Circulating miR-127-3p as a Potential Biomarker for Differential Diagnosis in Frontotemporal Dementia

Paola Piscopo, Margherita Grasso, Maria Puopolo, Emanuela D'Acunto, Giuseppina Talarico, Alessio Crestini, Marina Gasparini, Rosa Campopiano, Stefano Gambardella, Anna Elisa Castellano, Giuseppe Bruno, Michela A Denti, Annamaria Confaloni

Research output: Contribution to journalArticle

Abstract

Given the heterogeneous nature of frontotemporal dementia (FTD), sensitive biomarkers are greatly needed for the accurate diagnosis of this neurodegenerative disorder. Circulating miRNAs have been reported as promising biomarkers for neurodegenerative disorders and processes affecting the central nervous system, especially in aging. The objective of the study was to evaluate if some circulating miRNAs linked with apoptosis (miR-29b-3p, miR-34a-5p, miR-16-5p, miR-17-5p, miR-107, miR-19b-3p, let-7b-5p, miR-26b-5p, and 127-3p) were able to distinguish between FTD patients and healthy controls. For this study, we enrolled 127 subjects, including 54 patients with FTD, 20 patients with Alzheimer's disease (AD), and 53 healthy controls. The qRT-PCR analysis showed a downregulation of miR-127-3p in FTD compared to controls, while the levels of other miRNAs remained unchanged. Then, miR-127-3p expression was also analyzed in AD patients, finding a different expression between two patient groups. A receiver operating characteristic curve was then created for miR-127-3p to discriminate FTD versus AD (AUC: 0.8986), and versus healthy controls (AUC: 0.8057). In conclusion, miR-127-3p could help to diagnose FTD and to distinguish it from AD.

Original languageEnglish
Pages (from-to)455-464
Number of pages10
JournalJournal of Alzheimer's disease : JAD
Volume65
Issue number2
DOIs
Publication statusPublished - 2018

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Frontotemporal Dementia
Differential Diagnosis
Biomarkers
Alzheimer Disease
MicroRNAs
Neurodegenerative Diseases
Area Under Curve
ROC Curve
Down-Regulation
Central Nervous System
Apoptosis
Polymerase Chain Reaction

Keywords

  • Biomarker
  • differential diagnosis
  • Frontotemporal dementia
  • miR-127-3p
  • miRNA

Cite this

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title = "Circulating miR-127-3p as a Potential Biomarker for Differential Diagnosis in Frontotemporal Dementia",
abstract = "Given the heterogeneous nature of frontotemporal dementia (FTD), sensitive biomarkers are greatly needed for the accurate diagnosis of this neurodegenerative disorder. Circulating miRNAs have been reported as promising biomarkers for neurodegenerative disorders and processes affecting the central nervous system, especially in aging. The objective of the study was to evaluate if some circulating miRNAs linked with apoptosis (miR-29b-3p, miR-34a-5p, miR-16-5p, miR-17-5p, miR-107, miR-19b-3p, let-7b-5p, miR-26b-5p, and 127-3p) were able to distinguish between FTD patients and healthy controls. For this study, we enrolled 127 subjects, including 54 patients with FTD, 20 patients with Alzheimer's disease (AD), and 53 healthy controls. The qRT-PCR analysis showed a downregulation of miR-127-3p in FTD compared to controls, while the levels of other miRNAs remained unchanged. Then, miR-127-3p expression was also analyzed in AD patients, finding a different expression between two patient groups. A receiver operating characteristic curve was then created for miR-127-3p to discriminate FTD versus AD (AUC: 0.8986), and versus healthy controls (AUC: 0.8057). In conclusion, miR-127-3p could help to diagnose FTD and to distinguish it from AD.",
keywords = "Biomarker, differential diagnosis, Frontotemporal dementia, miR-127-3p, miRNA",
author = "Paola Piscopo and Margherita Grasso and Maria Puopolo and Emanuela D'Acunto and Giuseppina Talarico and Alessio Crestini and Marina Gasparini and Rosa Campopiano and Stefano Gambardella and Castellano, {Anna Elisa} and Giuseppe Bruno and Denti, {Michela A} and Annamaria Confaloni",
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doi = "10.3233/JAD-180364",
language = "English",
volume = "65",
pages = "455--464",
journal = "Journal of Alzheimer's Disease",
issn = "1387-2877",
publisher = "IOS Press",
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TY - JOUR

T1 - Circulating miR-127-3p as a Potential Biomarker for Differential Diagnosis in Frontotemporal Dementia

AU - Piscopo, Paola

AU - Grasso, Margherita

AU - Puopolo, Maria

AU - D'Acunto, Emanuela

AU - Talarico, Giuseppina

AU - Crestini, Alessio

AU - Gasparini, Marina

AU - Campopiano, Rosa

AU - Gambardella, Stefano

AU - Castellano, Anna Elisa

AU - Bruno, Giuseppe

AU - Denti, Michela A

AU - Confaloni, Annamaria

PY - 2018

Y1 - 2018

N2 - Given the heterogeneous nature of frontotemporal dementia (FTD), sensitive biomarkers are greatly needed for the accurate diagnosis of this neurodegenerative disorder. Circulating miRNAs have been reported as promising biomarkers for neurodegenerative disorders and processes affecting the central nervous system, especially in aging. The objective of the study was to evaluate if some circulating miRNAs linked with apoptosis (miR-29b-3p, miR-34a-5p, miR-16-5p, miR-17-5p, miR-107, miR-19b-3p, let-7b-5p, miR-26b-5p, and 127-3p) were able to distinguish between FTD patients and healthy controls. For this study, we enrolled 127 subjects, including 54 patients with FTD, 20 patients with Alzheimer's disease (AD), and 53 healthy controls. The qRT-PCR analysis showed a downregulation of miR-127-3p in FTD compared to controls, while the levels of other miRNAs remained unchanged. Then, miR-127-3p expression was also analyzed in AD patients, finding a different expression between two patient groups. A receiver operating characteristic curve was then created for miR-127-3p to discriminate FTD versus AD (AUC: 0.8986), and versus healthy controls (AUC: 0.8057). In conclusion, miR-127-3p could help to diagnose FTD and to distinguish it from AD.

AB - Given the heterogeneous nature of frontotemporal dementia (FTD), sensitive biomarkers are greatly needed for the accurate diagnosis of this neurodegenerative disorder. Circulating miRNAs have been reported as promising biomarkers for neurodegenerative disorders and processes affecting the central nervous system, especially in aging. The objective of the study was to evaluate if some circulating miRNAs linked with apoptosis (miR-29b-3p, miR-34a-5p, miR-16-5p, miR-17-5p, miR-107, miR-19b-3p, let-7b-5p, miR-26b-5p, and 127-3p) were able to distinguish between FTD patients and healthy controls. For this study, we enrolled 127 subjects, including 54 patients with FTD, 20 patients with Alzheimer's disease (AD), and 53 healthy controls. The qRT-PCR analysis showed a downregulation of miR-127-3p in FTD compared to controls, while the levels of other miRNAs remained unchanged. Then, miR-127-3p expression was also analyzed in AD patients, finding a different expression between two patient groups. A receiver operating characteristic curve was then created for miR-127-3p to discriminate FTD versus AD (AUC: 0.8986), and versus healthy controls (AUC: 0.8057). In conclusion, miR-127-3p could help to diagnose FTD and to distinguish it from AD.

KW - Biomarker

KW - differential diagnosis

KW - Frontotemporal dementia

KW - miR-127-3p

KW - miRNA

U2 - 10.3233/JAD-180364

DO - 10.3233/JAD-180364

M3 - Article

VL - 65

SP - 455

EP - 464

JO - Journal of Alzheimer's Disease

JF - Journal of Alzheimer's Disease

SN - 1387-2877

IS - 2

ER -