Clinical severity and molecular typing of human rhinovirus C strains during a fall outbreak affecting hospitalized patients

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Background: The circulation rate and the clinical severity of infections caused by members of the new human rhinovirus C (HRV-C) species remain to be defined. Objectives: To investigate the epidemiologic and clinical impact of HRV-C strains in a fall outbreak interesting hospitalized patients. Study design: HRV species (A-C) were determined by phylogenetic analysis following amplification of two genome regions (5′NCR and VP4/VP2) by RT-PCR. HRV species were correlated with age, respiratory tract involvement, clinical symptoms, and HRV load in respiratory secretions. Results: During the first week of the period October-November 2008, single HRV infections were associated with 95% of all respiratory syndromes affecting hospitalized patients. Then, HRV infections (single + coinfections) interested about 90% of positive samples until the end of October, when they declined in frequency until reaching about 30% at the end of November. Overall, 104 HRV strains were detected and, of these, 90 could be classified by phylogenetic analysis, as follows: 45 HRV-A, 12 HRV-B, 28 HRV-C, and 5 human enterovirus D strains. HRV-C identity was confirmed by detection of cis-acting replication elements (cre) in 23/23 strains. As for severity of respiratory syndromes, unlike HRV-A and HRV-B strains, HRV-C strains were responsible for a significantly higher rate (p <0.05) of lower respiratory tract infections in the pediatric as compared to adult patient population. Conclusions: HRV-C strains have been shown to circulate at a rate intermediate between HRV-A and HRV-B strains, showing a greater degree of clinical severity in the pediatric population.

Original languageEnglish
Pages (from-to)311-317
Number of pages7
JournalJournal of Clinical Virology
Volume45
Issue number4
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Molecular Typing
Rhinovirus
Disease Outbreaks
Human Enterovirus D
Infection
Pediatrics
Coinfection
Respiratory Tract Infections
Respiratory System
Population
Genome
Polymerase Chain Reaction

Keywords

  • Human rhinovirus C
  • Real-time RT-PCR
  • Respiratory tract infections
  • Rhinovirus load
  • Rhinovirus type

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

@article{240aa8e3d9d8470395c333a36cee5d3f,
title = "Clinical severity and molecular typing of human rhinovirus C strains during a fall outbreak affecting hospitalized patients",
abstract = "Background: The circulation rate and the clinical severity of infections caused by members of the new human rhinovirus C (HRV-C) species remain to be defined. Objectives: To investigate the epidemiologic and clinical impact of HRV-C strains in a fall outbreak interesting hospitalized patients. Study design: HRV species (A-C) were determined by phylogenetic analysis following amplification of two genome regions (5′NCR and VP4/VP2) by RT-PCR. HRV species were correlated with age, respiratory tract involvement, clinical symptoms, and HRV load in respiratory secretions. Results: During the first week of the period October-November 2008, single HRV infections were associated with 95{\%} of all respiratory syndromes affecting hospitalized patients. Then, HRV infections (single + coinfections) interested about 90{\%} of positive samples until the end of October, when they declined in frequency until reaching about 30{\%} at the end of November. Overall, 104 HRV strains were detected and, of these, 90 could be classified by phylogenetic analysis, as follows: 45 HRV-A, 12 HRV-B, 28 HRV-C, and 5 human enterovirus D strains. HRV-C identity was confirmed by detection of cis-acting replication elements (cre) in 23/23 strains. As for severity of respiratory syndromes, unlike HRV-A and HRV-B strains, HRV-C strains were responsible for a significantly higher rate (p <0.05) of lower respiratory tract infections in the pediatric as compared to adult patient population. Conclusions: HRV-C strains have been shown to circulate at a rate intermediate between HRV-A and HRV-B strains, showing a greater degree of clinical severity in the pediatric population.",
keywords = "Human rhinovirus C, Real-time RT-PCR, Respiratory tract infections, Rhinovirus load, Rhinovirus type",
author = "A. Piralla and F. Rovida and G. Campanini and V. Rognoni and A. Marchi and F. Locatelli and G. Gerna",
year = "2009",
month = "8",
doi = "10.1016/j.jcv.2009.04.016",
language = "English",
volume = "45",
pages = "311--317",
journal = "Journal of Clinical Virology",
issn = "1386-6532",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Clinical severity and molecular typing of human rhinovirus C strains during a fall outbreak affecting hospitalized patients

AU - Piralla, A.

AU - Rovida, F.

AU - Campanini, G.

AU - Rognoni, V.

AU - Marchi, A.

AU - Locatelli, F.

AU - Gerna, G.

PY - 2009/8

Y1 - 2009/8

N2 - Background: The circulation rate and the clinical severity of infections caused by members of the new human rhinovirus C (HRV-C) species remain to be defined. Objectives: To investigate the epidemiologic and clinical impact of HRV-C strains in a fall outbreak interesting hospitalized patients. Study design: HRV species (A-C) were determined by phylogenetic analysis following amplification of two genome regions (5′NCR and VP4/VP2) by RT-PCR. HRV species were correlated with age, respiratory tract involvement, clinical symptoms, and HRV load in respiratory secretions. Results: During the first week of the period October-November 2008, single HRV infections were associated with 95% of all respiratory syndromes affecting hospitalized patients. Then, HRV infections (single + coinfections) interested about 90% of positive samples until the end of October, when they declined in frequency until reaching about 30% at the end of November. Overall, 104 HRV strains were detected and, of these, 90 could be classified by phylogenetic analysis, as follows: 45 HRV-A, 12 HRV-B, 28 HRV-C, and 5 human enterovirus D strains. HRV-C identity was confirmed by detection of cis-acting replication elements (cre) in 23/23 strains. As for severity of respiratory syndromes, unlike HRV-A and HRV-B strains, HRV-C strains were responsible for a significantly higher rate (p <0.05) of lower respiratory tract infections in the pediatric as compared to adult patient population. Conclusions: HRV-C strains have been shown to circulate at a rate intermediate between HRV-A and HRV-B strains, showing a greater degree of clinical severity in the pediatric population.

AB - Background: The circulation rate and the clinical severity of infections caused by members of the new human rhinovirus C (HRV-C) species remain to be defined. Objectives: To investigate the epidemiologic and clinical impact of HRV-C strains in a fall outbreak interesting hospitalized patients. Study design: HRV species (A-C) were determined by phylogenetic analysis following amplification of two genome regions (5′NCR and VP4/VP2) by RT-PCR. HRV species were correlated with age, respiratory tract involvement, clinical symptoms, and HRV load in respiratory secretions. Results: During the first week of the period October-November 2008, single HRV infections were associated with 95% of all respiratory syndromes affecting hospitalized patients. Then, HRV infections (single + coinfections) interested about 90% of positive samples until the end of October, when they declined in frequency until reaching about 30% at the end of November. Overall, 104 HRV strains were detected and, of these, 90 could be classified by phylogenetic analysis, as follows: 45 HRV-A, 12 HRV-B, 28 HRV-C, and 5 human enterovirus D strains. HRV-C identity was confirmed by detection of cis-acting replication elements (cre) in 23/23 strains. As for severity of respiratory syndromes, unlike HRV-A and HRV-B strains, HRV-C strains were responsible for a significantly higher rate (p <0.05) of lower respiratory tract infections in the pediatric as compared to adult patient population. Conclusions: HRV-C strains have been shown to circulate at a rate intermediate between HRV-A and HRV-B strains, showing a greater degree of clinical severity in the pediatric population.

KW - Human rhinovirus C

KW - Real-time RT-PCR

KW - Respiratory tract infections

KW - Rhinovirus load

KW - Rhinovirus type

UR - http://www.scopus.com/inward/record.url?scp=67649647908&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67649647908&partnerID=8YFLogxK

U2 - 10.1016/j.jcv.2009.04.016

DO - 10.1016/j.jcv.2009.04.016

M3 - Article

VL - 45

SP - 311

EP - 317

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

SN - 1386-6532

IS - 4

ER -