Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties.

G. Amicosante; A. Oratore; N. Franceschini; M. Maccarrone; R. Strom; M. Galleni; J. M. Frère

Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties.

G. Amicosante, A. Oratore, N. Franceschini, M. Maccarrone, R. Strom, M. Galleni, J. M. Frère

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

Two chromosome-encoded beta-lactamases have been purified from Citrobacter diversus ULA-27. They exhibited slightly different isoelectric points (6.8 and 6.2) and very similar Mr values (congruent to 29,000). Their specificity spectrum was rather wide, since they hydrolysed some cephalosporins with kcat: values similar to those observed with the best penicillin substrates. Cloxacillin, methicillin and imipenem were hydrolysed very slowly. Hydrolysis of azthreonam could not be detected.

Original language	English
Pages (from-to)	885-890
Number of pages	6
Journal	Biochemical Journal
Volume	254
Issue number	3
Publication status	Published - Sep 15 1988

ASJC Scopus subject areas

Biochemistry

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abstract = "Two chromosome-encoded beta-lactamases have been purified from Citrobacter diversus ULA-27. They exhibited slightly different isoelectric points (6.8 and 6.2) and very similar Mr values (congruent to 29,000). Their specificity spectrum was rather wide, since they hydrolysed some cephalosporins with kcat: values similar to those observed with the best penicillin substrates. Cloxacillin, methicillin and imipenem were hydrolysed very slowly. Hydrolysis of azthreonam could not be detected.",

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AU - Amicosante, G.

AU - Oratore, A.

AU - Franceschini, N.

AU - Maccarrone, M.

AU - Strom, R.

AU - Galleni, M.

AU - Frère, J. M.

PY - 1988/9/15

Y1 - 1988/9/15

N2 - Two chromosome-encoded beta-lactamases have been purified from Citrobacter diversus ULA-27. They exhibited slightly different isoelectric points (6.8 and 6.2) and very similar Mr values (congruent to 29,000). Their specificity spectrum was rather wide, since they hydrolysed some cephalosporins with kcat: values similar to those observed with the best penicillin substrates. Cloxacillin, methicillin and imipenem were hydrolysed very slowly. Hydrolysis of azthreonam could not be detected.

AB - Two chromosome-encoded beta-lactamases have been purified from Citrobacter diversus ULA-27. They exhibited slightly different isoelectric points (6.8 and 6.2) and very similar Mr values (congruent to 29,000). Their specificity spectrum was rather wide, since they hydrolysed some cephalosporins with kcat: values similar to those observed with the best penicillin substrates. Cloxacillin, methicillin and imipenem were hydrolysed very slowly. Hydrolysis of azthreonam could not be detected.

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JO - Biochemical Journal

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Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties.

Abstract

ASJC Scopus subject areas

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