Clonal structure, extended-spectrum β-lactamases,and acquired AmpC-type cephalosporinases of Escherichia coli populations colonizing patients in rehabilitation centers in four countries

R. Izdebski, A. Baraniak, J. Fiett, A. Adler, M. Kazma, J. Salomon, C. Lawrence, A. Rossini, A. Salvia, J. Vidal Samso, J. Fierro, M. Paul, Y. Lerman, S. Malhotra-Kumar, C. Lammens, H. Goossens, W. Hryniewicz, C. Brun-Buisson, Y. Carmeli, M. Gniadkowski

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The prospective project MOSAR was conducted in five rehabilitation units: the Berck Maritime Hôpital (Berck, France), Fondazione Santa Lucia (Rome, Italy), Guttmann Institute (GI; Barcelona, Spain), and Loewenstein Hospital and Tel-Aviv Souraski Medical Center (TA) (Tel-Aviv, Israel). Patients were screened for carriage of Enterobacteriaceae resistant to expanded-spectrum cephalosporins (ESCs) from admission until discharge. The aim of this study was to characterize the clonal structure, extended-spectrum β-lactamases (ESBLs), and acquired AmpC-like cephalosporinases in the Escherichia coli populations collected. A total of 376 isolates were randomly selected. The overall number of sequence types (STs) was 76, including 7 STs that grouped at least 10 isolates from at least three centers each, namely, STs 10, 38, 69, 131, 405, 410, and 648. These clones comprised 65.2% of all isolates, and ST131 alone comprised 41.2%. Of 54 STs observed only in one center, some STs played a locally significant role, like ST156 and ST393 in GI or ST372 and ST398 in TA. Among 16 new STs, five arose from evolution within the ST10 and ST131 clonal complexes. ESBLs and AmpCs accounted for 94.7% and 5.6% of the ESC-hydrolyzing β-lactamases, respectively, being dominated by the CTX-M-like enzymes (79.9%), followed by the SHV (13.5%) and CMY-2 (5.3%) types. CTX-M-15 was the most prevalent β-lactamase overall (40.6%); other ubiquitous enzymes were CTX-M-14 and CMY-2. Almost none of the common clones correlated strictly with one β-lactamase; although 58.7% of ST131 isolates produced CTX-M-15, the clone also expressed nine other enzymes. A number of clone variants with specific pulsed-field gel electrophoresis and ESBL types were spread in some locales, potentially representing newly emerging E. coli epidemic strains.

Original languageEnglish
Pages (from-to)309-316
Number of pages8
JournalAntimicrobial Agents and Chemotherapy
Volume57
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

Rehabilitation Centers
Clone Cells
Escherichia coli
Cephalosporins
Population
Enzymes
Pulsed Field Gel Electrophoresis
Israel
Enterobacteriaceae
Spain
Italy
France
Rehabilitation
galantide

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

Clonal structure, extended-spectrum β-lactamases,and acquired AmpC-type cephalosporinases of Escherichia coli populations colonizing patients in rehabilitation centers in four countries. / Izdebski, R.; Baraniak, A.; Fiett, J.; Adler, A.; Kazma, M.; Salomon, J.; Lawrence, C.; Rossini, A.; Salvia, A.; Vidal Samso, J.; Fierro, J.; Paul, M.; Lerman, Y.; Malhotra-Kumar, S.; Lammens, C.; Goossens, H.; Hryniewicz, W.; Brun-Buisson, C.; Carmeli, Y.; Gniadkowski, M.

In: Antimicrobial Agents and Chemotherapy, Vol. 57, No. 1, 01.2013, p. 309-316.

Research output: Contribution to journalArticle

Izdebski, R, Baraniak, A, Fiett, J, Adler, A, Kazma, M, Salomon, J, Lawrence, C, Rossini, A, Salvia, A, Vidal Samso, J, Fierro, J, Paul, M, Lerman, Y, Malhotra-Kumar, S, Lammens, C, Goossens, H, Hryniewicz, W, Brun-Buisson, C, Carmeli, Y & Gniadkowski, M 2013, 'Clonal structure, extended-spectrum β-lactamases,and acquired AmpC-type cephalosporinases of Escherichia coli populations colonizing patients in rehabilitation centers in four countries', Antimicrobial Agents and Chemotherapy, vol. 57, no. 1, pp. 309-316. https://doi.org/10.1128/AAC.01656-12
Izdebski, R. ; Baraniak, A. ; Fiett, J. ; Adler, A. ; Kazma, M. ; Salomon, J. ; Lawrence, C. ; Rossini, A. ; Salvia, A. ; Vidal Samso, J. ; Fierro, J. ; Paul, M. ; Lerman, Y. ; Malhotra-Kumar, S. ; Lammens, C. ; Goossens, H. ; Hryniewicz, W. ; Brun-Buisson, C. ; Carmeli, Y. ; Gniadkowski, M. / Clonal structure, extended-spectrum β-lactamases,and acquired AmpC-type cephalosporinases of Escherichia coli populations colonizing patients in rehabilitation centers in four countries. In: Antimicrobial Agents and Chemotherapy. 2013 ; Vol. 57, No. 1. pp. 309-316.
@article{3c434e408942473a875b8ace302dfbce,
title = "Clonal structure, extended-spectrum β-lactamases,and acquired AmpC-type cephalosporinases of Escherichia coli populations colonizing patients in rehabilitation centers in four countries",
abstract = "The prospective project MOSAR was conducted in five rehabilitation units: the Berck Maritime H{\^o}pital (Berck, France), Fondazione Santa Lucia (Rome, Italy), Guttmann Institute (GI; Barcelona, Spain), and Loewenstein Hospital and Tel-Aviv Souraski Medical Center (TA) (Tel-Aviv, Israel). Patients were screened for carriage of Enterobacteriaceae resistant to expanded-spectrum cephalosporins (ESCs) from admission until discharge. The aim of this study was to characterize the clonal structure, extended-spectrum β-lactamases (ESBLs), and acquired AmpC-like cephalosporinases in the Escherichia coli populations collected. A total of 376 isolates were randomly selected. The overall number of sequence types (STs) was 76, including 7 STs that grouped at least 10 isolates from at least three centers each, namely, STs 10, 38, 69, 131, 405, 410, and 648. These clones comprised 65.2{\%} of all isolates, and ST131 alone comprised 41.2{\%}. Of 54 STs observed only in one center, some STs played a locally significant role, like ST156 and ST393 in GI or ST372 and ST398 in TA. Among 16 new STs, five arose from evolution within the ST10 and ST131 clonal complexes. ESBLs and AmpCs accounted for 94.7{\%} and 5.6{\%} of the ESC-hydrolyzing β-lactamases, respectively, being dominated by the CTX-M-like enzymes (79.9{\%}), followed by the SHV (13.5{\%}) and CMY-2 (5.3{\%}) types. CTX-M-15 was the most prevalent β-lactamase overall (40.6{\%}); other ubiquitous enzymes were CTX-M-14 and CMY-2. Almost none of the common clones correlated strictly with one β-lactamase; although 58.7{\%} of ST131 isolates produced CTX-M-15, the clone also expressed nine other enzymes. A number of clone variants with specific pulsed-field gel electrophoresis and ESBL types were spread in some locales, potentially representing newly emerging E. coli epidemic strains.",
author = "R. Izdebski and A. Baraniak and J. Fiett and A. Adler and M. Kazma and J. Salomon and C. Lawrence and A. Rossini and A. Salvia and {Vidal Samso}, J. and J. Fierro and M. Paul and Y. Lerman and S. Malhotra-Kumar and C. Lammens and H. Goossens and W. Hryniewicz and C. Brun-Buisson and Y. Carmeli and M. Gniadkowski",
year = "2013",
month = "1",
doi = "10.1128/AAC.01656-12",
language = "English",
volume = "57",
pages = "309--316",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Clonal structure, extended-spectrum β-lactamases,and acquired AmpC-type cephalosporinases of Escherichia coli populations colonizing patients in rehabilitation centers in four countries

AU - Izdebski, R.

AU - Baraniak, A.

AU - Fiett, J.

AU - Adler, A.

AU - Kazma, M.

AU - Salomon, J.

AU - Lawrence, C.

AU - Rossini, A.

AU - Salvia, A.

AU - Vidal Samso, J.

AU - Fierro, J.

AU - Paul, M.

AU - Lerman, Y.

AU - Malhotra-Kumar, S.

AU - Lammens, C.

AU - Goossens, H.

AU - Hryniewicz, W.

AU - Brun-Buisson, C.

AU - Carmeli, Y.

AU - Gniadkowski, M.

PY - 2013/1

Y1 - 2013/1

N2 - The prospective project MOSAR was conducted in five rehabilitation units: the Berck Maritime Hôpital (Berck, France), Fondazione Santa Lucia (Rome, Italy), Guttmann Institute (GI; Barcelona, Spain), and Loewenstein Hospital and Tel-Aviv Souraski Medical Center (TA) (Tel-Aviv, Israel). Patients were screened for carriage of Enterobacteriaceae resistant to expanded-spectrum cephalosporins (ESCs) from admission until discharge. The aim of this study was to characterize the clonal structure, extended-spectrum β-lactamases (ESBLs), and acquired AmpC-like cephalosporinases in the Escherichia coli populations collected. A total of 376 isolates were randomly selected. The overall number of sequence types (STs) was 76, including 7 STs that grouped at least 10 isolates from at least three centers each, namely, STs 10, 38, 69, 131, 405, 410, and 648. These clones comprised 65.2% of all isolates, and ST131 alone comprised 41.2%. Of 54 STs observed only in one center, some STs played a locally significant role, like ST156 and ST393 in GI or ST372 and ST398 in TA. Among 16 new STs, five arose from evolution within the ST10 and ST131 clonal complexes. ESBLs and AmpCs accounted for 94.7% and 5.6% of the ESC-hydrolyzing β-lactamases, respectively, being dominated by the CTX-M-like enzymes (79.9%), followed by the SHV (13.5%) and CMY-2 (5.3%) types. CTX-M-15 was the most prevalent β-lactamase overall (40.6%); other ubiquitous enzymes were CTX-M-14 and CMY-2. Almost none of the common clones correlated strictly with one β-lactamase; although 58.7% of ST131 isolates produced CTX-M-15, the clone also expressed nine other enzymes. A number of clone variants with specific pulsed-field gel electrophoresis and ESBL types were spread in some locales, potentially representing newly emerging E. coli epidemic strains.

AB - The prospective project MOSAR was conducted in five rehabilitation units: the Berck Maritime Hôpital (Berck, France), Fondazione Santa Lucia (Rome, Italy), Guttmann Institute (GI; Barcelona, Spain), and Loewenstein Hospital and Tel-Aviv Souraski Medical Center (TA) (Tel-Aviv, Israel). Patients were screened for carriage of Enterobacteriaceae resistant to expanded-spectrum cephalosporins (ESCs) from admission until discharge. The aim of this study was to characterize the clonal structure, extended-spectrum β-lactamases (ESBLs), and acquired AmpC-like cephalosporinases in the Escherichia coli populations collected. A total of 376 isolates were randomly selected. The overall number of sequence types (STs) was 76, including 7 STs that grouped at least 10 isolates from at least three centers each, namely, STs 10, 38, 69, 131, 405, 410, and 648. These clones comprised 65.2% of all isolates, and ST131 alone comprised 41.2%. Of 54 STs observed only in one center, some STs played a locally significant role, like ST156 and ST393 in GI or ST372 and ST398 in TA. Among 16 new STs, five arose from evolution within the ST10 and ST131 clonal complexes. ESBLs and AmpCs accounted for 94.7% and 5.6% of the ESC-hydrolyzing β-lactamases, respectively, being dominated by the CTX-M-like enzymes (79.9%), followed by the SHV (13.5%) and CMY-2 (5.3%) types. CTX-M-15 was the most prevalent β-lactamase overall (40.6%); other ubiquitous enzymes were CTX-M-14 and CMY-2. Almost none of the common clones correlated strictly with one β-lactamase; although 58.7% of ST131 isolates produced CTX-M-15, the clone also expressed nine other enzymes. A number of clone variants with specific pulsed-field gel electrophoresis and ESBL types were spread in some locales, potentially representing newly emerging E. coli epidemic strains.

UR - http://www.scopus.com/inward/record.url?scp=84872023454&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872023454&partnerID=8YFLogxK

U2 - 10.1128/AAC.01656-12

DO - 10.1128/AAC.01656-12

M3 - Article

C2 - 23114774

AN - SCOPUS:84872023454

VL - 57

SP - 309

EP - 316

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 1

ER -