Clinical-grade quality platelet-rich plasma releasate (PRP-R/SRGF) from CaCl2-activated platelet concentrates promoted expansion of mesenchymal stromal cells

C. Borghese, F. Agostini, C. Durante, A. Colombatti, M. Mazzucato, D. Aldinucci

Research output: Contribution to journalArticlepeer-review

Abstract

Background and Objectives: The aim of our study was to test a platelet-rich plasma releasate (PRP-R/SRGF) from CaCl2-activated platelets as a source of growth factors for the expansion of mesenchymal stromal cells (MSCs). PRP-R/SRGF, obtained with a low-cost procedure, is characterized by a reduced variability of growth factor release. Materials and Methods: PRP-R/SRGF is a clinical-grade quality solution obtained from CaCl2-activated platelets. Its activity was evaluated by measuring the proliferation, the phenotype, the differentiation potential and the immunosuppressive properties of MSCs derived from bone marrow (BM) and adipose tissue (AT). Results: PRP-R/SRGF was more active than FBS to expand BM- and AT-derived MSCs. PRP-R/SRGF treatment did not affect the expression of typical MSCs surface markers, neither MSCs differentiation potential nor their capability to inhibit activated T-cell proliferation. Conclusions: The clinical-grade PRP-R/SRGF may be used in the clinical setting for the expansion of MSCs.

Original languageEnglish
Pages (from-to)197-205
Number of pages9
JournalVox Sanguinis
Volume111
Issue number2
DOIs
Publication statusPublished - Aug 1 2016

Keywords

  • apheresis
  • cellular therapy
  • cytokine
  • mesenchymal stem cells
  • platelet concentrates
  • regenerative medicine

ASJC Scopus subject areas

  • Hematology

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