Clinical evaluation of TRCReady M.TB for rapid automated detection of M. tuberculosis complex in respiratory samples

Research output: Contribution to journalArticle

Abstract

S E T T ING: Timely diagnosis of tuberculosis (TB) is essential for effectively controlling and managing the disease. Although international guidelines recommend acid-fast bacilli staining and culture as the 'gold standard', new molecular methods are available to safely and rapidly identify positive samples. OBJECTIVE: To evaluate the performance of the newer and fully automated version of a molecular assay for rRNA amplification (TRCReadyw M.TB) on 1028 respiratory samples collected from 378 patients for its possible use as a reliable screening method. Results were evaluated using culture as the reference test. RESULTS : Of four diagnostic protocols employed, best results were obtained when TRCReady M.TB was used together with microscopy on the first respiratory sample, followed by microscopy alone on a second one. The sensitivity and specificity were respectively 97% and 100%, with a turnaround time of 24 h. We propose a possible laboratory algorithm for rapid identification of patients with TB. CONCLUSIONS : TRCReady offers the advantages of full automation and avoidance of cross-contamination. As such, it should be considered as a more economical option for TB screening than other commercial assays that are currently available.

Original languageEnglish
Pages (from-to)260-264
Number of pages5
JournalInternational Journal of Tuberculosis and Lung Disease
Volume23
Issue number2
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Tuberculosis
Microscopy
Automation
Bacillus
Guidelines
Staining and Labeling
Sensitivity and Specificity
Acids

Keywords

  • Molecular assay
  • Rapid diagnosis
  • Tuberculosis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

Cite this

@article{6109fe4f12b44d8598f4bec78c5d2a26,
title = "Clinical evaluation of TRCReady M.TB for rapid automated detection of M. tuberculosis complex in respiratory samples",
abstract = "S E T T ING: Timely diagnosis of tuberculosis (TB) is essential for effectively controlling and managing the disease. Although international guidelines recommend acid-fast bacilli staining and culture as the 'gold standard', new molecular methods are available to safely and rapidly identify positive samples. OBJECTIVE: To evaluate the performance of the newer and fully automated version of a molecular assay for rRNA amplification (TRCReadyw M.TB) on 1028 respiratory samples collected from 378 patients for its possible use as a reliable screening method. Results were evaluated using culture as the reference test. RESULTS : Of four diagnostic protocols employed, best results were obtained when TRCReady M.TB was used together with microscopy on the first respiratory sample, followed by microscopy alone on a second one. The sensitivity and specificity were respectively 97{\%} and 100{\%}, with a turnaround time of 24 h. We propose a possible laboratory algorithm for rapid identification of patients with TB. CONCLUSIONS : TRCReady offers the advantages of full automation and avoidance of cross-contamination. As such, it should be considered as a more economical option for TB screening than other commercial assays that are currently available.",
keywords = "Molecular assay, Rapid diagnosis, Tuberculosis",
author = "A. Mazzarelli and A. Cannas and C. Venditti and S. D'Arezzo and {De Giuli}, C. and S. Truffa and O. Butera and F. Petroni and M. Paglia and A. Vulcano and F. Palmieri and C. Nisii and {Di Caro}, A.",
year = "2019",
month = "1",
day = "1",
doi = "10.5588/ijtld.18.0355",
language = "English",
volume = "23",
pages = "260--264",
journal = "International Journal of Tuberculosis and Lung Disease",
issn = "1027-3719",
publisher = "International Union against Tubercul. and Lung Dis.",
number = "2",

}

TY - JOUR

T1 - Clinical evaluation of TRCReady M.TB for rapid automated detection of M. tuberculosis complex in respiratory samples

AU - Mazzarelli, A.

AU - Cannas, A.

AU - Venditti, C.

AU - D'Arezzo, S.

AU - De Giuli, C.

AU - Truffa, S.

AU - Butera, O.

AU - Petroni, F.

AU - Paglia, M.

AU - Vulcano, A.

AU - Palmieri, F.

AU - Nisii, C.

AU - Di Caro, A.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - S E T T ING: Timely diagnosis of tuberculosis (TB) is essential for effectively controlling and managing the disease. Although international guidelines recommend acid-fast bacilli staining and culture as the 'gold standard', new molecular methods are available to safely and rapidly identify positive samples. OBJECTIVE: To evaluate the performance of the newer and fully automated version of a molecular assay for rRNA amplification (TRCReadyw M.TB) on 1028 respiratory samples collected from 378 patients for its possible use as a reliable screening method. Results were evaluated using culture as the reference test. RESULTS : Of four diagnostic protocols employed, best results were obtained when TRCReady M.TB was used together with microscopy on the first respiratory sample, followed by microscopy alone on a second one. The sensitivity and specificity were respectively 97% and 100%, with a turnaround time of 24 h. We propose a possible laboratory algorithm for rapid identification of patients with TB. CONCLUSIONS : TRCReady offers the advantages of full automation and avoidance of cross-contamination. As such, it should be considered as a more economical option for TB screening than other commercial assays that are currently available.

AB - S E T T ING: Timely diagnosis of tuberculosis (TB) is essential for effectively controlling and managing the disease. Although international guidelines recommend acid-fast bacilli staining and culture as the 'gold standard', new molecular methods are available to safely and rapidly identify positive samples. OBJECTIVE: To evaluate the performance of the newer and fully automated version of a molecular assay for rRNA amplification (TRCReadyw M.TB) on 1028 respiratory samples collected from 378 patients for its possible use as a reliable screening method. Results were evaluated using culture as the reference test. RESULTS : Of four diagnostic protocols employed, best results were obtained when TRCReady M.TB was used together with microscopy on the first respiratory sample, followed by microscopy alone on a second one. The sensitivity and specificity were respectively 97% and 100%, with a turnaround time of 24 h. We propose a possible laboratory algorithm for rapid identification of patients with TB. CONCLUSIONS : TRCReady offers the advantages of full automation and avoidance of cross-contamination. As such, it should be considered as a more economical option for TB screening than other commercial assays that are currently available.

KW - Molecular assay

KW - Rapid diagnosis

KW - Tuberculosis

UR - http://www.scopus.com/inward/record.url?scp=85065404482&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85065404482&partnerID=8YFLogxK

U2 - 10.5588/ijtld.18.0355

DO - 10.5588/ijtld.18.0355

M3 - Article

C2 - 30808461

AN - SCOPUS:85065404482

VL - 23

SP - 260

EP - 264

JO - International Journal of Tuberculosis and Lung Disease

JF - International Journal of Tuberculosis and Lung Disease

SN - 1027-3719

IS - 2

ER -