Abstract
Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20°C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.
Original language | English |
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Pages (from-to) | 201-204 |
Number of pages | 4 |
Journal | Annals of Neurology |
Volume | 62 |
Issue number | 2 |
DOIs | |
Publication status | Published - Aug 2007 |
ASJC Scopus subject areas
- Neuroscience(all)