Cleavage of cystatin C is not associated with multiple sclerosis

Piero Del Boccio; Damiana Pieragostino; Alessandra Lugaresi; Maria Di Ioia; Barbara Pavone; Daniela Travaglini; Simona D'Aguanno; Sergio Bernardini; Paolo Sacchetta; Giorgio Federici; Carmine Di Ilio; Domenico Gambi; Andrea Urbani

doi:10.1002/ana.20968

Cleavage of cystatin C is not associated with multiple sclerosis

Piero Del Boccio, Damiana Pieragostino, Alessandra Lugaresi, Maria Di Ioia, Barbara Pavone, Daniela Travaglini, Simona D'Aguanno, Sergio Bernardini, Paolo Sacchetta, Giorgio Federici, Carmine Di Ilio, Domenico Gambi, Andrea Urbani

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20°C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.

Original language	English
Pages (from-to)	201-204
Number of pages	4
Journal	Annals of Neurology
Volume	62
Issue number	2
DOIs	https://doi.org/10.1002/ana.20968
Publication status	Published - Aug 2007

ASJC Scopus subject areas

Neuroscience(all)

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title = "Cleavage of cystatin C is not associated with multiple sclerosis",

abstract = "Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20°C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.",

author = "{Del Boccio}, Piero and Damiana Pieragostino and Alessandra Lugaresi and {Di Ioia}, Maria and Barbara Pavone and Daniela Travaglini and Simona D'Aguanno and Sergio Bernardini and Paolo Sacchetta and Giorgio Federici and {Di Ilio}, Carmine and Domenico Gambi and Andrea Urbani",

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doi = "10.1002/ana.20968",

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T1 - Cleavage of cystatin C is not associated with multiple sclerosis

AU - Del Boccio, Piero

AU - Pieragostino, Damiana

AU - Lugaresi, Alessandra

AU - Di Ioia, Maria

AU - Pavone, Barbara

AU - Travaglini, Daniela

AU - D'Aguanno, Simona

AU - Bernardini, Sergio

AU - Sacchetta, Paolo

AU - Federici, Giorgio

AU - Di Ilio, Carmine

AU - Gambi, Domenico

AU - Urbani, Andrea

PY - 2007/8

Y1 - 2007/8

N2 - Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20°C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.

AB - Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20°C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.

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Cleavage of cystatin C is not associated with multiple sclerosis

Abstract

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