Circulating levels of tumour necrosis factor-α and its soluble receptors are increased in the blood of patients with amyotrophic lateral sclerosis

Marco Poloni, Dante Facchetti, Roberto Mai, Anna Micheli, Laura Agnoletti, Gloria Francolini, Gabriele Mora, Claudia Camana, Letizia Mazzini, Tiziana Bachetti

Research output: Contribution to journalArticlepeer-review

Abstract

An immunologic pathogenesis for amyotrophic lateral sclerosis (ALS) has been recently proposed. We tested the whole tumour necrosis factor (TNF) system in the serum of 51 ALS patients at different stages of the disease and 36 healthy controls. Antigenic TNF-α and its soluble receptors (sTNF-Rs), measured by ELISA, were significantly higher in ALS patients than in healthy controls. However, biologically active TNF-α, corresponding to the sTNF-Rs-unbound trimeric TNFα molecule and assayed by its cytotoxic activity on a sensitive cell line, was similar between ALS patients and healthy controls. Neither antigenic TNF-α, bioactive TNF-α nor sTNF-Rs correlated with disease severity, disease duration, or weight loss. In conclusion, we reported an activation of the TNF system in ALS. The role of this activation in the pathogenesis of the disease remains elusive. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)211-214
Number of pages4
JournalNeuroscience Letters
Volume287
Issue number3
DOIs
Publication statusPublished - Jun 30 2000

Keywords

  • Amyotrophic lateral sclerosis
  • Cytokine
  • Neurodegenerative disease
  • Soluble tumour necrosis factor receptors
  • Tumour necrosis factor-α

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Circulating levels of tumour necrosis factor-α and its soluble receptors are increased in the blood of patients with amyotrophic lateral sclerosis'. Together they form a unique fingerprint.

Cite this