Circulating CD14+ and CD14highCD16- classical monocytes are reduced in patients with signs of plaque neovascularization in the carotid artery

Enrico Ammirati, Francesco Moroni, Marco Magnoni, Simona Di Terlizzi, Chiara Maria Villa, Federico Sizzano, Alessio Palini, Katia Garlaschelli, Fernanda Tripiciano, Isabella Scotti, Alberico Luigi Catapano, Angelo Andrea Manfredi, Giuseppe Danilo Norata, Paolo Camici

Research output: Contribution to journalArticle

Abstract

Background and aims: Monocytes are known to play a key role in the initiation and progression of atherosclerosis and contribute to plaque destabilization through the generation of signals that promote inflammation and neoangiogenesis. In humans, studies investigating the features of circulating monocytes in advanced atherosclerotic lesions are lacking. Methods: Patients (mean age 69 years, 56% males) with intermediate asymptomatic carotid stenosis (40-70% in diameter) were evaluated for maximal stenosis in common carotid artery, carotid bulb and internal carotid artery, overall disease burden as estimated with total plaque area (TPA), greyscale and neovascularization in 244 advanced carotid plaques. Absolute counts of circulating CD14+ monocytes, of classical (CD14highCD16-), intermediate (CD14highCD16+) and non-classical (CD14lowCD16+) monocytes and HLA-DR+ median fluorescence intensity for each subset were evaluated with flow cytometry. Results: No correlation was found between monocytes and overall atherosclerotic burden, nor with high sensitivity C-reactive protein (hsCRP) or interleukin-6 (IL-6). In contrast, plaque signs of neovascularization were associated with significantly lower counts of circulating CD14+ monocytes (297 versus 350 cells/mm3, p = 0.039) and of classical monocytes (255 versus 310 cells/mm3, p = 0.029). Conclusions: Neovascularized atherosclerotic lesions selectively associate with lower blood levels of CD14+ and CD14highCD16- monocytes independently of systemic inflammatory activity, as indicated by normal hsCRP levels. Whether the reduction of circulating CD14+ and CD14highCD16- monocytes is due to a potential redistribution of these cell types into active lesions remains to be explored.

Original languageEnglish
JournalAtherosclerosis
DOIs
Publication statusAccepted/In press - Jan 16 2016

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Carotid Arteries
Monocytes
Carotid Stenosis
C-Reactive Protein
Carotid Artery Diseases
HLA-DR Antigens
Interleukin-6
Atherosclerosis
Flow Cytometry
Fluorescence
Inflammation

Keywords

  • Carotid atherosclerosis
  • Classical monocytes
  • Contrast enhanced ultrasound
  • Monocytes subsets
  • Plaque neovascularization

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Circulating CD14+ and CD14highCD16- classical monocytes are reduced in patients with signs of plaque neovascularization in the carotid artery. / Ammirati, Enrico; Moroni, Francesco; Magnoni, Marco; Di Terlizzi, Simona; Villa, Chiara Maria; Sizzano, Federico; Palini, Alessio; Garlaschelli, Katia; Tripiciano, Fernanda; Scotti, Isabella; Catapano, Alberico Luigi; Manfredi, Angelo Andrea; Norata, Giuseppe Danilo; Camici, Paolo.

In: Atherosclerosis, 16.01.2016.

Research output: Contribution to journalArticle

Ammirati, Enrico ; Moroni, Francesco ; Magnoni, Marco ; Di Terlizzi, Simona ; Villa, Chiara Maria ; Sizzano, Federico ; Palini, Alessio ; Garlaschelli, Katia ; Tripiciano, Fernanda ; Scotti, Isabella ; Catapano, Alberico Luigi ; Manfredi, Angelo Andrea ; Norata, Giuseppe Danilo ; Camici, Paolo. / Circulating CD14+ and CD14highCD16- classical monocytes are reduced in patients with signs of plaque neovascularization in the carotid artery. In: Atherosclerosis. 2016.
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abstract = "Background and aims: Monocytes are known to play a key role in the initiation and progression of atherosclerosis and contribute to plaque destabilization through the generation of signals that promote inflammation and neoangiogenesis. In humans, studies investigating the features of circulating monocytes in advanced atherosclerotic lesions are lacking. Methods: Patients (mean age 69 years, 56{\%} males) with intermediate asymptomatic carotid stenosis (40-70{\%} in diameter) were evaluated for maximal stenosis in common carotid artery, carotid bulb and internal carotid artery, overall disease burden as estimated with total plaque area (TPA), greyscale and neovascularization in 244 advanced carotid plaques. Absolute counts of circulating CD14+ monocytes, of classical (CD14highCD16-), intermediate (CD14highCD16+) and non-classical (CD14lowCD16+) monocytes and HLA-DR+ median fluorescence intensity for each subset were evaluated with flow cytometry. Results: No correlation was found between monocytes and overall atherosclerotic burden, nor with high sensitivity C-reactive protein (hsCRP) or interleukin-6 (IL-6). In contrast, plaque signs of neovascularization were associated with significantly lower counts of circulating CD14+ monocytes (297 versus 350 cells/mm3, p = 0.039) and of classical monocytes (255 versus 310 cells/mm3, p = 0.029). Conclusions: Neovascularized atherosclerotic lesions selectively associate with lower blood levels of CD14+ and CD14highCD16- monocytes independently of systemic inflammatory activity, as indicated by normal hsCRP levels. Whether the reduction of circulating CD14+ and CD14highCD16- monocytes is due to a potential redistribution of these cell types into active lesions remains to be explored.",
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author = "Enrico Ammirati and Francesco Moroni and Marco Magnoni and {Di Terlizzi}, Simona and Villa, {Chiara Maria} and Federico Sizzano and Alessio Palini and Katia Garlaschelli and Fernanda Tripiciano and Isabella Scotti and Catapano, {Alberico Luigi} and Manfredi, {Angelo Andrea} and Norata, {Giuseppe Danilo} and Paolo Camici",
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T1 - Circulating CD14+ and CD14highCD16- classical monocytes are reduced in patients with signs of plaque neovascularization in the carotid artery

AU - Ammirati, Enrico

AU - Moroni, Francesco

AU - Magnoni, Marco

AU - Di Terlizzi, Simona

AU - Villa, Chiara Maria

AU - Sizzano, Federico

AU - Palini, Alessio

AU - Garlaschelli, Katia

AU - Tripiciano, Fernanda

AU - Scotti, Isabella

AU - Catapano, Alberico Luigi

AU - Manfredi, Angelo Andrea

AU - Norata, Giuseppe Danilo

AU - Camici, Paolo

PY - 2016/1/16

Y1 - 2016/1/16

N2 - Background and aims: Monocytes are known to play a key role in the initiation and progression of atherosclerosis and contribute to plaque destabilization through the generation of signals that promote inflammation and neoangiogenesis. In humans, studies investigating the features of circulating monocytes in advanced atherosclerotic lesions are lacking. Methods: Patients (mean age 69 years, 56% males) with intermediate asymptomatic carotid stenosis (40-70% in diameter) were evaluated for maximal stenosis in common carotid artery, carotid bulb and internal carotid artery, overall disease burden as estimated with total plaque area (TPA), greyscale and neovascularization in 244 advanced carotid plaques. Absolute counts of circulating CD14+ monocytes, of classical (CD14highCD16-), intermediate (CD14highCD16+) and non-classical (CD14lowCD16+) monocytes and HLA-DR+ median fluorescence intensity for each subset were evaluated with flow cytometry. Results: No correlation was found between monocytes and overall atherosclerotic burden, nor with high sensitivity C-reactive protein (hsCRP) or interleukin-6 (IL-6). In contrast, plaque signs of neovascularization were associated with significantly lower counts of circulating CD14+ monocytes (297 versus 350 cells/mm3, p = 0.039) and of classical monocytes (255 versus 310 cells/mm3, p = 0.029). Conclusions: Neovascularized atherosclerotic lesions selectively associate with lower blood levels of CD14+ and CD14highCD16- monocytes independently of systemic inflammatory activity, as indicated by normal hsCRP levels. Whether the reduction of circulating CD14+ and CD14highCD16- monocytes is due to a potential redistribution of these cell types into active lesions remains to be explored.

AB - Background and aims: Monocytes are known to play a key role in the initiation and progression of atherosclerosis and contribute to plaque destabilization through the generation of signals that promote inflammation and neoangiogenesis. In humans, studies investigating the features of circulating monocytes in advanced atherosclerotic lesions are lacking. Methods: Patients (mean age 69 years, 56% males) with intermediate asymptomatic carotid stenosis (40-70% in diameter) were evaluated for maximal stenosis in common carotid artery, carotid bulb and internal carotid artery, overall disease burden as estimated with total plaque area (TPA), greyscale and neovascularization in 244 advanced carotid plaques. Absolute counts of circulating CD14+ monocytes, of classical (CD14highCD16-), intermediate (CD14highCD16+) and non-classical (CD14lowCD16+) monocytes and HLA-DR+ median fluorescence intensity for each subset were evaluated with flow cytometry. Results: No correlation was found between monocytes and overall atherosclerotic burden, nor with high sensitivity C-reactive protein (hsCRP) or interleukin-6 (IL-6). In contrast, plaque signs of neovascularization were associated with significantly lower counts of circulating CD14+ monocytes (297 versus 350 cells/mm3, p = 0.039) and of classical monocytes (255 versus 310 cells/mm3, p = 0.029). Conclusions: Neovascularized atherosclerotic lesions selectively associate with lower blood levels of CD14+ and CD14highCD16- monocytes independently of systemic inflammatory activity, as indicated by normal hsCRP levels. Whether the reduction of circulating CD14+ and CD14highCD16- monocytes is due to a potential redistribution of these cell types into active lesions remains to be explored.

KW - Carotid atherosclerosis

KW - Classical monocytes

KW - Contrast enhanced ultrasound

KW - Monocytes subsets

KW - Plaque neovascularization

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U2 - 10.1016/j.atherosclerosis.2016.10.004

DO - 10.1016/j.atherosclerosis.2016.10.004

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JO - Atherosclerosis

JF - Atherosclerosis

SN - 0021-9150

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