Clinical findings with implications for genetic testing in families with clustering of colorectal cancer

Juul T. Wijnen, Hans F A Vasen, P. Meera Khan, Aeilko H. Zwinderman, Heleen Van Der Klift, Adri Mulder, Carli Tops, Pål Møller, Riccardo Fodde, Fred Menko, Babs Taal, Fokko Nagengast, Han Brunner, Jan Kleibeuker, Rolf Sijmons, Gerrit Griffioen, Annette Bröcker-Vriends, Egbert Bakker, Inge Van Leeuwen-Cornelisse, Anne Meijers-HeijboerDick Lindhout, Martijn Breuning, Jan Post, Cees Schaap, Jaran Apold, Ketil Heimdal, Lucio Bertario, Marie Luise Bisgaard, Petr Goetz

Research output: Contribution to journalArticle

Abstract

Background Germ-line mutations in DNA mismatch-repair genes (MSH2, MLH1, PMS1, PMS2, and MSH6) cause susceptibility to hereditary nonpolyposis colorectal cancer. We assessed the prevalence of MSH2 and MLH1 mutations in families suspected of having hereditary nonpolyposis colorectal cancer and evaluated whether clinical findings can predict the outcome of genetic testing. Methods We used denaturing gradient gel electrophoresis to identify MSH2 and MLH1 mutations in 184 kindreds with familial clustering of colorectal cancer or other cancers associated with hereditary nonpolyposis colorectal cancer. Information on the site of cancer, the age at diagnosis, and the number of affected family members was obtained from all families. Results Mutations of MSH2 or MLH1 were found in 47 of the 184 kindreds (26 percent). Clinical factors associated with these mutations were early age at diagnosis of colorectal cancer, the occurrence in the kindred of endometrial cancer or tumors of the small intestine, a higher number of family members with colorectal or endometrial cancer, the presence of multiple colorectal cancers or both colorectal and endometrial cancers in a single family member, and fulfillment of the Amsterdam criteria for the diagnosis of hereditary nonpolyposis colorectal cancer (at least three family members in two or more successive generations must have colorectal cancer, one of whom is a first- degree relative of the other two; cancer must be diagnosed before the age of 50 in at least one family member; and familial adenomatous polyposis must be ruled out). Multivariate analysis showed that a younger age at diagnosis of colorectal cancer, fulfillment of the Amsterdam criteria, and the presence of endometrial cancer in the kindred were independent predictors of germ-line mutations of MSH2 or MLH1. These results were used to devise a logistic model for estimating the likelihood of a mutation in MSH2 and MLH1. Conclusions Assessment of clinical findings can improve the rate of detection of mutations of DNA mismatch-repair genes in families suspected of having hereditary nonpolyposis colorectal cancer.

Original languageEnglish
Pages (from-to)511-518
Number of pages8
JournalNew England Journal of Medicine
Volume339
Issue number8
DOIs
Publication statusPublished - Aug 20 1998

ASJC Scopus subject areas

  • Medicine(all)

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    Wijnen, J. T., Vasen, H. F. A., Khan, P. M., Zwinderman, A. H., Van Der Klift, H., Mulder, A., Tops, C., Møller, P., Fodde, R., Menko, F., Taal, B., Nagengast, F., Brunner, H., Kleibeuker, J., Sijmons, R., Griffioen, G., Bröcker-Vriends, A., Bakker, E., Van Leeuwen-Cornelisse, I., ... Goetz, P. (1998). Clinical findings with implications for genetic testing in families with clustering of colorectal cancer. New England Journal of Medicine, 339(8), 511-518. https://doi.org/10.1056/NEJM199808203390804