Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension

Matteo Biancospino, Gwen R. Buel, Carlos A. Niño, Elena Maspero, Rossella Scotto di Perrotolo, Andrea Raimondi, Lisa Redlingshöfer, Janine Weber, Frances M. Brodsky, Kylie J. Walters, Simona Polo

Research output: Contribution to journalArticle

Abstract

Clathrin light chains (CLCa and CLCb) are major constituents of clathrin-coated vesicles. Unique functions for these evolutionary conserved paralogs remain elusive, and their role in clathrin-mediated endocytosis in mammalian cells is debated. Here, we find and structurally characterize a direct and selective interaction between CLCa and the long isoform of the actin motor protein myosin VI, which is expressed exclusively in highly polarized tissues. Using genetically-reconstituted Caco-2 cysts as proxy for polarized epithelia, we provide evidence for coordinated action of myosin VI and CLCa at the apical surface where these proteins are essential for fission of clathrin-coated pits. We further find that myosin VI and Huntingtin-interacting protein 1-related protein (Hip1R) are mutually exclusive interactors with CLCa, and suggest a model for the sequential function of myosin VI and Hip1R in actin-mediated clathrin-coated vesicle budding.

Original languageEnglish
Article number4974
JournalNature Communications
Volume10
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension'. Together they form a unique fingerprint.

  • Cite this

    Biancospino, M., Buel, G. R., Niño, C. A., Maspero, E., di Perrotolo, R. S., Raimondi, A., Redlingshöfer, L., Weber, J., Brodsky, F. M., Walters, K. J., & Polo, S. (2019). Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension. Nature Communications, 10(1), [4974]. https://doi.org/10.1038/s41467-019-12855-6