Citrulline blood levels as indicators of residual intestinal absorption in patients with short bowel syndrome

Lidia Santarpia, Francesca Catanzano, Margherita Ruoppolo, Lucia Alfonsi, Dino Franco Vitale, Rita Pecce, Fabrizio Pasanisi, Franco Contaldo, Francesco Salvatore

Research output: Contribution to journalArticle

Abstract

Plasma citrulline is known to be a marker of absorptive enterocyte mass in humans. We evaluated whether citrulline and other blood amino acids are indicators of residual small intestinal length and therefore potential predictors of dependence on parenteral nutrition in the long term. We studied 25 patients with short bowel syndrome (SBS) after at least 18 months since last digestive circuit modification; 24 of them were again evaluated 1 year later. Ten patients were weaned off parenteral nutrition and 15 were dependent on parenteral nutrition. Fifty-four healthy volunteers (28 women and 26 men) served as controls. Amino acid levels were determined on serum with high-performance liquid chromatography (HPLC) as well as on blood and serum with tandem mass spectrometry analysis. Five amino acids (citrulline, leucine, isoleucine, valine and tyrosine) were significantly lower in all SBS patients than in controls, whereas glutamine, measured only by HPLC, was significantly higher. Nevertheless, only serum citrulline measured with HPLC was significantly related to small bowel length. We conclude that HPLC remains the reference methodology to evaluate blood or serum amino acid levels in adult population with SBS.

Original languageEnglish
Pages (from-to)137-142
Number of pages6
JournalAnnals of Nutrition and Metabolism
Volume53
Issue number2
DOIs
Publication statusPublished - Nov 2008

Fingerprint

Short Bowel Syndrome
Citrulline
Intestinal Absorption
Parenteral Nutrition
High Pressure Liquid Chromatography
Amino Acids
Serum
Enterocytes
Isoleucine
Valine
Tandem Mass Spectrometry
Glutamine
Leucine
Tyrosine
Healthy Volunteers
Population

Keywords

  • Amino acids
  • Citrulline
  • High-performance liquid chromatography
  • Short bowel syndrome
  • Tandem mass spectrometry

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Santarpia, L., Catanzano, F., Ruoppolo, M., Alfonsi, L., Vitale, D. F., Pecce, R., ... Salvatore, F. (2008). Citrulline blood levels as indicators of residual intestinal absorption in patients with short bowel syndrome. Annals of Nutrition and Metabolism, 53(2), 137-142. https://doi.org/10.1159/000170888

Citrulline blood levels as indicators of residual intestinal absorption in patients with short bowel syndrome. / Santarpia, Lidia; Catanzano, Francesca; Ruoppolo, Margherita; Alfonsi, Lucia; Vitale, Dino Franco; Pecce, Rita; Pasanisi, Fabrizio; Contaldo, Franco; Salvatore, Francesco.

In: Annals of Nutrition and Metabolism, Vol. 53, No. 2, 11.2008, p. 137-142.

Research output: Contribution to journalArticle

Santarpia, L, Catanzano, F, Ruoppolo, M, Alfonsi, L, Vitale, DF, Pecce, R, Pasanisi, F, Contaldo, F & Salvatore, F 2008, 'Citrulline blood levels as indicators of residual intestinal absorption in patients with short bowel syndrome', Annals of Nutrition and Metabolism, vol. 53, no. 2, pp. 137-142. https://doi.org/10.1159/000170888
Santarpia, Lidia ; Catanzano, Francesca ; Ruoppolo, Margherita ; Alfonsi, Lucia ; Vitale, Dino Franco ; Pecce, Rita ; Pasanisi, Fabrizio ; Contaldo, Franco ; Salvatore, Francesco. / Citrulline blood levels as indicators of residual intestinal absorption in patients with short bowel syndrome. In: Annals of Nutrition and Metabolism. 2008 ; Vol. 53, No. 2. pp. 137-142.
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