Clonal CD8+ TCR-Vβ expanded populations with effector memory phenotype in Churg Strauss Syndrome

Giuseppe Guida, Antonella Vallario, Stefania Stella, Monica Boita, Paola Circosta, Sara Mariani, Giuseppina Prato, Enrico Heffler, Roberta Bergia, Antonino Sottile, Giovanni Rolla, Alessandro Cignetti

Research output: Contribution to journalArticlepeer-review

Abstract

Churg Strauss Syndrome (CSS) is a systemic vasculitis in which oligoclonal T cell expansions might be involved in the pathogenesis. Combined analysis of TCR-Vβ expression profile by flow cytometry and of TCR gene rearrangement by heteroduplex PCR was used to detect and characterize T cell expansions in 8 CSS patients, 10 asthmatics and 42 healthy subjects. In all CSS patients one or two Vβ families were expanded among CD8+ cells, with an effector memory phenotype apt to populate tissues and inflammatory sites. Heteroduplex PCR showed the presence of one or more clonal TCR rearrangements, which reveals monoclonal or oligoclonal T cells subpopulations. After purification with a Vβ specific monoclonal antibody, each CD8+/Vβ+ expanded family showed a single TCR rearrangement, clearly suggestive of monoclonality. All CD8+ expansions were detectable throughout the disease course. TCR-Vβ expanded or deleted populations were not observed in asthmatic patients. Clonal CD8+/Vβ+ T cell expansions might be useful as a disease marker.

Original languageEnglish
Pages (from-to)94-102
Number of pages9
JournalClinical Immunology
Volume128
Issue number1
DOIs
Publication statusPublished - Jul 2008

Keywords

  • Churg Strauss Syndrome
  • Flow cytometry
  • Gene rearrangement
  • T cell Receptor
  • T-lymphocyte

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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