Cleavage of cystatin C is not associated with multiple sclerosis

Piero Del Boccio, Damiana Pieragostino, Alessandra Lugaresi, Maria Di Ioia, Barbara Pavone, Daniela Travaglini, Simona D'Aguanno, Sergio Bernardini, Paolo Sacchetta, Giorgio Federici, Carmine Di Ilio, Domenico Gambi, Andrea Urbani

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20°C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.

Original languageEnglish
Pages (from-to)201-204
Number of pages4
JournalAnnals of Neurology
Volume62
Issue number2
DOIs
Publication statusPublished - Aug 2007

Fingerprint

Cystatin C
Multiple Sclerosis
Cerebrospinal Fluid
Proteomics
Protein Isoforms

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Del Boccio, P., Pieragostino, D., Lugaresi, A., Di Ioia, M., Pavone, B., Travaglini, D., ... Urbani, A. (2007). Cleavage of cystatin C is not associated with multiple sclerosis. Annals of Neurology, 62(2), 201-204. https://doi.org/10.1002/ana.20968

Cleavage of cystatin C is not associated with multiple sclerosis. / Del Boccio, Piero; Pieragostino, Damiana; Lugaresi, Alessandra; Di Ioia, Maria; Pavone, Barbara; Travaglini, Daniela; D'Aguanno, Simona; Bernardini, Sergio; Sacchetta, Paolo; Federici, Giorgio; Di Ilio, Carmine; Gambi, Domenico; Urbani, Andrea.

In: Annals of Neurology, Vol. 62, No. 2, 08.2007, p. 201-204.

Research output: Contribution to journalArticle

Del Boccio, P, Pieragostino, D, Lugaresi, A, Di Ioia, M, Pavone, B, Travaglini, D, D'Aguanno, S, Bernardini, S, Sacchetta, P, Federici, G, Di Ilio, C, Gambi, D & Urbani, A 2007, 'Cleavage of cystatin C is not associated with multiple sclerosis', Annals of Neurology, vol. 62, no. 2, pp. 201-204. https://doi.org/10.1002/ana.20968
Del Boccio P, Pieragostino D, Lugaresi A, Di Ioia M, Pavone B, Travaglini D et al. Cleavage of cystatin C is not associated with multiple sclerosis. Annals of Neurology. 2007 Aug;62(2):201-204. https://doi.org/10.1002/ana.20968
Del Boccio, Piero ; Pieragostino, Damiana ; Lugaresi, Alessandra ; Di Ioia, Maria ; Pavone, Barbara ; Travaglini, Daniela ; D'Aguanno, Simona ; Bernardini, Sergio ; Sacchetta, Paolo ; Federici, Giorgio ; Di Ilio, Carmine ; Gambi, Domenico ; Urbani, Andrea. / Cleavage of cystatin C is not associated with multiple sclerosis. In: Annals of Neurology. 2007 ; Vol. 62, No. 2. pp. 201-204.
@article{edd1d4e4d49546788792651ced0d4881,
title = "Cleavage of cystatin C is not associated with multiple sclerosis",
abstract = "Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20°C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.",
author = "{Del Boccio}, Piero and Damiana Pieragostino and Alessandra Lugaresi and {Di Ioia}, Maria and Barbara Pavone and Daniela Travaglini and Simona D'Aguanno and Sergio Bernardini and Paolo Sacchetta and Giorgio Federici and {Di Ilio}, Carmine and Domenico Gambi and Andrea Urbani",
year = "2007",
month = "8",
doi = "10.1002/ana.20968",
language = "English",
volume = "62",
pages = "201--204",
journal = "Annals of Neurology",
issn = "0364-5134",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

TY - JOUR

T1 - Cleavage of cystatin C is not associated with multiple sclerosis

AU - Del Boccio, Piero

AU - Pieragostino, Damiana

AU - Lugaresi, Alessandra

AU - Di Ioia, Maria

AU - Pavone, Barbara

AU - Travaglini, Daniela

AU - D'Aguanno, Simona

AU - Bernardini, Sergio

AU - Sacchetta, Paolo

AU - Federici, Giorgio

AU - Di Ilio, Carmine

AU - Gambi, Domenico

AU - Urbani, Andrea

PY - 2007/8

Y1 - 2007/8

N2 - Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20°C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.

AB - Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20°C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.

UR - http://www.scopus.com/inward/record.url?scp=35148898851&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35148898851&partnerID=8YFLogxK

U2 - 10.1002/ana.20968

DO - 10.1002/ana.20968

M3 - Article

C2 - 17006926

AN - SCOPUS:35148898851

VL - 62

SP - 201

EP - 204

JO - Annals of Neurology

JF - Annals of Neurology

SN - 0364-5134

IS - 2

ER -