Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties.

G. Amicosante, A. Oratore, N. Franceschini, M. Maccarrone, R. Strom, M. Galleni, J. M. Frère

Research output: Contribution to journalArticlepeer-review


Two chromosome-encoded beta-lactamases have been purified from Citrobacter diversus ULA-27. They exhibited slightly different isoelectric points (6.8 and 6.2) and very similar Mr values (congruent to 29,000). Their specificity spectrum was rather wide, since they hydrolysed some cephalosporins with kcat: values similar to those observed with the best penicillin substrates. Cloxacillin, methicillin and imipenem were hydrolysed very slowly. Hydrolysis of azthreonam could not be detected.

Original languageEnglish
Pages (from-to)885-890
Number of pages6
JournalBiochemical Journal
Issue number3
Publication statusPublished - Sep 15 1988

ASJC Scopus subject areas

  • Biochemistry


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