Clinical validity of a continuous colorimetric method for serum lipase

G. V. Melzi d'Eril, T. Bosoni, R. Moratti, M. Ventrucci, A. Fumagalli, G. Tarenghi

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Abstract

The clinical validity of a continuous colorimetric method for measuring pancreatic lipase was assessed. 1,2-Diacylglycerol containing long-chain fatty acid residues was used as substrate, and the method was adapted to a discrete analyser. The dynamic range was ascertained up to at least 30-fold the upper reference limit. Precision tests on three control sera yielded overall CVs of 4.6% (mean value 21 U/l), 2.4% (115 U/I), and 1.0% (386 U/l), respectively. Using serum samples from normal subjects and patients with pancreatic and non-pancreatic disorders, the present method was compared with a turbidimetric method (r = 0.997; n = 281) and a homogeneous enzyme immunoassay (r = 0.987; n = 93). The reference interval established on 121 healthy subjects was 8-57 U/l (central 95th percentile, median 22 U/l). The sensitivity of this lipase assay in the diagnosis of acute pancreatitis (100%, median 5.6-fold the upper reference limit) was equal to that of the pancreatic isoamylase assay, and higher than that of the total oc-amylase assay (88.2%); the specificity for acute pancreatis with respect to a group of patients with acute and chronic non-pancreatic abdominal diseases (91%) was higher than that of both pancreatic isoamylase (76%) and total α-amylase (71%).

Original languageEnglish
Pages (from-to)439-444
Number of pages6
JournalEuropean Journal of Clinical Chemistry and Clinical Biochemistry
Volume30
Issue number7
Publication statusPublished - 1992

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ASJC Scopus subject areas

  • Clinical Biochemistry

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Melzi d'Eril, G. V., Bosoni, T., Moratti, R., Ventrucci, M., Fumagalli, A., & Tarenghi, G. (1992). Clinical validity of a continuous colorimetric method for serum lipase. European Journal of Clinical Chemistry and Clinical Biochemistry, 30(7), 439-444.