Claudin-14 Gene Polymorphisms and Urine Calcium Excretion

T Arcidiacono, M Simonini, C Lanzani, L Citterio, E Salvi, C Barlassina, D Spotti, D Cusi, P Manunta, G Vezzoli

Research output: Contribution to journalArticle

Abstract

BACKGROUND AND OBJECTIVES: Claudin-16 and -19 are proteins forming pores for the paracellular reabsorption of divalent cations in the ascending limb of Henle loop; conversely, claudin-14 decreases ion permeability of these pores. Single-nucleotide polymorphisms in gene coding for claudin-14 were associated with kidney stones and calcium excretion. This study aimed to explore the association of claudin-14, claudin-16, and claudin-19 single-nucleotide polymorphisms with calcium excretion.DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: We performed a retrospective observational study of 393 patients with hypertension who were naïve to antihypertensive drugs, in whom we measured 24-hour urine calcium excretion; history of kidney stones was ascertained by interview; 370 of these patients underwent an intravenous 0.9% sodium chloride infusion (2 L in 2 hours) to evaluate the response of calcium excretion in three different 2-hour urine samples collected before, during, and after saline infusion. Genotypes of claudin-14, claudin-16, and claudin-19 were obtained from data of a previous genome-wide association study in the same patients.RESULTS: Thirty-one single-nucleotide polymorphisms of the 3' region of the claudin-14 gene were significantly associated with 24-hour calcium excretion and calcium excretion after saline infusion. The most significant associated single-nucleotide polymorphism was rs219755 (24-hour calcium excretion in GG, 225±124 mg/24 hours; 24-hour calcium excretion in GA, 194±100 mg/24 hours; 24-hour calcium excretion in AA, 124±73 mg/24 hours; P
Original languageEnglish
Pages (from-to)1542-1549
Number of pages8
JournalClinical journal of the American Society of Nephrology : CJASN
Volume13
Issue number10
DOIs
Publication statusPublished - 2018

Fingerprint

Urine
Calcium
Genes
Single Nucleotide Polymorphism
Kidney Calculi
Loop of Henle
claudin 14
Porins
Genome-Wide Association Study
Divalent Cations
Sodium Chloride
Antihypertensive Agents
Observational Studies
Permeability
Retrospective Studies
Genotype
Interviews
Ions
Hypertension
claudin 16

Cite this

Claudin-14 Gene Polymorphisms and Urine Calcium Excretion. / Arcidiacono, T; Simonini, M; Lanzani, C; Citterio, L; Salvi, E; Barlassina, C; Spotti, D; Cusi, D; Manunta, P; Vezzoli, G.

In: Clinical journal of the American Society of Nephrology : CJASN, Vol. 13, No. 10, 2018, p. 1542-1549.

Research output: Contribution to journalArticle

Arcidiacono, T, Simonini, M, Lanzani, C, Citterio, L, Salvi, E, Barlassina, C, Spotti, D, Cusi, D, Manunta, P & Vezzoli, G 2018, 'Claudin-14 Gene Polymorphisms and Urine Calcium Excretion', Clinical journal of the American Society of Nephrology : CJASN, vol. 13, no. 10, pp. 1542-1549. https://doi.org/10.2215/CJN.01770218
Arcidiacono, T ; Simonini, M ; Lanzani, C ; Citterio, L ; Salvi, E ; Barlassina, C ; Spotti, D ; Cusi, D ; Manunta, P ; Vezzoli, G. / Claudin-14 Gene Polymorphisms and Urine Calcium Excretion. In: Clinical journal of the American Society of Nephrology : CJASN. 2018 ; Vol. 13, No. 10. pp. 1542-1549.
@article{d63ad6b1b2e94e62a6fcfd1832e5449e,
title = "Claudin-14 Gene Polymorphisms and Urine Calcium Excretion",
abstract = "BACKGROUND AND OBJECTIVES: Claudin-16 and -19 are proteins forming pores for the paracellular reabsorption of divalent cations in the ascending limb of Henle loop; conversely, claudin-14 decreases ion permeability of these pores. Single-nucleotide polymorphisms in gene coding for claudin-14 were associated with kidney stones and calcium excretion. This study aimed to explore the association of claudin-14, claudin-16, and claudin-19 single-nucleotide polymorphisms with calcium excretion.DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: We performed a retrospective observational study of 393 patients with hypertension who were na{\"i}ve to antihypertensive drugs, in whom we measured 24-hour urine calcium excretion; history of kidney stones was ascertained by interview; 370 of these patients underwent an intravenous 0.9{\%} sodium chloride infusion (2 L in 2 hours) to evaluate the response of calcium excretion in three different 2-hour urine samples collected before, during, and after saline infusion. Genotypes of claudin-14, claudin-16, and claudin-19 were obtained from data of a previous genome-wide association study in the same patients.RESULTS: Thirty-one single-nucleotide polymorphisms of the 3' region of the claudin-14 gene were significantly associated with 24-hour calcium excretion and calcium excretion after saline infusion. The most significant associated single-nucleotide polymorphism was rs219755 (24-hour calcium excretion in GG, 225±124 mg/24 hours; 24-hour calcium excretion in GA, 194±100 mg/24 hours; 24-hour calcium excretion in AA, 124±73 mg/24 hours; P",
author = "T Arcidiacono and M Simonini and C Lanzani and L Citterio and E Salvi and C Barlassina and D Spotti and D Cusi and P Manunta and G Vezzoli",
year = "2018",
doi = "10.2215/CJN.01770218",
language = "English",
volume = "13",
pages = "1542--1549",
journal = "Clinical journal of the American Society of Nephrology : CJASN",
issn = "1555-9041",
publisher = "by the American Society of Nephrology",
number = "10",

}

TY - JOUR

T1 - Claudin-14 Gene Polymorphisms and Urine Calcium Excretion

AU - Arcidiacono, T

AU - Simonini, M

AU - Lanzani, C

AU - Citterio, L

AU - Salvi, E

AU - Barlassina, C

AU - Spotti, D

AU - Cusi, D

AU - Manunta, P

AU - Vezzoli, G

PY - 2018

Y1 - 2018

N2 - BACKGROUND AND OBJECTIVES: Claudin-16 and -19 are proteins forming pores for the paracellular reabsorption of divalent cations in the ascending limb of Henle loop; conversely, claudin-14 decreases ion permeability of these pores. Single-nucleotide polymorphisms in gene coding for claudin-14 were associated with kidney stones and calcium excretion. This study aimed to explore the association of claudin-14, claudin-16, and claudin-19 single-nucleotide polymorphisms with calcium excretion.DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: We performed a retrospective observational study of 393 patients with hypertension who were naïve to antihypertensive drugs, in whom we measured 24-hour urine calcium excretion; history of kidney stones was ascertained by interview; 370 of these patients underwent an intravenous 0.9% sodium chloride infusion (2 L in 2 hours) to evaluate the response of calcium excretion in three different 2-hour urine samples collected before, during, and after saline infusion. Genotypes of claudin-14, claudin-16, and claudin-19 were obtained from data of a previous genome-wide association study in the same patients.RESULTS: Thirty-one single-nucleotide polymorphisms of the 3' region of the claudin-14 gene were significantly associated with 24-hour calcium excretion and calcium excretion after saline infusion. The most significant associated single-nucleotide polymorphism was rs219755 (24-hour calcium excretion in GG, 225±124 mg/24 hours; 24-hour calcium excretion in GA, 194±100 mg/24 hours; 24-hour calcium excretion in AA, 124±73 mg/24 hours; P

AB - BACKGROUND AND OBJECTIVES: Claudin-16 and -19 are proteins forming pores for the paracellular reabsorption of divalent cations in the ascending limb of Henle loop; conversely, claudin-14 decreases ion permeability of these pores. Single-nucleotide polymorphisms in gene coding for claudin-14 were associated with kidney stones and calcium excretion. This study aimed to explore the association of claudin-14, claudin-16, and claudin-19 single-nucleotide polymorphisms with calcium excretion.DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: We performed a retrospective observational study of 393 patients with hypertension who were naïve to antihypertensive drugs, in whom we measured 24-hour urine calcium excretion; history of kidney stones was ascertained by interview; 370 of these patients underwent an intravenous 0.9% sodium chloride infusion (2 L in 2 hours) to evaluate the response of calcium excretion in three different 2-hour urine samples collected before, during, and after saline infusion. Genotypes of claudin-14, claudin-16, and claudin-19 were obtained from data of a previous genome-wide association study in the same patients.RESULTS: Thirty-one single-nucleotide polymorphisms of the 3' region of the claudin-14 gene were significantly associated with 24-hour calcium excretion and calcium excretion after saline infusion. The most significant associated single-nucleotide polymorphism was rs219755 (24-hour calcium excretion in GG, 225±124 mg/24 hours; 24-hour calcium excretion in GA, 194±100 mg/24 hours; 24-hour calcium excretion in AA, 124±73 mg/24 hours; P

U2 - 10.2215/CJN.01770218

DO - 10.2215/CJN.01770218

M3 - Article

VL - 13

SP - 1542

EP - 1549

JO - Clinical journal of the American Society of Nephrology : CJASN

JF - Clinical journal of the American Society of Nephrology : CJASN

SN - 1555-9041

IS - 10

ER -