TY - JOUR
T1 - Circulating miR-21, miR-146a and Fas ligand respond to postmenopausal estrogen-based hormone replacement therapy - A study with monozygotic twin pairs
AU - Kangas, Reeta
AU - Pöllänen, Eija
AU - Rippo, Maria Rita
AU - Lanzarini, Catia
AU - Prattichizzo, Francesco
AU - Niskala, Paula
AU - Jylhävä, Juulia
AU - Sipilä, Sarianna
AU - Kaprio, Jaakko
AU - Procopio, Antonio Domenico
AU - Capri, Miriam
AU - Franceschi, Claudio
AU - Olivieri, Fabiola
AU - Kovanen, Vuokko
PY - 2014/12/5
Y1 - 2014/12/5
N2 - Biological aging is associated with physiological deteriorations, which are partly due to changes in the hormonal profile. MicroRNAs regulate various processes associated with cell senescence; differentiation, replication and apoptosis. Serum microRNAs have potential to serve as noninvasive markers for diagnostics/prognostics and therapeutic targets.We analysed the association of estrogen-based hormone replacement therapy (HRT) with selected microRNAs and inflammation markers from the serum, leukocytes and muscle biopsy samples from 54 to 62 year-old postmenopausal monozygotic twins (n=. 11 pairs) discordant for HRT usage. Premenopausal 30-35 year-old women (n=. 8) were used as young controls. We focused on the hormonal aging and on the interaction between HRT use and the modulation of miR-21, miR-146a and classical inflammation markers. Fas-ligand was analysed since it functions in both apoptosis and inflammation.The inflammatory profile was healthier among the premenopausal women compared to the postmenopausal twins. Serum miR-21 and miR-146a levels and FasL concentrations were lower in HRT users compared to their non-using co-twins, demonstrating their responsiveness to HRT. Based on the pairwise FasL analysis, FasL concentration is likely to be genetically controlled. Overall, we suggest that postmenopausal estrogen deficiency sustains the development of "inflamm-aging". Estrogen sensitive, specific circulating microRNAs could be potential, early biomarkers for age-associated physiological deteriorations.
AB - Biological aging is associated with physiological deteriorations, which are partly due to changes in the hormonal profile. MicroRNAs regulate various processes associated with cell senescence; differentiation, replication and apoptosis. Serum microRNAs have potential to serve as noninvasive markers for diagnostics/prognostics and therapeutic targets.We analysed the association of estrogen-based hormone replacement therapy (HRT) with selected microRNAs and inflammation markers from the serum, leukocytes and muscle biopsy samples from 54 to 62 year-old postmenopausal monozygotic twins (n=. 11 pairs) discordant for HRT usage. Premenopausal 30-35 year-old women (n=. 8) were used as young controls. We focused on the hormonal aging and on the interaction between HRT use and the modulation of miR-21, miR-146a and classical inflammation markers. Fas-ligand was analysed since it functions in both apoptosis and inflammation.The inflammatory profile was healthier among the premenopausal women compared to the postmenopausal twins. Serum miR-21 and miR-146a levels and FasL concentrations were lower in HRT users compared to their non-using co-twins, demonstrating their responsiveness to HRT. Based on the pairwise FasL analysis, FasL concentration is likely to be genetically controlled. Overall, we suggest that postmenopausal estrogen deficiency sustains the development of "inflamm-aging". Estrogen sensitive, specific circulating microRNAs could be potential, early biomarkers for age-associated physiological deteriorations.
KW - "Inflamm-aging"
KW - Apoptosis
KW - Estrogen
KW - Hormone replacement therapy
KW - Micrornas
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U2 - 10.1016/j.mad.2014.11.001
DO - 10.1016/j.mad.2014.11.001
M3 - Article
C2 - 25448133
AN - SCOPUS:84919388514
VL - 143-144
SP - 1
EP - 8
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
SN - 0047-6374
ER -