Circulating plant miRNAs can regulate human gene expression in vitro

Chiara Pastrello, Mike Tsay, Rosanne McQuaid, Mark Abovsky, Elisa Pasini, Elize Shirdel, Marc Angeli, Tomas Tokar, Joseph Jamnik, Max Kotlyar, Andrea Jurisicova, Joanne Kotsopoulos, Ahmed El-Sohemy, Igor Jurisica

Research output: Contribution to journalArticle

Abstract

While Brassica oleracea vegetables have been linked to cancer prevention, the exact mechanism remains unknown. Regulation of gene expression by cross-species microRNAs has been previously reported; however, its link to cancer suppression remains unexplored. In this study we address both issues. We confirm plant microRNAs in human blood in a large nutrigenomics study cohort and in a randomized dose-controlled trial, finding a significant positive correlation between the daily amount of broccoli consumed and the amount of microRNA in the blood. We also demonstrate that Brassica microRNAs regulate expression of human genes and proteins in vitro, and that microRNAs cooperate with other Brassica-specific compounds in a possible cancer-preventive mechanism. Combined, we provide strong evidence and a possible multimodal mechanism for broccoli in cancer prevention.

Original languageEnglish
Article number32773
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - Sep 8 2016

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Brassica
MicroRNAs
Gene Expression
Neoplasms
Nutrigenomics
Gene Expression Regulation
Vegetables
Cohort Studies
Randomized Controlled Trials
In Vitro Techniques
Proteins

ASJC Scopus subject areas

  • General

Cite this

Pastrello, C., Tsay, M., McQuaid, R., Abovsky, M., Pasini, E., Shirdel, E., ... Jurisica, I. (2016). Circulating plant miRNAs can regulate human gene expression in vitro. Scientific Reports, 6, [32773]. https://doi.org/10.1038/srep32773

Circulating plant miRNAs can regulate human gene expression in vitro. / Pastrello, Chiara; Tsay, Mike; McQuaid, Rosanne; Abovsky, Mark; Pasini, Elisa; Shirdel, Elize; Angeli, Marc; Tokar, Tomas; Jamnik, Joseph; Kotlyar, Max; Jurisicova, Andrea; Kotsopoulos, Joanne; El-Sohemy, Ahmed; Jurisica, Igor.

In: Scientific Reports, Vol. 6, 32773, 08.09.2016.

Research output: Contribution to journalArticle

Pastrello, C, Tsay, M, McQuaid, R, Abovsky, M, Pasini, E, Shirdel, E, Angeli, M, Tokar, T, Jamnik, J, Kotlyar, M, Jurisicova, A, Kotsopoulos, J, El-Sohemy, A & Jurisica, I 2016, 'Circulating plant miRNAs can regulate human gene expression in vitro', Scientific Reports, vol. 6, 32773. https://doi.org/10.1038/srep32773
Pastrello C, Tsay M, McQuaid R, Abovsky M, Pasini E, Shirdel E et al. Circulating plant miRNAs can regulate human gene expression in vitro. Scientific Reports. 2016 Sep 8;6. 32773. https://doi.org/10.1038/srep32773
Pastrello, Chiara ; Tsay, Mike ; McQuaid, Rosanne ; Abovsky, Mark ; Pasini, Elisa ; Shirdel, Elize ; Angeli, Marc ; Tokar, Tomas ; Jamnik, Joseph ; Kotlyar, Max ; Jurisicova, Andrea ; Kotsopoulos, Joanne ; El-Sohemy, Ahmed ; Jurisica, Igor. / Circulating plant miRNAs can regulate human gene expression in vitro. In: Scientific Reports. 2016 ; Vol. 6.
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