Cleavage of cystatin C is not associated with multiple sclerosis

Piero Del Boccio, Damiana Pieragostino, Alessandra Lugaresi, Maria Di Ioia, Barbara Pavone, Daniela Travaglini, Simona D'Aguanno, Sergio Bernardini, Paolo Sacchetta, Giorgio Federici, Carmine Di Ilio, Domenico Gambi, Andrea Urbani

Research output: Contribution to journalArticle

Abstract

Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20°C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.

Original languageEnglish
Pages (from-to)201-204
Number of pages4
JournalAnnals of Neurology
Volume62
Issue number2
DOIs
Publication statusPublished - Aug 2007

ASJC Scopus subject areas

  • Neuroscience(all)

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  • Cite this

    Del Boccio, P., Pieragostino, D., Lugaresi, A., Di Ioia, M., Pavone, B., Travaglini, D., D'Aguanno, S., Bernardini, S., Sacchetta, P., Federici, G., Di Ilio, C., Gambi, D., & Urbani, A. (2007). Cleavage of cystatin C is not associated with multiple sclerosis. Annals of Neurology, 62(2), 201-204. https://doi.org/10.1002/ana.20968