TY - JOUR
T1 - Clonal analysis of T lymphocyte response to an isolated class I disparity
AU - Reinsmoen, Nancy
AU - Anichini, Andrea
AU - Bach, Fritz H.
PY - 1983
Y1 - 1983
N2 - A bulk primed lymphocyte reagent generated in a class II identical class I (HLA-B) disparate sibling combination demonstrated both cytotoxic {cell mediated lympholysis (CML)} and proliferative {i.e., primed LD (lymphocyte) typing (PLT)} reactivity associated with the class I antigen, Bw62. Cells from this bulk population were plated by limiting dilution and cloned by micromanipulation. Three functional groups of clones were isolated. Some clones derived were found specifically to proliferate to and lyse cells bearing the Bw62 antigen. Based on such reactivities, these clones were analogous to the class of antigen-driven, helper cell independent cytotoxic (HITc) clones previously reported from our laboratories. Other clones responsed proliferatively to stimulation by Bw62 positive cells but were not cytotoxic, thus fitting characteristics of Th, although it will be necessary to test such clones for their ability to produce Interleukin 2 (IL-2). In addition, conventional cytotoxic clones which did not proliferate to, but did lyse cells bearing the Bw62 antigen were isolated. The results were consistent with the existence of both HITc and Tc mediated cytotoxicity generated against this isolated class I disparity.
AB - A bulk primed lymphocyte reagent generated in a class II identical class I (HLA-B) disparate sibling combination demonstrated both cytotoxic {cell mediated lympholysis (CML)} and proliferative {i.e., primed LD (lymphocyte) typing (PLT)} reactivity associated with the class I antigen, Bw62. Cells from this bulk population were plated by limiting dilution and cloned by micromanipulation. Three functional groups of clones were isolated. Some clones derived were found specifically to proliferate to and lyse cells bearing the Bw62 antigen. Based on such reactivities, these clones were analogous to the class of antigen-driven, helper cell independent cytotoxic (HITc) clones previously reported from our laboratories. Other clones responsed proliferatively to stimulation by Bw62 positive cells but were not cytotoxic, thus fitting characteristics of Th, although it will be necessary to test such clones for their ability to produce Interleukin 2 (IL-2). In addition, conventional cytotoxic clones which did not proliferate to, but did lyse cells bearing the Bw62 antigen were isolated. The results were consistent with the existence of both HITc and Tc mediated cytotoxicity generated against this isolated class I disparity.
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U2 - 10.1016/0198-8859(83)90037-X
DO - 10.1016/0198-8859(83)90037-X
M3 - Article
C2 - 6227593
AN - SCOPUS:0021055324
VL - 8
SP - 195
EP - 206
JO - Human Immunology
JF - Human Immunology
SN - 0198-8859
IS - 3
ER -