Abstract
Two chromosome-encoded beta-lactamases have been purified from Citrobacter diversus ULA-27. They exhibited slightly different isoelectric points (6.8 and 6.2) and very similar Mr values (congruent to 29,000). Their specificity spectrum was rather wide, since they hydrolysed some cephalosporins with kcat: values similar to those observed with the best penicillin substrates. Cloxacillin, methicillin and imipenem were hydrolysed very slowly. Hydrolysis of azthreonam could not be detected.
| Original language | English |
|---|---|
| Pages (from-to) | 885-890 |
| Number of pages | 6 |
| Journal | Biochemical Journal |
| Volume | 254 |
| Issue number | 3 |
| Publication status | Published - Sep 15 1988 |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Amicosante, G., Oratore, A., Franceschini, N., Maccarrone, M., Strom, R., Galleni, M., & Frère, J. M. (1988). Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties. Biochemical Journal, 254(3), 885-890.