Clinical toxicity of cryopreserved circulating progenitor cells infusion

A. Zambelli, G. Poggi, G. A. Da Prada, P. Pedrazzoli, A. Cuomo, D. Miotti, C. Perotti, P. Preti, G. Robustellidella Cuna

Research output: Contribution to journalArticle

Abstract

Background: We evaluated the infusion-related toxicity of cryopreserved autologous circulating progenitor cells transplanted in 22 patients receiving high dose chemotherapy and stem cells transplantation for malignancy. Materials and Methods: Progenitor cells were collected following mobilization with chemotherapy plus filgrastim and stored in liquid nitrogen in the presence of 10% dimethylsulfoxide (DMSO). Before infusion of the graft, patients were medicated with mannitol, hydrocortisone and clorphenamine. The amount of DMSO infused as well as the number of dead and damaged cells were evaluated as possible cause of toxicity. Results: Eleven patients (50%) experienced symptoms related to graft infusion, nausea and vomiting being the most common adverse events. Hypotension was documented in 3 patients (one of them developing transient bradycardia resolved with atropin administration) and one had hypertension with tachycardia. Other observed side effects were: chest tightness (2 pts), fever and chills (3 pts), associated with abdominal cramps (2 pts). 7 out of 8 (88%) patients infused with greater than 30 mL volume of DMSO experienced side-effects, the grade of toxicity being significantly less in those receiving lower amount (<30 mL) of DMSO. Two out of 4 pts who received the highest number of dead cells (> 10 x 10 9) developed toxicity. Conclusions: In our experience the infusion of cryopreserved peripheral blood progenitors caused minor to moderate toxicity in most cases and, when present, side effects were observed only during infusion. The amount of DMSO present in the graft is related to the grade of toxicity.

Original languageEnglish
Pages (from-to)4705-4708
Number of pages4
JournalAnticancer Research
Volume18
Issue number6 B
Publication statusPublished - 1998

Fingerprint

Dimethyl Sulfoxide
Stem Cells
Transplants
Drug Therapy
Chills
Colic
Stem Cell Transplantation
Mannitol
Bradycardia
Tachycardia
Hypotension
Nausea
Vomiting
Hydrocortisone
Fever
Nitrogen
Thorax
Hypertension
Neoplasms

Keywords

  • Autologous PBSC transplantation
  • Graft infusion
  • Toxicity

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Zambelli, A., Poggi, G., Da Prada, G. A., Pedrazzoli, P., Cuomo, A., Miotti, D., ... Cuna, G. R. (1998). Clinical toxicity of cryopreserved circulating progenitor cells infusion. Anticancer Research, 18(6 B), 4705-4708.

Clinical toxicity of cryopreserved circulating progenitor cells infusion. / Zambelli, A.; Poggi, G.; Da Prada, G. A.; Pedrazzoli, P.; Cuomo, A.; Miotti, D.; Perotti, C.; Preti, P.; Cuna, G. Robustellidella.

In: Anticancer Research, Vol. 18, No. 6 B, 1998, p. 4705-4708.

Research output: Contribution to journalArticle

Zambelli, A, Poggi, G, Da Prada, GA, Pedrazzoli, P, Cuomo, A, Miotti, D, Perotti, C, Preti, P & Cuna, GR 1998, 'Clinical toxicity of cryopreserved circulating progenitor cells infusion', Anticancer Research, vol. 18, no. 6 B, pp. 4705-4708.
Zambelli, A. ; Poggi, G. ; Da Prada, G. A. ; Pedrazzoli, P. ; Cuomo, A. ; Miotti, D. ; Perotti, C. ; Preti, P. ; Cuna, G. Robustellidella. / Clinical toxicity of cryopreserved circulating progenitor cells infusion. In: Anticancer Research. 1998 ; Vol. 18, No. 6 B. pp. 4705-4708.
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AU - Zambelli, A.

AU - Poggi, G.

AU - Da Prada, G. A.

AU - Pedrazzoli, P.

AU - Cuomo, A.

AU - Miotti, D.

AU - Perotti, C.

AU - Preti, P.

AU - Cuna, G. Robustellidella

PY - 1998

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N2 - Background: We evaluated the infusion-related toxicity of cryopreserved autologous circulating progenitor cells transplanted in 22 patients receiving high dose chemotherapy and stem cells transplantation for malignancy. Materials and Methods: Progenitor cells were collected following mobilization with chemotherapy plus filgrastim and stored in liquid nitrogen in the presence of 10% dimethylsulfoxide (DMSO). Before infusion of the graft, patients were medicated with mannitol, hydrocortisone and clorphenamine. The amount of DMSO infused as well as the number of dead and damaged cells were evaluated as possible cause of toxicity. Results: Eleven patients (50%) experienced symptoms related to graft infusion, nausea and vomiting being the most common adverse events. Hypotension was documented in 3 patients (one of them developing transient bradycardia resolved with atropin administration) and one had hypertension with tachycardia. Other observed side effects were: chest tightness (2 pts), fever and chills (3 pts), associated with abdominal cramps (2 pts). 7 out of 8 (88%) patients infused with greater than 30 mL volume of DMSO experienced side-effects, the grade of toxicity being significantly less in those receiving lower amount (<30 mL) of DMSO. Two out of 4 pts who received the highest number of dead cells (> 10 x 10 9) developed toxicity. Conclusions: In our experience the infusion of cryopreserved peripheral blood progenitors caused minor to moderate toxicity in most cases and, when present, side effects were observed only during infusion. The amount of DMSO present in the graft is related to the grade of toxicity.

AB - Background: We evaluated the infusion-related toxicity of cryopreserved autologous circulating progenitor cells transplanted in 22 patients receiving high dose chemotherapy and stem cells transplantation for malignancy. Materials and Methods: Progenitor cells were collected following mobilization with chemotherapy plus filgrastim and stored in liquid nitrogen in the presence of 10% dimethylsulfoxide (DMSO). Before infusion of the graft, patients were medicated with mannitol, hydrocortisone and clorphenamine. The amount of DMSO infused as well as the number of dead and damaged cells were evaluated as possible cause of toxicity. Results: Eleven patients (50%) experienced symptoms related to graft infusion, nausea and vomiting being the most common adverse events. Hypotension was documented in 3 patients (one of them developing transient bradycardia resolved with atropin administration) and one had hypertension with tachycardia. Other observed side effects were: chest tightness (2 pts), fever and chills (3 pts), associated with abdominal cramps (2 pts). 7 out of 8 (88%) patients infused with greater than 30 mL volume of DMSO experienced side-effects, the grade of toxicity being significantly less in those receiving lower amount (<30 mL) of DMSO. Two out of 4 pts who received the highest number of dead cells (> 10 x 10 9) developed toxicity. Conclusions: In our experience the infusion of cryopreserved peripheral blood progenitors caused minor to moderate toxicity in most cases and, when present, side effects were observed only during infusion. The amount of DMSO present in the graft is related to the grade of toxicity.

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