Abstract
Background and Objectives. p53 status and CD38 antigen are biological factors influencing response to therapy and clinical course in B-cell chronic lymphocytic leukemia (B-CLL). This study tests the hypothesis that soluble p53 alone and in association with CD38 can enucleate B-CLL subsets at worse prognosis. Design and Methods. Wild and mutant forms of p53 protein were evaluated in 197 B-CLL patients at diagnosis or before progression by an immunoenzymatic method in plasma using an anti-p53 monoclonal antibody. CD38 expression was analyzed by a multicolor flow cytometric assay. Results. Higher levels of both soluble p53 (sp53) and CD38 were significantly correlated with intermediate and high Rai stages, with higher β2-microglobulin and soluble CD23 values, determined at diagnosis. Shorter overall survival (OS) and progression-free survival (PFS) were both observed in sp53+ and CD38+ patients (p 60 years) were independent prognostic factors whereas sp53 showed only a tendency towards statistical significance.
| Original language | English |
|---|---|
| Pages (from-to) | 1468-1475 |
| Number of pages | 8 |
| Journal | Haematologica |
| Volume | 89 |
| Issue number | 12 |
| Publication status | Published - Dec 2004 |
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Keywords
- B-CLL
- CD38
- Prognosis
- Soluble p53
ASJC Scopus subject areas
- Hematology
Cite this
Clinical significance of soluble p53 protein in B-cell chronic lymphocytic leukemia. / Ilaria Del Principe, Maria; Del Poeta, Giovanni; Venditti, Adriano; Buccisano, Francesco; Maurillo, Luca; Marini, Rita; Cox, Maria Christina; Panetta, Paola; Suppo, Giovanna; Degan, Massimo; Bruno, Antonio; Gattei, Valter; Amadori, Sergio.
In: Haematologica, Vol. 89, No. 12, 12.2004, p. 1468-1475.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Clinical significance of soluble p53 protein in B-cell chronic lymphocytic leukemia
AU - Ilaria Del Principe, Maria
AU - Del Poeta, Giovanni
AU - Venditti, Adriano
AU - Buccisano, Francesco
AU - Maurillo, Luca
AU - Marini, Rita
AU - Cox, Maria Christina
AU - Panetta, Paola
AU - Suppo, Giovanna
AU - Degan, Massimo
AU - Bruno, Antonio
AU - Gattei, Valter
AU - Amadori, Sergio
PY - 2004/12
Y1 - 2004/12
N2 - Background and Objectives. p53 status and CD38 antigen are biological factors influencing response to therapy and clinical course in B-cell chronic lymphocytic leukemia (B-CLL). This study tests the hypothesis that soluble p53 alone and in association with CD38 can enucleate B-CLL subsets at worse prognosis. Design and Methods. Wild and mutant forms of p53 protein were evaluated in 197 B-CLL patients at diagnosis or before progression by an immunoenzymatic method in plasma using an anti-p53 monoclonal antibody. CD38 expression was analyzed by a multicolor flow cytometric assay. Results. Higher levels of both soluble p53 (sp53) and CD38 were significantly correlated with intermediate and high Rai stages, with higher β2-microglobulin and soluble CD23 values, determined at diagnosis. Shorter overall survival (OS) and progression-free survival (PFS) were both observed in sp53+ and CD38+ patients (p 60 years) were independent prognostic factors whereas sp53 showed only a tendency towards statistical significance.
AB - Background and Objectives. p53 status and CD38 antigen are biological factors influencing response to therapy and clinical course in B-cell chronic lymphocytic leukemia (B-CLL). This study tests the hypothesis that soluble p53 alone and in association with CD38 can enucleate B-CLL subsets at worse prognosis. Design and Methods. Wild and mutant forms of p53 protein were evaluated in 197 B-CLL patients at diagnosis or before progression by an immunoenzymatic method in plasma using an anti-p53 monoclonal antibody. CD38 expression was analyzed by a multicolor flow cytometric assay. Results. Higher levels of both soluble p53 (sp53) and CD38 were significantly correlated with intermediate and high Rai stages, with higher β2-microglobulin and soluble CD23 values, determined at diagnosis. Shorter overall survival (OS) and progression-free survival (PFS) were both observed in sp53+ and CD38+ patients (p 60 years) were independent prognostic factors whereas sp53 showed only a tendency towards statistical significance.
KW - B-CLL
KW - CD38
KW - Prognosis
KW - Soluble p53
UR - http://www.scopus.com/inward/record.url?scp=10444268007&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=10444268007&partnerID=8YFLogxK
M3 - Article
C2 - 15590397
AN - SCOPUS:10444268007
VL - 89
SP - 1468
EP - 1475
JO - Haematologica
JF - Haematologica
SN - 0390-6078
IS - 12
ER -