Clodronate inhibits angiogenesis in vitro and in vivo

Domenico Ribatti, N. Maruotti, B. Nico, V. Longo, D. Mangieri, A. Vacca, F. P. Cantatore

Research output: Contribution to journalArticlepeer-review


The effects of amino-bisphosphonate clodronate on endothelial cell functions involved in angiogenesis, namely proliferation and morphogenesis on matrigel were tested in vitro, whereas its effects on angiogenesis were studied in vivo. This was performed by using the chick embryo chorioallantoic membrane (CAM) assay. In vitro, clodronate inhibited the endothelial cell proliferation in a dose-dependent fashion, peaking at 30 μM. At the same concentration, clodronate inhibited the fibroblast growth factor-2 (FGF-2)- induced capillary-like tube formation in the morphogenesis assay on matrigel. In vivo, when tested with the CAM assay, clodronate again displayed the capability to inhibit FGF-2- induced angiogenesis. Overall, these results suggest that antiangiogenesis by clodronate can be used to treat a wide spectrum of angiogenesis-dependent diseases, including certain chronic inflammatory diseases and cancer.

Original languageEnglish
Pages (from-to)1109-1112
Number of pages4
JournalOncology Reports
Issue number5
Publication statusPublished - May 2008


  • Antiangiogenesis
  • Bisphosphonate
  • Chorioallantoic membrane
  • Clodronate
  • Endothelial cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


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