Clinical impact of a new automated system employed for peripheral blood stem cell collection

Claudia Del Fante, Cesare Perotti, Gianluca Viarengo, Laura Bellotti, Cristina Parisi, Andrea Marchesi, Carmine Tinelli, Laura Salvaneschi

Research output: Contribution to journalArticle

Abstract

At the moment, PBSC collections can be performed using semi-automated or automated cell separator devices. The automated methods offer the advantages of a decreased working load for dedicated personnel and high standardization of the collection procedure. Herein we report our single institutional experience in 80 PBSC collections employing the new automated COM.TEC Fresenius autoMNC program that provides the ability to predict the total number of CD34+ cells collected, based on the pre-leukapheresis CD34+ cell count in peripheral blood. Fourty-eight patients and 21 healthy donors were mobilized with chemotherapy + G-CSF or G-CSF alone, respectively. Eighty leukapheresis collections were performed starting with a CD34+ cell count in peripheral blood at least of 20/μL. Collection parameters and related side effects were evaluated. The mean CD34+ cell collection efficiency in patients and donors was 81.8% (sd 27.6) and 95.1% (sd 15.6), respectively. The mean difference between real and predicted CD34+ cells was +30.2% (sd 92.9) for patients and +4.6% (sd 30.3) for donors. The mean leukapheresis bag volume was 240.7 ml (sd 67.3) and 310.3 ml (sd 86.8) with a mean HCT of 10.9% (sd 34.4) and 9.2% (sd 3.9) for patients and donors, respectively. The automated PBSC collection with the new program COM.TEC Fresenius autoMNC demonstrated a very high CD34+ cell collection efficiency. Moreover, the ability to predict the CD34+ cell yield permits improved management of the leukapheresis collection, with the only disadvantage of larger leukapheresis volume and higher hematocrit.

Original languageEnglish
Pages (from-to)227-232
Number of pages6
JournalJournal of Clinical Apheresis
Volume21
Issue number4
DOIs
Publication statusPublished - Dec 2006

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Keywords

  • Automated collection
  • Leukapheresis
  • Stem cell transplant

ASJC Scopus subject areas

  • Hematology

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