Circulating Micrornas Predict Survival of Patients with Tumors of Glial Origin

A. Drusco, P. Fadda, G. Nigita, M. Fassan, A. Bottoni, Gardiman MP, D. Sacchi, F. Calore, M. Carosi, A. Antenucci, B. Casini, H. Kelani, E. Pescarmona, Di Leva G, N. Zanesi, Berger MS, Croce CM

Research output: Contribution to journalArticle

Abstract

The World Health Organization has recently introduced molecular prognostic-diagnostic biomarkers in the classification of Central Nervous System (CNS) tumors. In order to characterize subclasses of tumors that cannot find a precise location in the current classification, and, or cannot be tested because of scant material, it is important to find new molecular biomarkers in tissue and, or biological fluid samples. In this study, we identified serum microRNAs that could serve as biomarkers for the diagnosis and prognosis of patients with tumors of glial origin. We retrospectively analyzed microRNA expression in the serum extracellular vesicles of patients with tumors of glial origin. Extracellular vesicles RNA was analyzed by Nanostring. qRT-PCR confirmed 6 overexpressed microRNAs: hsa-miR-4443, hsa-miR-422a, hsa-miR-494-3p, hsa-miR-502-5p, hsa-miR-520f-3p, and hsa-miR-549a. Hsa-miR-4443 was the only microRNA that showed significant differences in most comparisons. In situ hybridization (ISH), confirmed that our signature was mostly expressed in cancer cells. Importantly, hsa-miR-549a and hsa-miR-502-5p expression predicted prognosis in patients with tumors of glial origin. Although more studies are needed, we demonstrated that serum vesicles microRNA profiles are promising diagnostic and prognostic molecular biomarkers that will find an actual application in the clinical practice of CNS tumors.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalEBioMedicine
Volume30
DOIs
Publication statusPublished - Apr 1 2018

Keywords

  • Biomarkers
  • CNS tumors
  • Diagnosis
  • microRNA
  • Prognosis
  • Serum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Drusco, A., Fadda, P., Nigita, G., Fassan, M., Bottoni, A., MP, G., Sacchi, D., Calore, F., Carosi, M., Antenucci, A., Casini, B., Kelani, H., Pescarmona, E., G, D. L., Zanesi, N., MS, B., & CM, C. (2018). Circulating Micrornas Predict Survival of Patients with Tumors of Glial Origin. EBioMedicine, 30, 105-112. https://doi.org/10.1016/j.ebiom.2018.03.022