Circulating immune complexes in Behcet's syndrome

Purification, characterization and cross-reactivity studies

G. Valesini, M. Picardo, R. Pastore, P. Pivetti, U. Serafini

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The C1q-binding assay was performed on 30 sera from patients with Behcet's syndrome and circulating immune complexes were found in 46%. Circulating immune complexes were isolated and purified from the sera of two patients by immunoadsorption on a column of polymethylmetacrylate beads coated with C1q and then labelled with 125I. In double immunodiffusion these purified immune complexes were found to contain IgG, C1q, C1s and C3. Anti-IgG activity was not detectable in the purified immune complexes while specific cross-reactivity was found in a solid-phase radioimmunoassay with the majority of the sera of Behcet's syndrome.

Original languageEnglish
Pages (from-to)522-527
Number of pages6
JournalClinical and Experimental Immunology
Volume44
Issue number3
Publication statusPublished - 1981

Fingerprint

Behcet Syndrome
Antigen-Antibody Complex
Serum
Immunodiffusion
Polymethyl Methacrylate
Radioimmunoassay
Immunoglobulin G

ASJC Scopus subject areas

  • Immunology

Cite this

Circulating immune complexes in Behcet's syndrome : Purification, characterization and cross-reactivity studies. / Valesini, G.; Picardo, M.; Pastore, R.; Pivetti, P.; Serafini, U.

In: Clinical and Experimental Immunology, Vol. 44, No. 3, 1981, p. 522-527.

Research output: Contribution to journalArticle

@article{458a0b53d09a4940b9c594713ddd92ec,
title = "Circulating immune complexes in Behcet's syndrome: Purification, characterization and cross-reactivity studies",
abstract = "The C1q-binding assay was performed on 30 sera from patients with Behcet's syndrome and circulating immune complexes were found in 46{\%}. Circulating immune complexes were isolated and purified from the sera of two patients by immunoadsorption on a column of polymethylmetacrylate beads coated with C1q and then labelled with 125I. In double immunodiffusion these purified immune complexes were found to contain IgG, C1q, C1s and C3. Anti-IgG activity was not detectable in the purified immune complexes while specific cross-reactivity was found in a solid-phase radioimmunoassay with the majority of the sera of Behcet's syndrome.",
author = "G. Valesini and M. Picardo and R. Pastore and P. Pivetti and U. Serafini",
year = "1981",
language = "English",
volume = "44",
pages = "522--527",
journal = "Clinical and Experimental Immunology",
issn = "0009-9104",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Circulating immune complexes in Behcet's syndrome

T2 - Purification, characterization and cross-reactivity studies

AU - Valesini, G.

AU - Picardo, M.

AU - Pastore, R.

AU - Pivetti, P.

AU - Serafini, U.

PY - 1981

Y1 - 1981

N2 - The C1q-binding assay was performed on 30 sera from patients with Behcet's syndrome and circulating immune complexes were found in 46%. Circulating immune complexes were isolated and purified from the sera of two patients by immunoadsorption on a column of polymethylmetacrylate beads coated with C1q and then labelled with 125I. In double immunodiffusion these purified immune complexes were found to contain IgG, C1q, C1s and C3. Anti-IgG activity was not detectable in the purified immune complexes while specific cross-reactivity was found in a solid-phase radioimmunoassay with the majority of the sera of Behcet's syndrome.

AB - The C1q-binding assay was performed on 30 sera from patients with Behcet's syndrome and circulating immune complexes were found in 46%. Circulating immune complexes were isolated and purified from the sera of two patients by immunoadsorption on a column of polymethylmetacrylate beads coated with C1q and then labelled with 125I. In double immunodiffusion these purified immune complexes were found to contain IgG, C1q, C1s and C3. Anti-IgG activity was not detectable in the purified immune complexes while specific cross-reactivity was found in a solid-phase radioimmunoassay with the majority of the sera of Behcet's syndrome.

UR - http://www.scopus.com/inward/record.url?scp=0019467899&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019467899&partnerID=8YFLogxK

M3 - Article

VL - 44

SP - 522

EP - 527

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

SN - 0009-9104

IS - 3

ER -