TY - JOUR
T1 - Circulating miRNAs in small extracellular vesicles secreted by a human melanoma xenograft in mouse brains
AU - Guglielmi, Loredana
AU - Nardella, Marta
AU - Musa, Carla
AU - Cifola, Ingrid
AU - Porru, Manuela
AU - Cardinali, Beatrice
AU - Iannetti, Ilaria
AU - Di Pietro, Chiara
AU - Bolasco, Giulia
AU - Palmieri, Valentina
AU - Vilardo, Laura
AU - Panini, Nicolò
AU - Bonaventura, Fabrizio
AU - Papi, Massimiliano
AU - Scavizzi, Ferdinando
AU - Raspa, Marcello
AU - Leonetti, Carlo
AU - Falcone, Germana
AU - Felsani, Armando
AU - D’agnano, Igea
N1 - Funding Information:
Funding: This work was supported by the Italian Ministry for Education, University and Research under the framework of the Flagship Project Interomics to Igea D’Agnano.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/6
Y1 - 2020/6
N2 - The identification of liquid biomarkers remains a major challenge to improve the diagnosis of melanoma patients with brain metastases. Circulating miRNAs packaged into tumor-secreted small extracellular vesicles (sEVs) contribute to tumor progression. To investigate the release of tumor-secreted miRNAs by brain metastasis, we developed a xenograft model where human metastatic melanoma cells were injected intracranially in nude mice. The comprehensive profiles of both free miRNAs and those packaged in sEVs secreted by the melanoma cells in the plasma demonstrated that most (80%) of the sEV-associated miRNAs were also present in serum EVs from a cohort of metastatic melanomas, included in a publicly available dataset. Remarkably, among them, we found three miRNAs (miR-224-5p, miR-130a-3p and miR-21-5p) in sEVs showing a trend of upregulation during melanoma progression. Our model is proven to be valuable for identifying miRNAs in EVs that are unequivocally secreted by melanoma cells in the brain and could be associated to disease progression.
AB - The identification of liquid biomarkers remains a major challenge to improve the diagnosis of melanoma patients with brain metastases. Circulating miRNAs packaged into tumor-secreted small extracellular vesicles (sEVs) contribute to tumor progression. To investigate the release of tumor-secreted miRNAs by brain metastasis, we developed a xenograft model where human metastatic melanoma cells were injected intracranially in nude mice. The comprehensive profiles of both free miRNAs and those packaged in sEVs secreted by the melanoma cells in the plasma demonstrated that most (80%) of the sEV-associated miRNAs were also present in serum EVs from a cohort of metastatic melanomas, included in a publicly available dataset. Remarkably, among them, we found three miRNAs (miR-224-5p, miR-130a-3p and miR-21-5p) in sEVs showing a trend of upregulation during melanoma progression. Our model is proven to be valuable for identifying miRNAs in EVs that are unequivocally secreted by melanoma cells in the brain and could be associated to disease progression.
KW - Circulating miRNAs
KW - Melanoma
KW - Small extracellular vesicles
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U2 - 10.3390/cancers12061635
DO - 10.3390/cancers12061635
M3 - Article
AN - SCOPUS:85086650522
VL - 12
SP - 1
EP - 22
JO - Cancers
JF - Cancers
SN - 2072-6694
IS - 6
M1 - 1635
ER -