Circulating GH isoforms and GH bioactivity in preterm neonates

Giorgio Radetti, Mauro Bozzola, Sara Pagani, Maria Antonietta Avanzini, Hubert Messner, Cesare Belloni, Masakane Hayakawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Among the molecular variants of human GH, the monomeric 22-kD is the predominant isoform, whereas the 20-kD is the second most abundant isoform. Because little is known on the pattern of human GH isoforms in the early postnatal period, we evaluated serum levels of 22-kD GH by an immunofluorometric assay and of 20-kD GH by an ELISA using an anti-20-kD antibody, and measured GH bioactivity with the Nb2 cell bioassay in 19 preterm neonates (gestational age, 32 ± 0.5 wk; mean ± SEM) on the fourth and 15th days of life. As control subjects, we studied 19 full-term neonates (gestational age, 39 ± 0.3 wk) on the fourth day of life and 20 healthy adults, aged 20 ± 0.3 y. Four-day-old preterm neonates showed significantly higher serum values Of 20-kD GH (0.99 ± 0.14 ng/mL) than full-term neonates (0.33 ± 0.07 ng/mL; p <0.001) and adults (0.09 ± 0.02; p <0.0001). Likewise, 22-kD GH and GH levels by Nb2 cell bioassay were also significantly higher (p <0.001) in preterm than in full-term neonates and young adults. A significant decrease (p <0.01) in 20-kD, 22-kD, and Nb2-determined GH was observed in preterm neonates on the 15th day of life The percentage of the 20-kD isoform was similar in the preterm infants at the fourth and 15th day, in full-term infants, and in adults (2.7%, 2.7%, 2.8%, and 3.16%, respectively). Our results indicate that 20-kD GH serum levels change throughout life as regards total amount, but not as regards percentage.

Original languageEnglish
Pages (from-to)244-247
Number of pages4
JournalPediatric Research
Volume48
Issue number2
Publication statusPublished - 2000

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Protein Isoforms
Newborn Infant
Biological Assay
Gestational Age
Serum
Fluoroimmunoassay
Premature Infants
Young Adult
Enzyme-Linked Immunosorbent Assay
Antibodies

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Radetti, G., Bozzola, M., Pagani, S., Avanzini, M. A., Messner, H., Belloni, C., & Hayakawa, M. (2000). Circulating GH isoforms and GH bioactivity in preterm neonates. Pediatric Research, 48(2), 244-247.

Circulating GH isoforms and GH bioactivity in preterm neonates. / Radetti, Giorgio; Bozzola, Mauro; Pagani, Sara; Avanzini, Maria Antonietta; Messner, Hubert; Belloni, Cesare; Hayakawa, Masakane.

In: Pediatric Research, Vol. 48, No. 2, 2000, p. 244-247.

Research output: Contribution to journalArticle

Radetti, G, Bozzola, M, Pagani, S, Avanzini, MA, Messner, H, Belloni, C & Hayakawa, M 2000, 'Circulating GH isoforms and GH bioactivity in preterm neonates', Pediatric Research, vol. 48, no. 2, pp. 244-247.
Radetti G, Bozzola M, Pagani S, Avanzini MA, Messner H, Belloni C et al. Circulating GH isoforms and GH bioactivity in preterm neonates. Pediatric Research. 2000;48(2):244-247.
Radetti, Giorgio ; Bozzola, Mauro ; Pagani, Sara ; Avanzini, Maria Antonietta ; Messner, Hubert ; Belloni, Cesare ; Hayakawa, Masakane. / Circulating GH isoforms and GH bioactivity in preterm neonates. In: Pediatric Research. 2000 ; Vol. 48, No. 2. pp. 244-247.
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