CL316,243 and cold stress induce heterogeneous expression of UCP1 mRNA and protein in rodent brown adipocytes

Saverio Cinti, Raffaella Cancello, Maria Cristina Zingaretti, Enzo Ceresi, Rita De Matteis, Antonio Giordano, Jean Himms-Hagen, Daniel Ricquier

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Uncoupling protein 1 (UCP1), the mammalian thermogenic mitochondrial protein, is found only in brown adipocytes, but its expression by immunohistochemistry is not homogeneous. Here we present evidence that the non-homogeneous pattern of immunostaining for UCP1 (referred to as the "Harlequin phenomenon") is particularly evident after acute and chronic cold (4C) stimulus and after administration of a specific β3-adrenoceptor agonist (CL316,243). Accordingly, mRNA in situ expression confirmed the UCP1 non-homogeneous pattern of gene activation under conditions of adrenergic stimulus. Furthermore, morphometric analysis of immunogold-stained thin sections showed that UCP1-gold particle density was different among neighboring brown adipocytes with mitochondria of the same size and density. When the adrenergic stimulus was reduced in warm-acclimated animals (28C), UCP1 protein and mRNA expression was reduced and consequently the Harlequin phenomenon was barely visible. These data suggest the existence of an alternative and controlled functional recruitment of brown adipocytes in acute adrenergically stressed animals, possibly to avoid heat and metabolic damage in thermogenically active cells. Of note, the heat shock protein heme oxygenase 1 (HO1) is heterogeneously expressed in adrenergically stimulated brown adipose tissue and, specifically, cells expressing strong immunoreactivity for UCP1 also strongly express HO1.

Original languageEnglish
Pages (from-to)21-31
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Volume50
Issue number1
Publication statusPublished - 2002

Fingerprint

Brown Adipocytes
Rodentia
Messenger RNA
Proteins
Heme Oxygenase-1
Adrenergic Agents
Mitochondrial Size
Brown Adipose Tissue
Mitochondrial Proteins
Heat-Shock Proteins
Gold
Adrenergic Receptors
Transcriptional Activation
disodium (R,R)-5-(2-((2-(3-chlorophenyl)-2-hydroxyethyl)-amino)propyl)-1,3-benzodioxole-2,3-dicarboxylate
Uncoupling Protein 1
Hot Temperature
Immunohistochemistry

Keywords

  • β-adrenoceptor agonist
  • Brown adipose tissue
  • Heat shock protein
  • Heme oxygenase
  • Thermogenesis
  • Uncoupling protein 1

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Cinti, S., Cancello, R., Zingaretti, M. C., Ceresi, E., De Matteis, R., Giordano, A., ... Ricquier, D. (2002). CL316,243 and cold stress induce heterogeneous expression of UCP1 mRNA and protein in rodent brown adipocytes. Journal of Histochemistry and Cytochemistry, 50(1), 21-31.

CL316,243 and cold stress induce heterogeneous expression of UCP1 mRNA and protein in rodent brown adipocytes. / Cinti, Saverio; Cancello, Raffaella; Zingaretti, Maria Cristina; Ceresi, Enzo; De Matteis, Rita; Giordano, Antonio; Himms-Hagen, Jean; Ricquier, Daniel.

In: Journal of Histochemistry and Cytochemistry, Vol. 50, No. 1, 2002, p. 21-31.

Research output: Contribution to journalArticle

Cinti, S, Cancello, R, Zingaretti, MC, Ceresi, E, De Matteis, R, Giordano, A, Himms-Hagen, J & Ricquier, D 2002, 'CL316,243 and cold stress induce heterogeneous expression of UCP1 mRNA and protein in rodent brown adipocytes', Journal of Histochemistry and Cytochemistry, vol. 50, no. 1, pp. 21-31.
Cinti, Saverio ; Cancello, Raffaella ; Zingaretti, Maria Cristina ; Ceresi, Enzo ; De Matteis, Rita ; Giordano, Antonio ; Himms-Hagen, Jean ; Ricquier, Daniel. / CL316,243 and cold stress induce heterogeneous expression of UCP1 mRNA and protein in rodent brown adipocytes. In: Journal of Histochemistry and Cytochemistry. 2002 ; Vol. 50, No. 1. pp. 21-31.
@article{3c784c84bfbd461e9f5a8b072a9646e0,
title = "CL316,243 and cold stress induce heterogeneous expression of UCP1 mRNA and protein in rodent brown adipocytes",
abstract = "Uncoupling protein 1 (UCP1), the mammalian thermogenic mitochondrial protein, is found only in brown adipocytes, but its expression by immunohistochemistry is not homogeneous. Here we present evidence that the non-homogeneous pattern of immunostaining for UCP1 (referred to as the {"}Harlequin phenomenon{"}) is particularly evident after acute and chronic cold (4C) stimulus and after administration of a specific β3-adrenoceptor agonist (CL316,243). Accordingly, mRNA in situ expression confirmed the UCP1 non-homogeneous pattern of gene activation under conditions of adrenergic stimulus. Furthermore, morphometric analysis of immunogold-stained thin sections showed that UCP1-gold particle density was different among neighboring brown adipocytes with mitochondria of the same size and density. When the adrenergic stimulus was reduced in warm-acclimated animals (28C), UCP1 protein and mRNA expression was reduced and consequently the Harlequin phenomenon was barely visible. These data suggest the existence of an alternative and controlled functional recruitment of brown adipocytes in acute adrenergically stressed animals, possibly to avoid heat and metabolic damage in thermogenically active cells. Of note, the heat shock protein heme oxygenase 1 (HO1) is heterogeneously expressed in adrenergically stimulated brown adipose tissue and, specifically, cells expressing strong immunoreactivity for UCP1 also strongly express HO1.",
keywords = "β-adrenoceptor agonist, Brown adipose tissue, Heat shock protein, Heme oxygenase, Thermogenesis, Uncoupling protein 1",
author = "Saverio Cinti and Raffaella Cancello and Zingaretti, {Maria Cristina} and Enzo Ceresi and {De Matteis}, Rita and Antonio Giordano and Jean Himms-Hagen and Daniel Ricquier",
year = "2002",
language = "English",
volume = "50",
pages = "21--31",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "1",

}

TY - JOUR

T1 - CL316,243 and cold stress induce heterogeneous expression of UCP1 mRNA and protein in rodent brown adipocytes

AU - Cinti, Saverio

AU - Cancello, Raffaella

AU - Zingaretti, Maria Cristina

AU - Ceresi, Enzo

AU - De Matteis, Rita

AU - Giordano, Antonio

AU - Himms-Hagen, Jean

AU - Ricquier, Daniel

PY - 2002

Y1 - 2002

N2 - Uncoupling protein 1 (UCP1), the mammalian thermogenic mitochondrial protein, is found only in brown adipocytes, but its expression by immunohistochemistry is not homogeneous. Here we present evidence that the non-homogeneous pattern of immunostaining for UCP1 (referred to as the "Harlequin phenomenon") is particularly evident after acute and chronic cold (4C) stimulus and after administration of a specific β3-adrenoceptor agonist (CL316,243). Accordingly, mRNA in situ expression confirmed the UCP1 non-homogeneous pattern of gene activation under conditions of adrenergic stimulus. Furthermore, morphometric analysis of immunogold-stained thin sections showed that UCP1-gold particle density was different among neighboring brown adipocytes with mitochondria of the same size and density. When the adrenergic stimulus was reduced in warm-acclimated animals (28C), UCP1 protein and mRNA expression was reduced and consequently the Harlequin phenomenon was barely visible. These data suggest the existence of an alternative and controlled functional recruitment of brown adipocytes in acute adrenergically stressed animals, possibly to avoid heat and metabolic damage in thermogenically active cells. Of note, the heat shock protein heme oxygenase 1 (HO1) is heterogeneously expressed in adrenergically stimulated brown adipose tissue and, specifically, cells expressing strong immunoreactivity for UCP1 also strongly express HO1.

AB - Uncoupling protein 1 (UCP1), the mammalian thermogenic mitochondrial protein, is found only in brown adipocytes, but its expression by immunohistochemistry is not homogeneous. Here we present evidence that the non-homogeneous pattern of immunostaining for UCP1 (referred to as the "Harlequin phenomenon") is particularly evident after acute and chronic cold (4C) stimulus and after administration of a specific β3-adrenoceptor agonist (CL316,243). Accordingly, mRNA in situ expression confirmed the UCP1 non-homogeneous pattern of gene activation under conditions of adrenergic stimulus. Furthermore, morphometric analysis of immunogold-stained thin sections showed that UCP1-gold particle density was different among neighboring brown adipocytes with mitochondria of the same size and density. When the adrenergic stimulus was reduced in warm-acclimated animals (28C), UCP1 protein and mRNA expression was reduced and consequently the Harlequin phenomenon was barely visible. These data suggest the existence of an alternative and controlled functional recruitment of brown adipocytes in acute adrenergically stressed animals, possibly to avoid heat and metabolic damage in thermogenically active cells. Of note, the heat shock protein heme oxygenase 1 (HO1) is heterogeneously expressed in adrenergically stimulated brown adipose tissue and, specifically, cells expressing strong immunoreactivity for UCP1 also strongly express HO1.

KW - β-adrenoceptor agonist

KW - Brown adipose tissue

KW - Heat shock protein

KW - Heme oxygenase

KW - Thermogenesis

KW - Uncoupling protein 1

UR - http://www.scopus.com/inward/record.url?scp=0036141928&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036141928&partnerID=8YFLogxK

M3 - Article

C2 - 11748291

AN - SCOPUS:0036141928

VL - 50

SP - 21

EP - 31

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 1

ER -