TY - JOUR
T1 - Clast4, the murine homologue of human eIF4E-Transporter, is highly expressed in developing oocytes and post-translationally modified at meiotic maturation
AU - Villaescusa, J. Carlos
AU - Allard, Patrick
AU - Carminati, Emanuela
AU - Kontogiannea, Maria
AU - Talarico, Daniela
AU - Blasi, Francesco
AU - Farookhi, Riaz
AU - Verrotti, Arturo C.
PY - 2006/2/15
Y1 - 2006/2/15
N2 - In metazoans, translational regulation of a set of maternal mRNAs directs oocyte maturation and early embryogenesis. These transcripts are often kept dormant until their products are spatially and temporally required in development. The interaction between general translation factors (i.e. eIF4E) and their specific interactors influences translation initiation. A search of the protein database for a mouse homologue of the Drosophila Cup protein, a translational repressor during female germ-line development, identified the product of the Clast4 gene. In this report, we show that Clast4 mRNA and protein are highly expressed within the cytoplasm of growing oocytes. The Clast4 protein is stable during this developmental window and post-translationally modified by phosphorylation upon oocyte meiotic maturation. Additionally, we show that Clast4 and eIF4E directly interact by means of a canonical and functional eIF4E-binding motif. Our results suggest that Clast4, similar to Drosophila Cup, may act at the translational level during murine female germ-line development.
AB - In metazoans, translational regulation of a set of maternal mRNAs directs oocyte maturation and early embryogenesis. These transcripts are often kept dormant until their products are spatially and temporally required in development. The interaction between general translation factors (i.e. eIF4E) and their specific interactors influences translation initiation. A search of the protein database for a mouse homologue of the Drosophila Cup protein, a translational repressor during female germ-line development, identified the product of the Clast4 gene. In this report, we show that Clast4 mRNA and protein are highly expressed within the cytoplasm of growing oocytes. The Clast4 protein is stable during this developmental window and post-translationally modified by phosphorylation upon oocyte meiotic maturation. Additionally, we show that Clast4 and eIF4E directly interact by means of a canonical and functional eIF4E-binding motif. Our results suggest that Clast4, similar to Drosophila Cup, may act at the translational level during murine female germ-line development.
KW - Cup
KW - eIF4E
KW - Follicular development
KW - Ovary
KW - Translation initiation
UR - http://www.scopus.com/inward/record.url?scp=33344470641&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33344470641&partnerID=8YFLogxK
U2 - 10.1016/j.gene.2005.09.026
DO - 10.1016/j.gene.2005.09.026
M3 - Article
C2 - 16343815
AN - SCOPUS:33344470641
VL - 367
SP - 101
EP - 109
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1-2
ER -