Clinical expression of big-big prolactin and influence of macroprolactinemia upon immunodiagnostic tests

Simona Galoiu, G. Kertesz, C. Somma, M. Coculescu, T. Brue

Research output: Contribution to journalArticle

Abstract

In some humans, the big and big-big prolactin variants represent the majority of circulating prolactin, considered to be without biological activity. Aims: to establish the clinical expression of macroprolactinemia and the interference with immunodiagnostic tests in a randomized group of 84 consecutive patients with hyperprolactinemia. IRMA and electrochemiluminescence (Elecsys) were used for PRL assay; gel filtration chromatography (GFC) and protein A precipitation test were used to reveal macroprolactinemia. Results: Macroprolactinemia was found in 16 out of 84 patients (group A), 62 patients had hyperprolactinemia of other causes (group B) and 6 had normal PRL levels and normal GFC (group C). Of 16 patients with macroprolactinemia, 6 showed normal PRL with IRMA and hyperprolactinemia with Elecsys. The difference between the two methods used (Δ = PRL determined by Elecsys, -PRL determined by IRMA) correlated with big big PRL level determined by GFC with Elecsys in all patients. The strongest correlation was found in patients with macroprolactinemia (group A, r=0.82, p

Original languageEnglish
Pages (from-to)31-42
Number of pages12
JournalActa Endocrinologica
Volume1
Issue number1
Publication statusPublished - 2005

Keywords

  • Big-big prolactin
  • Hyperprolactinemia
  • Immunoassay
  • Macroprolactinemia

ASJC Scopus subject areas

  • Endocrinology

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