Clonal relatedness and conserved integron structures in epidemiologically unrelated Pseudomonas aeruginosa strains producing the VIM-1 metallo-β-lactamase from different Italian hospitals

Maria Letizia Riccio, Lucia Pallecchi, Jean Denis Docquier, Stefania Cresti, Maria Rosaria Catania, Laura Pagani, Cristina Lagatolla, Giuseppe Cornaglia, Roberta Fontana, Gian Maria Rossolini

Research output: Contribution to journalArticlepeer-review

Abstract

Three epidemiologically independent Pseudomonas aeruginosa isolates, representative of the first VIM-1 metallo-β-lactamase producers detected at three different hospitals in northern Italy, were investigated to determine their genomic relatedness and to compare the structures of the genetic supports for the VIM-1 determinants. The three isolates, all of serotype O11, appeared to be clonally related according to the results of genotyping by macrorestriction analysis of genomic DNA by pulsed-field gel electrophoresis and random amplification of polymorphic DNA. Investigation of the genetic support for the blaVIM-1 determinant revealed that it was carried on identical or almost identical integrons (named In70.2 and In70.3) located within a conserved genomic context. The integrons were structurally related to In70 and In110, two plasmid-borne blaVIM-1-containing integrons from Achromobacter xylosoxidans and Pseudomonas putida isolates, respectively, from the same geographic area (northern Italy) and were found to be inserted close to the res site of a Tn5051-like transposon, different from any of those described previously, that was apparently carried on the bacterial chromosome. The present findings suggest that the three VIM-1-producing isolates are members of the same clonal complex which have been spreading in hospitals in northern Italy since the late 1990s and point to a common ancestry of their bla VIM-1-containing integrons.

Original languageEnglish
Pages (from-to)104-110
Number of pages7
JournalAntimicrobial Agents and Chemotherapy
Volume49
Issue number1
DOIs
Publication statusPublished - Jan 2005

ASJC Scopus subject areas

  • Pharmacology (medical)

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