferrin with MDA led to generation of recognizable epitopes. However, among the various modified proteins, LDL exhibited the highest binding activity for IgG present in the group of children. This was associated with an enhanced propensity of LDL isolated from children to undergo in vitro oxidation, despite normal levels of the endogenous antioxidant α-tocopherol. These findings indicate that circulating antibodies recognizing proteins modified with end-products of lipid peroxidation (including LDL) are present in healthy cardiovascular risk-free children. The possibility that LDL oxidation occurs in vivo already in childhood and may act as the real immunogen is an attractive but still unproven hypothesis.

Original languageEnglish
Pages (from-to)94-99
Number of pages6
JournalPediatric Research
Volume45
Issue number1
Publication statusPublished - Jan 1999

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LDL Lipoproteins
Antibodies
Malondialdehyde
Immunoglobulin G
Epitopes
Proteins
Tocopherols
Transferrin
oxidized low density lipoprotein
Aldehydes
Serum Albumin
Fibrinogen
Lipid Peroxidation
Copper
Antioxidants
Antigens

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Iughetti, L., Volta, C., Maggi, E., Palladini, G., Perugini, C., Bellomo, G., & Bernasconi, S. (1999). Circulating antibodies recognizing oxidatively modified low-density lipoprotein in children. Pediatric Research, 45(1), 94-99.

Circulating antibodies recognizing oxidatively modified low-density lipoprotein in children. / Iughetti, Lorenzo; Volta, Cecilia; Maggi, Elena; Palladini, Giuseppina; Perugini, Cristina; Bellomo, Giorgio; Bernasconi, Sergio.

In: Pediatric Research, Vol. 45, No. 1, 01.1999, p. 94-99.

Research output: Contribution to journalArticle

Iughetti, L, Volta, C, Maggi, E, Palladini, G, Perugini, C, Bellomo, G & Bernasconi, S 1999, 'Circulating antibodies recognizing oxidatively modified low-density lipoprotein in children', Pediatric Research, vol. 45, no. 1, pp. 94-99.
Iughetti L, Volta C, Maggi E, Palladini G, Perugini C, Bellomo G et al. Circulating antibodies recognizing oxidatively modified low-density lipoprotein in children. Pediatric Research. 1999 Jan;45(1):94-99.
Iughetti, Lorenzo ; Volta, Cecilia ; Maggi, Elena ; Palladini, Giuseppina ; Perugini, Cristina ; Bellomo, Giorgio ; Bernasconi, Sergio. / Circulating antibodies recognizing oxidatively modified low-density lipoprotein in children. In: Pediatric Research. 1999 ; Vol. 45, No. 1. pp. 94-99.
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