Clinical and cytological findings in limbal stem cell deficiency

Marta Sacchetti, Alessandro Lambiase, Magdalena Cortes, Roberto Sgrulletta, Sergio Bonini, Daniela Merlo, Stefano Bonini

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Purpose: To evaluate and relate the clinical (including corneal sensitivity and tear function) and cytological (presence of goblet cells and cytokeratin 3- and 19-positive cells) features of limbal stem cell deficiency (LSCD). Methods: Twenty-nine patients (44 eyes) with a clinical diagnosis of LSCD participated in this study. Corneal signs (epithelial alterations, superficial neovascularisation and stromal scarring) and cytological findings (presence of goblet cells and cytokeratins 3 and 19) were evaluated and scored (from 0 to 3) from each of the five corneal sectors. Corneal sensitivity (Cochet-Bonnet aesthesiometer) and tear function (Schirmer test and BUT) were also assessed. Cytological scores were correlated statistically with both corneal signs and sensitivity values. Results: Cytokeratin 19-positive cells were found in 82% of corneal impression cytology samples, while goblet cells were identified in only 59% of these same samples. Cytokeratin 3-positive cells were present in 61% of LSCD eyes and in all unaffected eyes. Corneal sensitivity was significantly decreased in affected eyes compared with contralateral, healthy eyes (1.6±1.7 cm versus 5.7±0.3 cm). Tear function tests did not show significant changes. In LSCD eyes, goblet cells and cytokeratin 19-positive cells on the corneal surface were significantly correlated to corneal epithelial alterations and to corneal superficial neovascularisation (p

Original languageEnglish
Pages (from-to)870-876
Number of pages7
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume243
Issue number9
DOIs
Publication statusPublished - Sep 2005

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Keratin-19
Keratin-3
Goblet Cells
Stem Cells
Tears
Corneal Neovascularization
Cicatrix
Cell Biology

Keywords

  • Corneal sensitivity
  • Cytokeratin 19
  • Cytokeratin 3
  • Impression cytology
  • Limbal stem cell deficiency

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Clinical and cytological findings in limbal stem cell deficiency. / Sacchetti, Marta; Lambiase, Alessandro; Cortes, Magdalena; Sgrulletta, Roberto; Bonini, Sergio; Merlo, Daniela; Bonini, Stefano.

In: Graefe's Archive for Clinical and Experimental Ophthalmology, Vol. 243, No. 9, 09.2005, p. 870-876.

Research output: Contribution to journalArticle

Sacchetti, M, Lambiase, A, Cortes, M, Sgrulletta, R, Bonini, S, Merlo, D & Bonini, S 2005, 'Clinical and cytological findings in limbal stem cell deficiency', Graefe's Archive for Clinical and Experimental Ophthalmology, vol. 243, no. 9, pp. 870-876. https://doi.org/10.1007/s00417-005-1159-0
Sacchetti, Marta ; Lambiase, Alessandro ; Cortes, Magdalena ; Sgrulletta, Roberto ; Bonini, Sergio ; Merlo, Daniela ; Bonini, Stefano. / Clinical and cytological findings in limbal stem cell deficiency. In: Graefe's Archive for Clinical and Experimental Ophthalmology. 2005 ; Vol. 243, No. 9. pp. 870-876.
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