Cis-acting elements in the promoter region of the human aldolase C gene

Pasqualina Buono, Lisa de Conciliis, Eleonora Olivetta, Paola Izzo, Francesco Salvatore

Research output: Contribution to journalArticle

Abstract

We investigated the cis-acting sequences involved in the expression of the human aldolase C gene by transient transfections into human neuroblastoma cells (SKNBE). We demonstrate that 420 bp of the 5'-flanking DNA direct at high efficiency the transcription of the CAT reporter gene. A deletion between - 420 bp and - 164 bp causes a 60% decrease of CAT activity. Gel shift and DNase I footprinting analyses revealed four protected elements: A, B, C and D. Competition analyses indicate that Spl or factors sharing a similar sequence specificity bind to elements A and B, but not to elements C and D. Sequence analysis shows a half palindromic ERE motif (GGTCA), in elements B and D. Region D binds a transactivating factor which appears also essential to stabilize the initiation complex.

Original languageEnglish
Pages (from-to)243-249
Number of pages7
JournalFEBS Letters
Volume328
Issue number3
DOIs
Publication statusPublished - Aug 16 1993

Keywords

  • Aldolase C
  • cis-acting elements
  • Gene expression
  • Neuroblastoma cell (SKNBE)
  • Sp1 transacting factor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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    Buono, P., de Conciliis, L., Olivetta, E., Izzo, P., & Salvatore, F. (1993). Cis-acting elements in the promoter region of the human aldolase C gene. FEBS Letters, 328(3), 243-249. https://doi.org/10.1016/0014-5793(93)80936-O