Circulating immune complexes in allogeneic marrow graft recipients

Fabrizio Manca, Andrea Bacigalupo, Maria Teresa Van Lint, Giorgio Trovatello, Sebastiana Cantarella, Francesco Frassoni, Alberto Marmont, Franco Celada

Research output: Contribution to journalArticle

Abstract

Human monocytes (M–) exposed to 0.5–20 ug/ml of cyclosporine (CsA) produced levels of prostaglandins of the E series (PGE) that were 2–3-fold greater than control M– cultured in medium alone. Maximal PGE levels were obtained at 24–48 hr incubation, and the failure to observe a linear increase of PGE levels at higher CsA concentrations appeared partially related to cytotoxic effects. CsA was considerably less effective than phorbol myristate acetate or bacterial lipopolysac-charide in increasing PGE production, but the PGE levels achieved with CsA approximated those known to suppress immune responsiveness. Other experiments showed that, although the increased PGE production with CsA was indomethacin-sensitive, CsA mostly functioned to increase the availability of free arachidonic acid (AA) instead of accelerating AA conversion by the cyclooxygenase pathway. Thus CsA can alter M– physiology, and these alterations might inhibit quite early events during the induction phase of immune responses.

Original languageEnglish
Pages (from-to)377-381
Number of pages5
JournalTransplantation
Volume38
Issue number4
Publication statusPublished - 1984

Fingerprint

Prostaglandins E
Antigen-Antibody Complex
Bone Marrow
Transplants
Tetradecanoylphorbol Acetate
Prostaglandin-Endoperoxide Synthases
Arachidonic Acid
Indomethacin
Cyclosporine
Monocytes

ASJC Scopus subject areas

  • Transplantation

Cite this

Manca, F., Bacigalupo, A., Van Lint, M. T., Trovatello, G., Cantarella, S., Frassoni, F., ... Celada, F. (1984). Circulating immune complexes in allogeneic marrow graft recipients. Transplantation, 38(4), 377-381.

Circulating immune complexes in allogeneic marrow graft recipients. / Manca, Fabrizio; Bacigalupo, Andrea; Van Lint, Maria Teresa; Trovatello, Giorgio; Cantarella, Sebastiana; Frassoni, Francesco; Marmont, Alberto; Celada, Franco.

In: Transplantation, Vol. 38, No. 4, 1984, p. 377-381.

Research output: Contribution to journalArticle

Manca, F, Bacigalupo, A, Van Lint, MT, Trovatello, G, Cantarella, S, Frassoni, F, Marmont, A & Celada, F 1984, 'Circulating immune complexes in allogeneic marrow graft recipients', Transplantation, vol. 38, no. 4, pp. 377-381.
Manca F, Bacigalupo A, Van Lint MT, Trovatello G, Cantarella S, Frassoni F et al. Circulating immune complexes in allogeneic marrow graft recipients. Transplantation. 1984;38(4):377-381.
Manca, Fabrizio ; Bacigalupo, Andrea ; Van Lint, Maria Teresa ; Trovatello, Giorgio ; Cantarella, Sebastiana ; Frassoni, Francesco ; Marmont, Alberto ; Celada, Franco. / Circulating immune complexes in allogeneic marrow graft recipients. In: Transplantation. 1984 ; Vol. 38, No. 4. pp. 377-381.
@article{14def242fe3440ac9fd088445a7e341d,
title = "Circulating immune complexes in allogeneic marrow graft recipients",
abstract = "Human monocytes (M–) exposed to 0.5–20 ug/ml of cyclosporine (CsA) produced levels of prostaglandins of the E series (PGE) that were 2–3-fold greater than control M– cultured in medium alone. Maximal PGE levels were obtained at 24–48 hr incubation, and the failure to observe a linear increase of PGE levels at higher CsA concentrations appeared partially related to cytotoxic effects. CsA was considerably less effective than phorbol myristate acetate or bacterial lipopolysac-charide in increasing PGE production, but the PGE levels achieved with CsA approximated those known to suppress immune responsiveness. Other experiments showed that, although the increased PGE production with CsA was indomethacin-sensitive, CsA mostly functioned to increase the availability of free arachidonic acid (AA) instead of accelerating AA conversion by the cyclooxygenase pathway. Thus CsA can alter M– physiology, and these alterations might inhibit quite early events during the induction phase of immune responses.",
author = "Fabrizio Manca and Andrea Bacigalupo and {Van Lint}, {Maria Teresa} and Giorgio Trovatello and Sebastiana Cantarella and Francesco Frassoni and Alberto Marmont and Franco Celada",
year = "1984",
language = "English",
volume = "38",
pages = "377--381",
journal = "Transplantation",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Circulating immune complexes in allogeneic marrow graft recipients

AU - Manca, Fabrizio

AU - Bacigalupo, Andrea

AU - Van Lint, Maria Teresa

AU - Trovatello, Giorgio

AU - Cantarella, Sebastiana

AU - Frassoni, Francesco

AU - Marmont, Alberto

AU - Celada, Franco

PY - 1984

Y1 - 1984

N2 - Human monocytes (M–) exposed to 0.5–20 ug/ml of cyclosporine (CsA) produced levels of prostaglandins of the E series (PGE) that were 2–3-fold greater than control M– cultured in medium alone. Maximal PGE levels were obtained at 24–48 hr incubation, and the failure to observe a linear increase of PGE levels at higher CsA concentrations appeared partially related to cytotoxic effects. CsA was considerably less effective than phorbol myristate acetate or bacterial lipopolysac-charide in increasing PGE production, but the PGE levels achieved with CsA approximated those known to suppress immune responsiveness. Other experiments showed that, although the increased PGE production with CsA was indomethacin-sensitive, CsA mostly functioned to increase the availability of free arachidonic acid (AA) instead of accelerating AA conversion by the cyclooxygenase pathway. Thus CsA can alter M– physiology, and these alterations might inhibit quite early events during the induction phase of immune responses.

AB - Human monocytes (M–) exposed to 0.5–20 ug/ml of cyclosporine (CsA) produced levels of prostaglandins of the E series (PGE) that were 2–3-fold greater than control M– cultured in medium alone. Maximal PGE levels were obtained at 24–48 hr incubation, and the failure to observe a linear increase of PGE levels at higher CsA concentrations appeared partially related to cytotoxic effects. CsA was considerably less effective than phorbol myristate acetate or bacterial lipopolysac-charide in increasing PGE production, but the PGE levels achieved with CsA approximated those known to suppress immune responsiveness. Other experiments showed that, although the increased PGE production with CsA was indomethacin-sensitive, CsA mostly functioned to increase the availability of free arachidonic acid (AA) instead of accelerating AA conversion by the cyclooxygenase pathway. Thus CsA can alter M– physiology, and these alterations might inhibit quite early events during the induction phase of immune responses.

UR - http://www.scopus.com/inward/record.url?scp=84930544618&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84930544618&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84930544618

VL - 38

SP - 377

EP - 381

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 4

ER -