Abstract
Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20°C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.
| Original language | English |
|---|---|
| Pages (from-to) | 201-204 |
| Number of pages | 4 |
| Journal | Annals of Neurology |
| Volume | 62 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 2007 |
Fingerprint
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Cleavage of cystatin C is not associated with multiple sclerosis. / Del Boccio, Piero; Pieragostino, Damiana; Lugaresi, Alessandra; Di Ioia, Maria; Pavone, Barbara; Travaglini, Daniela; D'Aguanno, Simona; Bernardini, Sergio; Sacchetta, Paolo; Federici, Giorgio; Di Ilio, Carmine; Gambi, Domenico; Urbani, Andrea.
In: Annals of Neurology, Vol. 62, No. 2, 08.2007, p. 201-204.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cleavage of cystatin C is not associated with multiple sclerosis
AU - Del Boccio, Piero
AU - Pieragostino, Damiana
AU - Lugaresi, Alessandra
AU - Di Ioia, Maria
AU - Pavone, Barbara
AU - Travaglini, Daniela
AU - D'Aguanno, Simona
AU - Bernardini, Sergio
AU - Sacchetta, Paolo
AU - Federici, Giorgio
AU - Di Ilio, Carmine
AU - Gambi, Domenico
AU - Urbani, Andrea
PY - 2007/8
Y1 - 2007/8
N2 - Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20°C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.
AB - Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20°C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.
UR - http://www.scopus.com/inward/record.url?scp=35148898851&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35148898851&partnerID=8YFLogxK
U2 - 10.1002/ana.20968
DO - 10.1002/ana.20968
M3 - Article
C2 - 17006926
AN - SCOPUS:35148898851
VL - 62
SP - 201
EP - 204
JO - Annals of Neurology
JF - Annals of Neurology
SN - 0364-5134
IS - 2
ER -