Clonal analysis of individual marrow-repopulating cells after experimental peripheral blood progenitor cell transplantion

K. Zsuzsanna Nagy, Stephanie Laufs, Bernhard Gentner, Sonja Naundorf, Klaus Kuehlcke, Julian Topaly, Eike C. Buss, W. Jens Zeller, Stefan Fruehauf

Research output: Contribution to journalArticlepeer-review

Abstract

Methods to analyze the clonality of an adverse event in preclinical or clinical retroviral stem cell gene therapy protocols are needed. We analyzed the progeny of retrovirally transduced human peripheral blood progenitor cells (PBPCs) after transplantation and engraftment in immune-deficient mice. The integration site of the provirus serves as a unique tag of the individual transduced PBPC. A plasmid library of junctions between proviral and human genomic DNA was generated. We were able to detect individual transduced cell clones that amounted to 0.14%-0.0001% of chimeric bone marrow cells. This is the first report in which the contribution of individual marrow-repopulating cells to human hematopoiesis is directly quantified.

Original languageEnglish
Pages (from-to)570-579
Number of pages10
JournalStem Cells
Volume22
Issue number4
Publication statusPublished - 2004

Keywords

  • Experimental PBPC transplantation
  • SCID-repopulating cells
  • Single cell analysis

ASJC Scopus subject areas

  • Cell Biology

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