Clock gene expression in mouse kidney and testis: Analysis of periodical and dynamical patterns

Gianluigi Mazzoccoli, M. Francavilla, F. Giuliani, F. Aucella, M. Vinciguerra, V. Pazienza, A. Piepoli, G. Benegiamo, S. Liu, Y. Cai

Research output: Contribution to journalArticle

Abstract

Molecular clocks drive circadian rhythmicity of cellular functions in peripheral tissues and organs, kidney included, whereas in the testis this clockwork seems constitutively active. We have evaluated the periodicity and the dynamics of expression of the clock genes BMAL1, CLOCK, PER1, PER2, CRY1, CRY2 and REV ERBα over 24 h in the kidney and testis using a mouse model. The periodicity was explored by single cosinor, and dynamics were explored by calculation of fractional variations of gene expression related to time intervals. Kidney and testis were harvested at 4-h intervals over a 24-h period from eight-week-old C57BL/6 male mice housed individually on a 12 h light (L)-dark (D) cycle (lights on at 08:00 h; lights off at 20:00 h) and mRNA was extracted and analyzed by Quantitative Real-time Reverse Transcription PCR. A statistically significant difference was evidenced between kidney and testis for the original values of expression level of BMAL1, PER1, PER2 CRY1, CRY2 and REV ERBα. A statistically significant difference was evidenced between kidney and testis for the fractional variation of BMAL1, PER2, CRY1, CRY2 and REV ERBα. A significant 24-h rhythmic component was found for BMAL1, CLOCK, PER1, PER2, CRY1, CRY2 and REV ERBα in the kidney, whereas no core clock gene showed circadian rhythmicity in the testis. Fractional variations provided significant circadian rhythms for BMAL1, PER2, CRY, CRY2 and REV ERBα in the kidney, whereas in the testis the fractional variation calculations showed no circadian rhythmicity, but quantitative comparison showed statistically significant differences in only 16.7% of the time points studied. In conclusion, in the kidney the clock gene machinery shows circadian oscillation of mRNA levels and time-related variations in the rate of change of clock gene expression. In the testis the clock genes do not show circadian rhythmicity of expression and the dynamics of variation are not characterized by a periodical pattern, but are quantitatively similar to those observed in the kidney. These data suggest that in the testis the clock gene machinery shows a tissue-specific pattern of function and clock genes may play a different role in the testis with regard to other peripheral tissues, maybe in relation to the presence of developmental and differentiation phenomena.

Original languageEnglish
Pages (from-to)303-311
Number of pages9
JournalJournal of Biological Regulators and Homeostatic Agents
Volume26
Issue number2
Publication statusPublished - Apr 2012

Fingerprint

Testis
Kidney
Gene Expression
Periodicity
Genes
Light
Messenger RNA
Circadian Clocks
Photoperiod
Circadian Rhythm
Reverse Transcription
Polymerase Chain Reaction

Keywords

  • Circadian rhythm
  • Clock gene
  • Fractional variation
  • Kidney
  • Testis

ASJC Scopus subject areas

  • Oncology
  • Endocrinology, Diabetes and Metabolism
  • Physiology (medical)
  • Immunology and Allergy
  • Immunology
  • Endocrinology
  • Physiology
  • Cancer Research

Cite this

Clock gene expression in mouse kidney and testis : Analysis of periodical and dynamical patterns. / Mazzoccoli, Gianluigi; Francavilla, M.; Giuliani, F.; Aucella, F.; Vinciguerra, M.; Pazienza, V.; Piepoli, A.; Benegiamo, G.; Liu, S.; Cai, Y.

In: Journal of Biological Regulators and Homeostatic Agents, Vol. 26, No. 2, 04.2012, p. 303-311.

Research output: Contribution to journalArticle

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