Circulating microRNA Profiles as Liquid Biopsies for the Characterization and Diagnosis of Fibromyalgia Syndrome

Andrea Masotti, Antonella Baldassarre, Maria Paola Guzzo, Cristina Iannuccelli, Christian Barbato, Manuela Di Franco

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This work was aimed at investigating the circulating microRNA (miRNA) profiles in serum and saliva of patients affected by fibromyalgia syndrome (FM), correlating their expression values with clinical and clinimetric parameters and to suggest a mathematical model for the diagnosis of FM. A number of 14 FM patients and sex- and age-matched controls were enrolled in our study. The expression of a panel of 179 miRNAs was evaluated by qPCR. Statistical analyses were performed in order to obtain a mathematical linear model, which could be employed as a supporting tool in the diagnosis of FM. Bioinformatics analysis on miRNA targets were performed to obtain the relevant biological processes related to FM syndrome and to characterize in details the disease. Six miRNAs were found downregulated in FM patients compared to controls. Five of these miRNAs have been included in a linear predictive model that reached a very high sensitivity (100 %) and a high specificity (83.3 %). Moreover, miR-320b displayed a significant negative correlation (r = −0.608 and p = 0.036) with ZSDS score. Finally, several biological processes related to brain function/development and muscular functions were found potentially implicated in FM syndrome. Our study suggests that the study of circulating miRNA profiles coupled to statistical and bioinformatics analyses is a useful tool to better characterize the FM syndrome and to propose a preliminary model for its diagnosis.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalMolecular Neurobiology
DOIs
Publication statusAccepted/In press - Oct 29 2016

Fingerprint

Fibromyalgia
MicroRNAs
Biopsy
Biological Phenomena
Computational Biology
Linear Models
Theoretical Models
Muscle Development
Saliva
Down-Regulation

Keywords

  • Biomarkers
  • Circulating miRNAs
  • Depression
  • Fibromyalgia syndrome
  • Liquid biopsies
  • Pain

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Circulating microRNA Profiles as Liquid Biopsies for the Characterization and Diagnosis of Fibromyalgia Syndrome. / Masotti, Andrea; Baldassarre, Antonella; Guzzo, Maria Paola; Iannuccelli, Cristina; Barbato, Christian; Di Franco, Manuela.

In: Molecular Neurobiology, 29.10.2016, p. 1-8.

Research output: Contribution to journalArticle

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